Metabolomics Reveals the Alteration of Metabolic Pathway by Alpha-Melanocyte-Stimulating Hormone in B16F10 Melanoma Cells
The purpose of this study was to understand the changes of metabolic pathway induced by alpha-melanocyte-stimulating hormone (α-MSH) in B16F10 melanoma cells in an untargeted metabolomics approach. Cells were treated with 100 nM of α-MSH and then incubated for 48 h. α-MSH increased tyrosinase activi...
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MDPI AG
2020-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/15/3384 |
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| author | Seung-Ho Seo Jae Kwon Jo Eun-Ju Kim Seong-Eun Park Seo Yeon Shin Kyung Mok Park Hong-Seok Son |
| author_facet | Seung-Ho Seo Jae Kwon Jo Eun-Ju Kim Seong-Eun Park Seo Yeon Shin Kyung Mok Park Hong-Seok Son |
| author_sort | Seung-Ho Seo |
| collection | DOAJ |
| description | The purpose of this study was to understand the changes of metabolic pathway induced by alpha-melanocyte-stimulating hormone (α-MSH) in B16F10 melanoma cells in an untargeted metabolomics approach. Cells were treated with 100 nM of α-MSH and then incubated for 48 h. α-MSH increased tyrosinase activity and melanin content by 56.5 and 61.7%, respectively, compared to untreated cells after 48 h of cultivation. The clear separation between groups was observed in the principal component analysis score plot, indicating that the levels of metabolites of melanoma cells were altered by treatment with α-MSH. Metabolic pathways affected by α-MSH were involved in some amino acid metabolisms. The increased levels of fumaric acid, malic acid, oxaloacetic acid and citric acid related to the citric acid cycle pathway after α-MSH treatment suggested enhanced energy metabolism. Metabolic pathways altered by α-MSH treatment can provide useful information to develop new skin pigmentation inhibitors or anti-obesity drugs. |
| first_indexed | 2024-03-10T18:11:52Z |
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| id | doaj.art-3ca0047d1f24445a9c2d33e524d489c6 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T18:11:52Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3ca0047d1f24445a9c2d33e524d489c62023-11-20T08:01:38ZengMDPI AGMolecules1420-30492020-07-012515338410.3390/molecules25153384Metabolomics Reveals the Alteration of Metabolic Pathway by Alpha-Melanocyte-Stimulating Hormone in B16F10 Melanoma CellsSeung-Ho Seo0Jae Kwon Jo1Eun-Ju Kim2Seong-Eun Park3Seo Yeon Shin4Kyung Mok Park5Hong-Seok Son6School of Korean Medicine, Dongshin University, Naju, Jeonnam 58245, KoreaSchool of Korean Medicine, Dongshin University, Naju, Jeonnam 58245, KoreaSchool of Korean Medicine, Dongshin University, Naju, Jeonnam 58245, KoreaSchool of Korean Medicine, Dongshin University, Naju, Jeonnam 58245, KoreaDepartment of Pharmaceutical Engineering, Dongshin University, Naju, Jeonnam 58245, KoreaDepartment of Pharmaceutical Engineering, Dongshin University, Naju, Jeonnam 58245, KoreaSchool of Korean Medicine, Dongshin University, Naju, Jeonnam 58245, KoreaThe purpose of this study was to understand the changes of metabolic pathway induced by alpha-melanocyte-stimulating hormone (α-MSH) in B16F10 melanoma cells in an untargeted metabolomics approach. Cells were treated with 100 nM of α-MSH and then incubated for 48 h. α-MSH increased tyrosinase activity and melanin content by 56.5 and 61.7%, respectively, compared to untreated cells after 48 h of cultivation. The clear separation between groups was observed in the principal component analysis score plot, indicating that the levels of metabolites of melanoma cells were altered by treatment with α-MSH. Metabolic pathways affected by α-MSH were involved in some amino acid metabolisms. The increased levels of fumaric acid, malic acid, oxaloacetic acid and citric acid related to the citric acid cycle pathway after α-MSH treatment suggested enhanced energy metabolism. Metabolic pathways altered by α-MSH treatment can provide useful information to develop new skin pigmentation inhibitors or anti-obesity drugs.https://www.mdpi.com/1420-3049/25/15/3384α-melanocyte-stimulating hormonemelanoma cellmetabolomicsenergy metabolismamino acid metabolism |
| spellingShingle | Seung-Ho Seo Jae Kwon Jo Eun-Ju Kim Seong-Eun Park Seo Yeon Shin Kyung Mok Park Hong-Seok Son Metabolomics Reveals the Alteration of Metabolic Pathway by Alpha-Melanocyte-Stimulating Hormone in B16F10 Melanoma Cells Molecules α-melanocyte-stimulating hormone melanoma cell metabolomics energy metabolism amino acid metabolism |
| title | Metabolomics Reveals the Alteration of Metabolic Pathway by Alpha-Melanocyte-Stimulating Hormone in B16F10 Melanoma Cells |
| title_full | Metabolomics Reveals the Alteration of Metabolic Pathway by Alpha-Melanocyte-Stimulating Hormone in B16F10 Melanoma Cells |
| title_fullStr | Metabolomics Reveals the Alteration of Metabolic Pathway by Alpha-Melanocyte-Stimulating Hormone in B16F10 Melanoma Cells |
| title_full_unstemmed | Metabolomics Reveals the Alteration of Metabolic Pathway by Alpha-Melanocyte-Stimulating Hormone in B16F10 Melanoma Cells |
| title_short | Metabolomics Reveals the Alteration of Metabolic Pathway by Alpha-Melanocyte-Stimulating Hormone in B16F10 Melanoma Cells |
| title_sort | metabolomics reveals the alteration of metabolic pathway by alpha melanocyte stimulating hormone in b16f10 melanoma cells |
| topic | α-melanocyte-stimulating hormone melanoma cell metabolomics energy metabolism amino acid metabolism |
| url | https://www.mdpi.com/1420-3049/25/15/3384 |
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