Identification of N6-Methyladenosine-Related lncRNAs as a Prognostic Signature in Glioma
N6-methyladenosine (m6A) modification is the most abundant modification in long noncoding RNAs (lncRNAs). Current studies have shown that the abnormal expression of m6A-related genes is closely associated with the tumorigenesis and progression of glioma. However, the role of m6A-related lncRNAs in g...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.789283/full |
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| author | Yujia Chen Yuduo Guo Shenglun Li Jiacheng Xu Xiang Wang Weihai Ning Lixin Ma Yanming Qu Mingshan Zhang Hongwei Zhang |
| author_facet | Yujia Chen Yuduo Guo Shenglun Li Jiacheng Xu Xiang Wang Weihai Ning Lixin Ma Yanming Qu Mingshan Zhang Hongwei Zhang |
| author_sort | Yujia Chen |
| collection | DOAJ |
| description | N6-methyladenosine (m6A) modification is the most abundant modification in long noncoding RNAs (lncRNAs). Current studies have shown that the abnormal expression of m6A-related genes is closely associated with the tumorigenesis and progression of glioma. However, the role of m6A-related lncRNAs in glioma development is still unclear. Herein, we screened 566 m6A-related lncRNAs in glioma from The Cancer Genome Atlas (TCGA) database. The expression pattern of these lncRNAs could cluster samples into two groups, in which various classical tumor-related functions and the tumor immune microenvironment were significantly different. Subsequently, a nine-factor m6A-related lncRNA prognostic signature (MLPS) was constructed by using a LASSO regression analysis in the training set and was validated in the test set and independent datasets. The AUC values of the MLPS were 0.881, 0.918 and 0.887 for 1-, 3- and 5-year survival in the training set, respectively, and 0.856, 0.916 and 0.909 for 1-, 3-, and 5-year survival in the test set, respectively. Stratification analyses of the MLPS illustrated its prognostic performance in gliomas with different characteristics. Correlation analyses showed that the infiltrations of monocytes and tumor-associated macrophages (TAMs) were significantly relevant to the risk score in the MLPS. Moreover, we detected the expression of four MLPS factors with defined sequences in glioma and normal cells by using RT–PCR. Afterwards, we investigated the functions of LNCTAM34A (one of the MLPS factors) in glioma cells, which have rarely been reported. Via in vitro experiments, LNCTAM34A was demonstrated to promote the proliferation, migration and epithelial-mesenchymal transition (EMT) of glioma cells. Overall, our study revealed the critical role of m6A-related lncRNAs in glioma and elucidated that LNCTAM34A could promote glioma proliferation, migration and EMT. |
| first_indexed | 2024-12-12T02:22:21Z |
| format | Article |
| id | doaj.art-3ca3f4fd8592443189a74b2603e84d77 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-12T02:22:21Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-3ca3f4fd8592443189a74b2603e84d772022-12-22T00:41:38ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-03-011210.3389/fonc.2022.789283789283Identification of N6-Methyladenosine-Related lncRNAs as a Prognostic Signature in GliomaYujia Chen0Yuduo Guo1Shenglun Li2Jiacheng Xu3Xiang Wang4Weihai Ning5Lixin Ma6Yanming Qu7Mingshan Zhang8Hongwei Zhang9Department of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, ChinaCAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, ChinaDepartment of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, ChinaDepartment of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, ChinaDepartment of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, ChinaDepartment of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, ChinaDepartment of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, ChinaDepartment of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, ChinaDepartment of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, ChinaDepartment of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, ChinaN6-methyladenosine (m6A) modification is the most abundant modification in long noncoding RNAs (lncRNAs). Current studies have shown that the abnormal expression of m6A-related genes is closely associated with the tumorigenesis and progression of glioma. However, the role of m6A-related lncRNAs in glioma development is still unclear. Herein, we screened 566 m6A-related lncRNAs in glioma from The Cancer Genome Atlas (TCGA) database. The expression pattern of these lncRNAs could cluster samples into two groups, in which various classical tumor-related functions and the tumor immune microenvironment were significantly different. Subsequently, a nine-factor m6A-related lncRNA prognostic signature (MLPS) was constructed by using a LASSO regression analysis in the training set and was validated in the test set and independent datasets. The AUC values of the MLPS were 0.881, 0.918 and 0.887 for 1-, 3- and 5-year survival in the training set, respectively, and 0.856, 0.916 and 0.909 for 1-, 3-, and 5-year survival in the test set, respectively. Stratification analyses of the MLPS illustrated its prognostic performance in gliomas with different characteristics. Correlation analyses showed that the infiltrations of monocytes and tumor-associated macrophages (TAMs) were significantly relevant to the risk score in the MLPS. Moreover, we detected the expression of four MLPS factors with defined sequences in glioma and normal cells by using RT–PCR. Afterwards, we investigated the functions of LNCTAM34A (one of the MLPS factors) in glioma cells, which have rarely been reported. Via in vitro experiments, LNCTAM34A was demonstrated to promote the proliferation, migration and epithelial-mesenchymal transition (EMT) of glioma cells. Overall, our study revealed the critical role of m6A-related lncRNAs in glioma and elucidated that LNCTAM34A could promote glioma proliferation, migration and EMT.https://www.frontiersin.org/articles/10.3389/fonc.2022.789283/fullThe Cancer Genome AtlasN6-methyladenosinelong non-coding RNAprognostic signatureglioma |
| spellingShingle | Yujia Chen Yuduo Guo Shenglun Li Jiacheng Xu Xiang Wang Weihai Ning Lixin Ma Yanming Qu Mingshan Zhang Hongwei Zhang Identification of N6-Methyladenosine-Related lncRNAs as a Prognostic Signature in Glioma Frontiers in Oncology The Cancer Genome Atlas N6-methyladenosine long non-coding RNA prognostic signature glioma |
| title | Identification of N6-Methyladenosine-Related lncRNAs as a Prognostic Signature in Glioma |
| title_full | Identification of N6-Methyladenosine-Related lncRNAs as a Prognostic Signature in Glioma |
| title_fullStr | Identification of N6-Methyladenosine-Related lncRNAs as a Prognostic Signature in Glioma |
| title_full_unstemmed | Identification of N6-Methyladenosine-Related lncRNAs as a Prognostic Signature in Glioma |
| title_short | Identification of N6-Methyladenosine-Related lncRNAs as a Prognostic Signature in Glioma |
| title_sort | identification of n6 methyladenosine related lncrnas as a prognostic signature in glioma |
| topic | The Cancer Genome Atlas N6-methyladenosine long non-coding RNA prognostic signature glioma |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.789283/full |
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