Next-Generation Sequencing and Influenza Virus: A Short Review of the Published Implementation Attempts
Influenza virus represents a major public health concern worldwide after recent pandemics. To aid the understanding and characterization of the virus in ever-increasing sample numbers, new research techniques have been used, such as next-generation sequencing (NGS). The current article review used O...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bogor Agricultural University
2016-10-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1978301916303060 |
| _version_ | 1831750774266265600 |
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| author | Rasha Ali Ruth Marion Blackburn Zisis Kozlakidis |
| author_facet | Rasha Ali Ruth Marion Blackburn Zisis Kozlakidis |
| author_sort | Rasha Ali |
| collection | DOAJ |
| description | Influenza virus represents a major public health concern worldwide after recent pandemics. To aid the understanding and characterization of the virus in ever-increasing sample numbers, new research techniques have been used, such as next-generation sequencing (NGS). The current article review used Ovid MEDLINE and PubMed databases to conduct keyword searches and investigate the extent to which published NGS high-throughput approaches have been implemented to influenza virus research in the last 5 years, during which the increase in research funding for influenza studies has been coincidental with a significant per-base cost reduction of sequencing. Through the current literature review, it is evident that over the last 5 years, NGS techniques have been indeed applied to biological and clinical samples at increasing rates following a wide variety of approaches. The rate of adoption is slower than anticipated by most published studies, with three obstacles identified consistently by authors. These are the lack of suitable downstream analytical capacity, the absence of established quality control comparators, and the higher cost to comparable existing techniques. |
| first_indexed | 2024-12-21T22:33:32Z |
| format | Article |
| id | doaj.art-3ca1b2c3b17842a4961cbaa20c5e1993 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 |
| language | English |
| last_indexed | 2024-12-21T22:33:32Z |
| publishDate | 2016-10-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-3ca1b2c3b17842a4961cbaa20c5e19932022-12-21T18:48:01ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192016-10-0123415515910.1016/j.hjb.2016.12.007Next-Generation Sequencing and Influenza Virus: A Short Review of the Published Implementation AttemptsRasha Ali0Ruth Marion Blackburn1Zisis Kozlakidis2Division of Infection and Immunity, University College London, London, United KingdomFarr Institute of Health Informatics Research, University College London, London, United KingdomDivision of Infection and Immunity, University College London, London, United KingdomInfluenza virus represents a major public health concern worldwide after recent pandemics. To aid the understanding and characterization of the virus in ever-increasing sample numbers, new research techniques have been used, such as next-generation sequencing (NGS). The current article review used Ovid MEDLINE and PubMed databases to conduct keyword searches and investigate the extent to which published NGS high-throughput approaches have been implemented to influenza virus research in the last 5 years, during which the increase in research funding for influenza studies has been coincidental with a significant per-base cost reduction of sequencing. Through the current literature review, it is evident that over the last 5 years, NGS techniques have been indeed applied to biological and clinical samples at increasing rates following a wide variety of approaches. The rate of adoption is slower than anticipated by most published studies, with three obstacles identified consistently by authors. These are the lack of suitable downstream analytical capacity, the absence of established quality control comparators, and the higher cost to comparable existing techniques.http://www.sciencedirect.com/science/article/pii/S1978301916303060implementationinfluenzanext generation sequencing |
| spellingShingle | Rasha Ali Ruth Marion Blackburn Zisis Kozlakidis Next-Generation Sequencing and Influenza Virus: A Short Review of the Published Implementation Attempts Hayati Journal of Biosciences implementation influenza next generation sequencing |
| title | Next-Generation Sequencing and Influenza Virus: A Short Review of the Published Implementation Attempts |
| title_full | Next-Generation Sequencing and Influenza Virus: A Short Review of the Published Implementation Attempts |
| title_fullStr | Next-Generation Sequencing and Influenza Virus: A Short Review of the Published Implementation Attempts |
| title_full_unstemmed | Next-Generation Sequencing and Influenza Virus: A Short Review of the Published Implementation Attempts |
| title_short | Next-Generation Sequencing and Influenza Virus: A Short Review of the Published Implementation Attempts |
| title_sort | next generation sequencing and influenza virus a short review of the published implementation attempts |
| topic | implementation influenza next generation sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S1978301916303060 |
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