Brassinosteroids Positively Regulate Plant Immunity via BRI1-EMS-SUPPRESSOR 1-Mediated GLUCAN SYNTHASE-LIKE 8 Transcription
Plant hormone brassinosteroids (BRs) play key roles in plant adaptation to biotic stresses, including various pathogen infections. As a core factor in BR signaling, the transcription factor BRI1-EMS-SUPPRESSOR 1 (BES1) activates BR responses via regulating the expression of target genes. However, th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.854899/full |
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| author | Jiawei Xiong Xiaoping Wan Xiaoping Wan Xiaoping Wan Maolin Ran Maolin Ran Xiumei Xu Lezhang Chen Feng Yang Feng Yang Feng Yang |
| author_facet | Jiawei Xiong Xiaoping Wan Xiaoping Wan Xiaoping Wan Maolin Ran Maolin Ran Xiumei Xu Lezhang Chen Feng Yang Feng Yang Feng Yang |
| author_sort | Jiawei Xiong |
| collection | DOAJ |
| description | Plant hormone brassinosteroids (BRs) play key roles in plant adaptation to biotic stresses, including various pathogen infections. As a core factor in BR signaling, the transcription factor BRI1-EMS-SUPPRESSOR 1 (BES1) activates BR responses via regulating the expression of target genes. However, the molecular mechanism of BRs in regulating plant immunity is unclear, and the key components are not identified. In this study, we found that BR biosynthesis and signaling transduction are essential for plant resistance to pathogen infection, and BR biosynthesis or BR signaling-deficient mutants displayed susceptibility to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) infection [including more serious symptoms and more photosystem II (PSII) photochemistry damage]. We identified a callose synthase gene GLUCAN SYNTHASE-LIKE 8 (GSL8) as a direct target of BES1, and its expression was induced by BRs/BES1. Meanwhile, BRs induced callose accumulation after Pst DC3000 infection. Moreover, BES1 gain-of-function mutant bes1-D showed promoted Pst DC3000 resistance. GSL8 T-DNA insertion mutant gsl8-1 was susceptible to DC3000, while brassinolide (BL) treatment partially rescued gsl8-1 susceptible phenotypes. Our study suggests that BR-induced pathogen resistance partly depends on the BR-induced BES1-GSL8 cascade to mediate callose accumulation. |
| first_indexed | 2024-12-18T10:48:27Z |
| format | Article |
| id | doaj.art-3c93d6d52eda4fb7a97a9d11c812b631 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-18T10:48:27Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3c93d6d52eda4fb7a97a9d11c812b6312022-12-21T21:10:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-03-011310.3389/fpls.2022.854899854899Brassinosteroids Positively Regulate Plant Immunity via BRI1-EMS-SUPPRESSOR 1-Mediated GLUCAN SYNTHASE-LIKE 8 TranscriptionJiawei Xiong0Xiaoping Wan1Xiaoping Wan2Xiaoping Wan3Maolin Ran4Maolin Ran5Xiumei Xu6Lezhang Chen7Feng Yang8Feng Yang9Feng Yang10Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, ChinaKey Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, ChinaRice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang, ChinaInnovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaRice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang, ChinaVegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province, Chengdu, ChinaState Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, ChinaSichuan Huitai Agriculture Technology Co. Ltd., Chengdu, ChinaKey Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, ChinaRice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang, ChinaVegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province, Chengdu, ChinaPlant hormone brassinosteroids (BRs) play key roles in plant adaptation to biotic stresses, including various pathogen infections. As a core factor in BR signaling, the transcription factor BRI1-EMS-SUPPRESSOR 1 (BES1) activates BR responses via regulating the expression of target genes. However, the molecular mechanism of BRs in regulating plant immunity is unclear, and the key components are not identified. In this study, we found that BR biosynthesis and signaling transduction are essential for plant resistance to pathogen infection, and BR biosynthesis or BR signaling-deficient mutants displayed susceptibility to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) infection [including more serious symptoms and more photosystem II (PSII) photochemistry damage]. We identified a callose synthase gene GLUCAN SYNTHASE-LIKE 8 (GSL8) as a direct target of BES1, and its expression was induced by BRs/BES1. Meanwhile, BRs induced callose accumulation after Pst DC3000 infection. Moreover, BES1 gain-of-function mutant bes1-D showed promoted Pst DC3000 resistance. GSL8 T-DNA insertion mutant gsl8-1 was susceptible to DC3000, while brassinolide (BL) treatment partially rescued gsl8-1 susceptible phenotypes. Our study suggests that BR-induced pathogen resistance partly depends on the BR-induced BES1-GSL8 cascade to mediate callose accumulation.https://www.frontiersin.org/articles/10.3389/fpls.2022.854899/fullbrassinosteroidsPst DC3000Arabidopsis thalianaplant immunitycallose deposition |
| spellingShingle | Jiawei Xiong Xiaoping Wan Xiaoping Wan Xiaoping Wan Maolin Ran Maolin Ran Xiumei Xu Lezhang Chen Feng Yang Feng Yang Feng Yang Brassinosteroids Positively Regulate Plant Immunity via BRI1-EMS-SUPPRESSOR 1-Mediated GLUCAN SYNTHASE-LIKE 8 Transcription Frontiers in Plant Science brassinosteroids Pst DC3000 Arabidopsis thaliana plant immunity callose deposition |
| title | Brassinosteroids Positively Regulate Plant Immunity via BRI1-EMS-SUPPRESSOR 1-Mediated GLUCAN SYNTHASE-LIKE 8 Transcription |
| title_full | Brassinosteroids Positively Regulate Plant Immunity via BRI1-EMS-SUPPRESSOR 1-Mediated GLUCAN SYNTHASE-LIKE 8 Transcription |
| title_fullStr | Brassinosteroids Positively Regulate Plant Immunity via BRI1-EMS-SUPPRESSOR 1-Mediated GLUCAN SYNTHASE-LIKE 8 Transcription |
| title_full_unstemmed | Brassinosteroids Positively Regulate Plant Immunity via BRI1-EMS-SUPPRESSOR 1-Mediated GLUCAN SYNTHASE-LIKE 8 Transcription |
| title_short | Brassinosteroids Positively Regulate Plant Immunity via BRI1-EMS-SUPPRESSOR 1-Mediated GLUCAN SYNTHASE-LIKE 8 Transcription |
| title_sort | brassinosteroids positively regulate plant immunity via bri1 ems suppressor 1 mediated glucan synthase like 8 transcription |
| topic | brassinosteroids Pst DC3000 Arabidopsis thaliana plant immunity callose deposition |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.854899/full |
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