Over-Expression of a Wheat Late Maturity Alpha-Amylase Type 1 Impact on Starch Properties During Grain Development and Germination
The hydrolysis of starch is a complex process that requires synergistic action of multiple hydrolytic enzymes, including α-amylases. Wheat over-expression of TaAmy1, driven by seed specific promoter, resulted in a 20- to 230-fold total α-amylase activity in mature grains. Ectopic expression of TaAmy...
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| Format: | Article |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.811728/full |
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| author | Qin Zhang Qin Zhang Jenifer Pritchard Jos Mieog Jos Mieog Keren Byrne Keren Byrne Michelle L. Colgrave Michelle L. Colgrave Ji-Rui Wang Jean-Philippe F. Ral |
| author_facet | Qin Zhang Qin Zhang Jenifer Pritchard Jos Mieog Jos Mieog Keren Byrne Keren Byrne Michelle L. Colgrave Michelle L. Colgrave Ji-Rui Wang Jean-Philippe F. Ral |
| author_sort | Qin Zhang |
| collection | DOAJ |
| description | The hydrolysis of starch is a complex process that requires synergistic action of multiple hydrolytic enzymes, including α-amylases. Wheat over-expression of TaAmy1, driven by seed specific promoter, resulted in a 20- to 230-fold total α-amylase activity in mature grains. Ectopic expression of TaAmy1 showed a significant elevated α-amylase activity in stem and leaf without consequences on transitory starch. In mature grain, overexpressed TaAMY1 was mainly located in the endosperm with high expression of TaAmy1. This is due to early developing grains having effect on starch granules from 18 days post-anthesis (DPA) and on soluble sugar accumulation from 30 DPA. While accumulation of TaAMY1 led to a high degree of damaged starch in grain, the dramatic alterations of starch visco-properties caused by the elevated levels of α-amylase essentially occurred during processing, thus suggesting a very small impact of related starch damage on grain properties. Abnormal accumulation of soluble sugar (α-gluco-oligosaccharide and sucrose) by TaAMY1 over-expression reduced the grain dormancy and enhanced abscisic acid (ABA) resistance. Germination study in the presence of α-amylase inhibitor suggested a very limited role of TaAMY1 in the early germination process and starch conversion into soluble sugars. |
| first_indexed | 2024-12-18T05:40:58Z |
| format | Article |
| id | doaj.art-3ca3fd3d95024ccb97bcc4fc2dc89a98 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-18T05:40:58Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3ca3fd3d95024ccb97bcc4fc2dc89a982022-12-21T21:19:11ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-03-011310.3389/fpls.2022.811728811728Over-Expression of a Wheat Late Maturity Alpha-Amylase Type 1 Impact on Starch Properties During Grain Development and GerminationQin Zhang0Qin Zhang1Jenifer Pritchard2Jos Mieog3Jos Mieog4Keren Byrne5Keren Byrne6Michelle L. Colgrave7Michelle L. Colgrave8Ji-Rui Wang9Jean-Philippe F. Ral10Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Canberra, ACT, AustraliaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaAgriculture and Food, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Canberra, ACT, AustraliaAgriculture and Food, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Canberra, ACT, AustraliaSouthern Cross Plant Science, Southern Cross University, Lismore, NSW, AustraliaAgriculture and Food, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Canberra, ACT, AustraliaAgriculture and Food, Commonwealth Scientific and Industrial Research Organisation (CSIRO), St Lucia, QLD, AustraliaAgriculture and Food, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Canberra, ACT, AustraliaAgriculture and Food, Commonwealth Scientific and Industrial Research Organisation (CSIRO), St Lucia, QLD, AustraliaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaAgriculture and Food, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Canberra, ACT, AustraliaThe hydrolysis of starch is a complex process that requires synergistic action of multiple hydrolytic enzymes, including α-amylases. Wheat over-expression of TaAmy1, driven by seed specific promoter, resulted in a 20- to 230-fold total α-amylase activity in mature grains. Ectopic expression of TaAmy1 showed a significant elevated α-amylase activity in stem and leaf without consequences on transitory starch. In mature grain, overexpressed TaAMY1 was mainly located in the endosperm with high expression of TaAmy1. This is due to early developing grains having effect on starch granules from 18 days post-anthesis (DPA) and on soluble sugar accumulation from 30 DPA. While accumulation of TaAMY1 led to a high degree of damaged starch in grain, the dramatic alterations of starch visco-properties caused by the elevated levels of α-amylase essentially occurred during processing, thus suggesting a very small impact of related starch damage on grain properties. Abnormal accumulation of soluble sugar (α-gluco-oligosaccharide and sucrose) by TaAMY1 over-expression reduced the grain dormancy and enhanced abscisic acid (ABA) resistance. Germination study in the presence of α-amylase inhibitor suggested a very limited role of TaAMY1 in the early germination process and starch conversion into soluble sugars.https://www.frontiersin.org/articles/10.3389/fpls.2022.811728/fulllate maturity alpha-amylasegerminationwheatdormancystarchsprouting |
| spellingShingle | Qin Zhang Qin Zhang Jenifer Pritchard Jos Mieog Jos Mieog Keren Byrne Keren Byrne Michelle L. Colgrave Michelle L. Colgrave Ji-Rui Wang Jean-Philippe F. Ral Over-Expression of a Wheat Late Maturity Alpha-Amylase Type 1 Impact on Starch Properties During Grain Development and Germination Frontiers in Plant Science late maturity alpha-amylase germination wheat dormancy starch sprouting |
| title | Over-Expression of a Wheat Late Maturity Alpha-Amylase Type 1 Impact on Starch Properties During Grain Development and Germination |
| title_full | Over-Expression of a Wheat Late Maturity Alpha-Amylase Type 1 Impact on Starch Properties During Grain Development and Germination |
| title_fullStr | Over-Expression of a Wheat Late Maturity Alpha-Amylase Type 1 Impact on Starch Properties During Grain Development and Germination |
| title_full_unstemmed | Over-Expression of a Wheat Late Maturity Alpha-Amylase Type 1 Impact on Starch Properties During Grain Development and Germination |
| title_short | Over-Expression of a Wheat Late Maturity Alpha-Amylase Type 1 Impact on Starch Properties During Grain Development and Germination |
| title_sort | over expression of a wheat late maturity alpha amylase type 1 impact on starch properties during grain development and germination |
| topic | late maturity alpha-amylase germination wheat dormancy starch sprouting |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.811728/full |
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