Laboratory indices of hospitalized sickle cell disease patients, prevalence and antimicrobial susceptibility of pathogenic bacterial isolates at MRCG ward in the Gambia
Abstract Background The aim of this study was to determine the prevalence of invasive bacterial infections and their antimicrobial resistance patterns in sickle cell disease (SCD) patients admitted at the Medical Research Council the Gambia (MRCG) Ward in the era of PCV and Hib vaccination in the Ga...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-023-08542-z |
| _version_ | 1797577872588668928 |
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| author | Mustapha Dibbasey Mamudou Dahaba Francess Sarfo Ida Jallow-Manneh Buntung Ceesay Solomon Umukoro Mouhamadou Fadel Diop Alfred Amambua-Ngwa |
| author_facet | Mustapha Dibbasey Mamudou Dahaba Francess Sarfo Ida Jallow-Manneh Buntung Ceesay Solomon Umukoro Mouhamadou Fadel Diop Alfred Amambua-Ngwa |
| author_sort | Mustapha Dibbasey |
| collection | DOAJ |
| description | Abstract Background The aim of this study was to determine the prevalence of invasive bacterial infections and their antimicrobial resistance patterns in sickle cell disease (SCD) patients admitted at the Medical Research Council the Gambia (MRCG) Ward in the era of PCV and Hib vaccination in the Gambia. Methods and Results This study was conducted in the clinical laboratory department of MRCG. We retrospectively generated haematological, and blood culture data from our electronic medical records from 2015 to 2022 of SCD patients admitted to MRCG Ward. Of 380 SCD patients, blood culture was requested only for 159. Of the 159 admitted SCD, 11 patients had qualified positive blood cultures. Five different types of bacterial pathogens were isolated from these positive blood cultures: 4 Staphylococcus aureus, 3 Streptococcus pneumoniae, 2 Salmonella species, 1 Enterococcus species, and 1 Shigella boydii. No episode of bacteremia caused by Haemophilus influenzae type b was identified. The molecular serotyping of the Streptococcus pneumoniae isolates revealed non-vaccine serotypes 10 A, 12 F and 12 F. Penicillin resistance was recorded in two of the three Streptococcus pneumoniae. The Staphylococcus aureus isolates were penicillin resistant but cefoxitin sensitive, hence no methicillin (oxacillin) resistant Staphylococcus aureus was reported. Generally, the isolated pathogens were all sensitive to chloramphenicol, and vancomycin. The haematological indices were not significantly varied between SCD patients with and without microbiologically confirmed bacterial infection. Conclusion Streptococcus pneumoniae and Staphylococcus aureus were the most common cause of bacteremia in these admitted SCD patients. The presence of non-typhoidal Salmonella and Shigella infection coupled with penicillin resistance should be considered during penicillin prophylaxis and empirical treatment regimens for SCD patients and future SCD management policies in the Gambia. The haematological parameters may not be reliable biomarkers in differentiating bacterial from non-bacterial infections in SCD patients. |
| first_indexed | 2024-03-10T22:14:07Z |
| format | Article |
| id | doaj.art-3c9a150f4e914824b3dfc72303a6df80 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-03-10T22:14:07Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-3c9a150f4e914824b3dfc72303a6df802023-11-19T12:29:26ZengBMCBMC Infectious Diseases1471-23342023-08-012311810.1186/s12879-023-08542-zLaboratory indices of hospitalized sickle cell disease patients, prevalence and antimicrobial susceptibility of pathogenic bacterial isolates at MRCG ward in the GambiaMustapha Dibbasey0Mamudou Dahaba1Francess Sarfo2Ida Jallow-Manneh3Buntung Ceesay4Solomon Umukoro5Mouhamadou Fadel Diop6Alfred Amambua-Ngwa7Department of Haematology laboratory, Clinical Laboratory, Medical Research Council the Gambia at London School of Hygiene and Tropical MedicineDepartment of Haematology laboratory, Clinical Laboratory, Medical Research Council the Gambia at London School of Hygiene and Tropical MedicineDepartment of Haematology laboratory, Clinical Laboratory, Medical Research Council the Gambia at London School of Hygiene and Tropical MedicineDepartment of Haematology laboratory, Clinical Laboratory, Medical Research Council the Gambia at London School of Hygiene and Tropical MedicineDepartment of Clinical Microbiology laboratory, Clinical Laboratory, Medical Research Council the Gambia at