Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctions
Synaptic vesicles can be released at extremely high rates, which places an extraordinary demand on the recycling machinery. Previous ultrastructural studies of vesicle recycling were conducted in dissected preparations using an intense stimulation to maximize the probability of release. Here, a sing...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2013-09-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/00723 |
| _version_ | 1811236868011851776 |
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| author | Shigeki Watanabe Qiang Liu M Wayne Davis Gunther Hollopeter Nikita Thomas Nels B Jorgensen Erik M Jorgensen |
| author_facet | Shigeki Watanabe Qiang Liu M Wayne Davis Gunther Hollopeter Nikita Thomas Nels B Jorgensen Erik M Jorgensen |
| author_sort | Shigeki Watanabe |
| collection | DOAJ |
| description | Synaptic vesicles can be released at extremely high rates, which places an extraordinary demand on the recycling machinery. Previous ultrastructural studies of vesicle recycling were conducted in dissected preparations using an intense stimulation to maximize the probability of release. Here, a single light stimulus was applied to motor neurons in intact Caenorhabditis elegans nematodes expressing channelrhodopsin, and the animals rapidly frozen. We found that docked vesicles fuse along a broad active zone in response to a single stimulus, and are replenished with a time constant of about 2 s. Endocytosis occurs within 50 ms adjacent to the dense projection and after 1 s adjacent to adherens junctions. These studies suggest that synaptic vesicle endocytosis may occur on a millisecond time scale following a single physiological stimulus in the intact nervous system and is unlikely to conform to current models of endocytosis. |
| first_indexed | 2024-04-12T12:15:29Z |
| format | Article |
| id | doaj.art-3c9b8f7c312a4155964027368d6deb0d |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:15:29Z |
| publishDate | 2013-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-3c9b8f7c312a4155964027368d6deb0d2022-12-22T03:33:26ZengeLife Sciences Publications LtdeLife2050-084X2013-09-01210.7554/eLife.00723Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctionsShigeki Watanabe0Qiang Liu1M Wayne Davis2Gunther Hollopeter3Nikita Thomas4Nels B Jorgensen5Erik M Jorgensen6Department of Biology, Howard Hughes Medical Institute, University of Utah, Salt Lake City, United StatesDepartment of Biology, Howard Hughes Medical Institute, University of Utah, Salt Lake City, United StatesDepartment of Biology, Howard Hughes Medical Institute, University of Utah, Salt Lake City, United StatesDepartment of Biology, Howard Hughes Medical Institute, University of Utah, Salt Lake City, United StatesDepartment of Biology, Howard Hughes Medical Institute, University of Utah, Salt Lake City, United StatesDepartment of Biology, Howard Hughes Medical Institute, University of Utah, Salt Lake City, United StatesDepartment of Biology, Howard Hughes Medical Institute, University of Utah, Salt Lake City, United StatesSynaptic vesicles can be released at extremely high rates, which places an extraordinary demand on the recycling machinery. Previous ultrastructural studies of vesicle recycling were conducted in dissected preparations using an intense stimulation to maximize the probability of release. Here, a single light stimulus was applied to motor neurons in intact Caenorhabditis elegans nematodes expressing channelrhodopsin, and the animals rapidly frozen. We found that docked vesicles fuse along a broad active zone in response to a single stimulus, and are replenished with a time constant of about 2 s. Endocytosis occurs within 50 ms adjacent to the dense projection and after 1 s adjacent to adherens junctions. These studies suggest that synaptic vesicle endocytosis may occur on a millisecond time scale following a single physiological stimulus in the intact nervous system and is unlikely to conform to current models of endocytosis.https://elifesciences.org/articles/00723synaptic vesicle endocytosisoptogeneticstime-resolved electron microscopyhigh-pressure freezingsynaptic vesicle exocytosisactive zone |
| spellingShingle | Shigeki Watanabe Qiang Liu M Wayne Davis Gunther Hollopeter Nikita Thomas Nels B Jorgensen Erik M Jorgensen Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctions eLife synaptic vesicle endocytosis optogenetics time-resolved electron microscopy high-pressure freezing synaptic vesicle exocytosis active zone |
| title | Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctions |
| title_full | Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctions |
| title_fullStr | Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctions |
| title_full_unstemmed | Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctions |
| title_short | Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctions |
| title_sort | ultrafast endocytosis at caenorhabditis elegans neuromuscular junctions |
| topic | synaptic vesicle endocytosis optogenetics time-resolved electron microscopy high-pressure freezing synaptic vesicle exocytosis active zone |
| url | https://elifesciences.org/articles/00723 |
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