In Vitro Interactions of TiO<sub>2</sub> Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment
Titanium dioxide nanoparticles (TiO<sub>2</sub> NPs) are manufactured worldwide. Once they arrive in the soil environment, they can endanger living organisms. Hence, monitoring and assessing the effects of these nanoparticles is required. We focus on the <i>Eisenia andrei</i>...
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MDPI AG
2021-01-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/1/250 |
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| author | Natividad Isabel Navarro Pacheco Radka Roubalova Jaroslav Semerad Alena Grasserova Oldrich Benada Olga Kofronova Tomas Cajthaml Jiri Dvorak Martin Bilej Petra Prochazkova |
| author_facet | Natividad Isabel Navarro Pacheco Radka Roubalova Jaroslav Semerad Alena Grasserova Oldrich Benada Olga Kofronova Tomas Cajthaml Jiri Dvorak Martin Bilej Petra Prochazkova |
| author_sort | Natividad Isabel Navarro Pacheco |
| collection | DOAJ |
| description | Titanium dioxide nanoparticles (TiO<sub>2</sub> NPs) are manufactured worldwide. Once they arrive in the soil environment, they can endanger living organisms. Hence, monitoring and assessing the effects of these nanoparticles is required. We focus on the <i>Eisenia andrei</i> earthworm immune cells exposed to sublethal concentrations of TiO<sub>2</sub> NPs (1, 10, and 100 µg/mL) for 2, 6, and 24 h. TiO<sub>2</sub> NPs at all concentrations did not affect cell viability. Further, TiO<sub>2</sub> NPs did not cause changes in reactive oxygen species (ROS) production, malondialdehyde (MDA) production, and phagocytic activity. Similarly, they did not elicit DNA damage. Overall, we did not detect any toxic effects of TiO<sub>2</sub> NPs at the cellular level. At the gene expression level, slight changes were detected. Metallothionein, fetidin/lysenin, lumbricin and MEK kinase I were upregulated in coelomocytes after exposure to 10 µg/mL TiO<sub>2</sub> NPs for 6 h. Antioxidant enzyme expression was similar in exposed and control cells. TiO<sub>2</sub> NPs were detected on coelomocyte membranes. However, our results do not show any strong effects of these nanoparticles on coelomocytes at both the cellular and molecular levels. |
| first_indexed | 2024-03-09T04:21:45Z |
| format | Article |
| id | doaj.art-3c9f4ebef6fd410b98dd4607f0636a84 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T04:21:45Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-3c9f4ebef6fd410b98dd4607f0636a842023-12-03T13:45:27ZengMDPI AGNanomaterials2079-49912021-01-0111125010.3390/nano11010250In Vitro Interactions of TiO<sub>2</sub> Nanoparticles with Earthworm Coelomocytes: Immunotoxicity AssessmentNatividad Isabel Navarro Pacheco0Radka Roubalova1Jaroslav Semerad2Alena Grasserova3Oldrich Benada4Olga Kofronova5Tomas Cajthaml6Jiri Dvorak7Martin Bilej8Petra Prochazkova9Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech RepublicInstitute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech RepublicTitanium dioxide nanoparticles (TiO<sub>2</sub> NPs) are manufactured worldwide. Once they arrive in the soil environment, they can endanger living organisms. Hence, monitoring and assessing the effects of these nanoparticles is required. We focus on the <i>Eisenia andrei</i> earthworm immune cells exposed to sublethal concentrations of TiO<sub>2</sub> NPs (1, 10, and 100 µg/mL) for 2, 6, and 24 h. TiO<sub>2</sub> NPs at all concentrations did not affect cell viability. Further, TiO<sub>2</sub> NPs did not cause changes in reactive oxygen species (ROS) production, malondialdehyde (MDA) production, and phagocytic activity. Similarly, they did not elicit DNA damage. Overall, we did not detect any toxic effects of TiO<sub>2</sub> NPs at the cellular level. At the gene expression level, slight changes were detected. Metallothionein, fetidin/lysenin, lumbricin and MEK kinase I were upregulated in coelomocytes after exposure to 10 µg/mL TiO<sub>2</sub> NPs for 6 h. Antioxidant enzyme expression was similar in exposed and control cells. TiO<sub>2</sub> NPs were detected on coelomocyte membranes. However, our results do not show any strong effects of these nanoparticles on coelomocytes at both the cellular and molecular levels.https://www.mdpi.com/2079-4991/11/1/250earthwormcoelomocyteTiO<sub>2</sub> nanoparticlesreactive oxygen speciesinnate immunitylipid peroxidation |
| spellingShingle | Natividad Isabel Navarro Pacheco Radka Roubalova Jaroslav Semerad Alena Grasserova Oldrich Benada Olga Kofronova Tomas Cajthaml Jiri Dvorak Martin Bilej Petra Prochazkova In Vitro Interactions of TiO<sub>2</sub> Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment Nanomaterials earthworm coelomocyte TiO<sub>2</sub> nanoparticles reactive oxygen species innate immunity lipid peroxidation |
| title | In Vitro Interactions of TiO<sub>2</sub> Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment |
| title_full | In Vitro Interactions of TiO<sub>2</sub> Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment |
| title_fullStr | In Vitro Interactions of TiO<sub>2</sub> Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment |
| title_full_unstemmed | In Vitro Interactions of TiO<sub>2</sub> Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment |
| title_short | In Vitro Interactions of TiO<sub>2</sub> Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment |
| title_sort | in vitro interactions of tio sub 2 sub nanoparticles with earthworm coelomocytes immunotoxicity assessment |
| topic | earthworm coelomocyte TiO<sub>2</sub> nanoparticles reactive oxygen species innate immunity lipid peroxidation |
| url | https://www.mdpi.com/2079-4991/11/1/250 |
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