London School of Hygiene and Tropical MedicineClinical Laboratory Department, Medical Research Council the Gambia at London School of Hygiene and Tropical MedicineDepartment of Data Science, Medical Research Council the Gambia at London School of Hygiene and Tropical MedicineMalaria Biology Group Department, Medical Research Council the Gambia at London School of Hygiene and Tropical MedicineAbstract Background The aim of this study was to determine the prevalence of invasive bacterial infections and their antimicrobial resistance patterns in sickle cell disease (SCD) patients admitted at the Medical Research Council the Gambia (MRCG) Ward in the era of PCV and Hib vaccination in the Gambia. Methods and Results This study was conducted in the clinical laboratory department of MRCG. We retrospectively generated haematological, and blood culture data from our electronic medical records from 2015 to 2022 of SCD patients admitted to MRCG Ward. Of 380 SCD patients, blood culture was requested only for 159. Of the 159 admitted SCD, 11 patients had qualified positive blood cultures. Five different types of bacterial pathogens were isolated from these positive blood cultures: 4 Staphylococcus aureus, 3 Streptococcus pneumoniae, 2 Salmonella species, 1 Enterococcus species, and 1 Shigella boydii. No episode of bacteremia caused by Haemophilus influenzae type b was identified. The molecular serotyping of the Streptococcus pneumoniae isolates revealed non-vaccine serotypes 10 A, 12 F and 12 F. Penicillin resistance was recorded in two of the three Streptococcus pneumoniae. The Staphylococcus aureus isolates were penicillin resistant but cefoxitin sensitive, hence no methicillin (oxacillin) resistant Staphylococcus aureus was reported. Generally, the isolated pathogens were all sensitive to chloramphenicol, and vancomycin. The haematological indices were not significantly varied between SCD patients with and without microbiologically confirmed bacterial infection. Conclusion Streptococcus pneumoniae and Staphylococcus aureus were the most common cause of bacteremia in these admitted SCD patients. The presence of non-typhoidal Salmonella and Shigella infection coupled with penicillin resistance should be considered during penicillin prophylaxis and empirical treatment regimens for SCD patients and future SCD management policies in the Gambia. The haematological parameters may not be reliable biomarkers in differentiating bacterial from non-bacterial infections in SCD patients.https://doi.org/10.1186/s12879-023-08542-zSickle cell diseaseBacteraemiaBacterial infectionsAntimicrobial resistance patternsHaematological parameters |
| spellingShingle | Mustapha Dibbasey Mamudou Dahaba Francess Sarfo Ida Jallow-Manneh Buntung Ceesay Solomon Umukoro Mouhamadou Fadel Diop Alfred Amambua-Ngwa Laboratory indices of hospitalized sickle cell disease patients, prevalence and antimicrobial susceptibility of pathogenic bacterial isolates at MRCG ward in the Gambia BMC Infectious Diseases Sickle cell disease Bacteraemia Bacterial infections Antimicrobial resistance patterns Haematological parameters |
| title | Laboratory indices of hospitalized sickle cell disease patients, prevalence and antimicrobial susceptibility of pathogenic bacterial isolates at MRCG ward in the Gambia |
| title_full | Laboratory indices of hospitalized sickle cell disease patients, prevalence and antimicrobial susceptibility of pathogenic bacterial isolates at MRCG ward in the Gambia |
| title_fullStr | Laboratory indices of hospitalized sickle cell disease patients, prevalence and antimicrobial susceptibility of pathogenic bacterial isolates at MRCG ward in the Gambia |
| title_full_unstemmed | Laboratory indices of hospitalized sickle cell disease patients, prevalence and antimicrobial susceptibility of pathogenic bacterial isolates at MRCG ward in the Gambia |
| title_short | Laboratory indices of hospitalized sickle cell disease patients, prevalence and antimicrobial susceptibility of pathogenic bacterial isolates at MRCG ward in the Gambia |
| title_sort | laboratory indices of hospitalized sickle cell disease patients prevalence and antimicrobial susceptibility of pathogenic bacterial isolates at mrcg ward in the gambia |
| topic | Sickle cell disease Bacteraemia Bacterial infections Antimicrobial resistance patterns Haematological parameters |
| url | https://doi.org/10.1186/s12879-023-08542-z |
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