Rapid detection of Staphylococcus aureus in blood culture samples using human IgG-based lateral flow assay
ABSTRACTStaphylococcus aureus is one of the most common pathogens. The conventional workflow for identifying this organism is time-consuming and takes up to several days. Therefore, we developed a colloidal gold-based lateral flow immunoassay (LFIA) using human IgG as a conjugated antibody to detect...
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2024-02-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.03046-23 |
| _version_ | 1797322822696042496 |
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| author | Arpasiri Srisrattakarn Nicha Charoensri Jeerati Prompipak Wajeeorn Ouancharee Bhanubong Saiboonjan Patcharaporn Tippayawat Aroonwadee Chanawong Lumyai Wonglakorn Ekgarak Kanwattanee Sirikan Piyapatthanakul Thitimar Masmalai Anisara Ariyapim Rinjong Promson Kendal Aroonlug Lulitanond |
| author_facet | Arpasiri Srisrattakarn Nicha Charoensri Jeerati Prompipak Wajeeorn Ouancharee Bhanubong Saiboonjan Patcharaporn Tippayawat Aroonwadee Chanawong Lumyai Wonglakorn Ekgarak Kanwattanee Sirikan Piyapatthanakul Thitimar Masmalai Anisara Ariyapim Rinjong Promson Kendal Aroonlug Lulitanond |
| author_sort | Arpasiri Srisrattakarn |
| collection | DOAJ |
| description | ABSTRACTStaphylococcus aureus is one of the most common pathogens. The conventional workflow for identifying this organism is time-consuming and takes up to several days. Therefore, we developed a colloidal gold-based lateral flow immunoassay (LFIA) using human IgG as a conjugated antibody to detect S. aureus. One hundred and thirty-eight clinical isolates, including 79 S. aureus and 59 non-S. aureus were spiked in blood samples, and incubated at 37°C for 24 h. The bacterial antigens were simply extracted before being tested by the developed LFIA strips. The results were read by the naked eye within 15 min. Conventional PCR was used as a reference method. The sensitivity and specificity of the developed LFIA were 100% (95% CI: 94.2%–100.0% and 92.4%–100.0%, respectively) in spiked blood culture samples. The detection limits of the LFIA for the purified protein A and bacterial colonies were 10−3 µg/mL and 107 CFU/mL, respectively. The performance of the LFIA testing in 221 bacterial colony isolates and 118 positive blood culture bottles from three hospitals by their medical technologists showed 98.1% (95% CI: 94.1%–99.5%) and 89.7% (95% CI: 79.3%–95.4%) sensitivity, respectively. The LFIA is a quick, easy, and sensitive method for detecting S. aureus without expensive equipment. It might have the potential for early diagnosis of routine service in low-resource laboratories, leading to a rapid and effective treatment.IMPORTANCEIn this study, we modified our previously developed lateral flow immunoassay (LFIA) test for the detection of Staphylococcus aureus by using an in-house human IgG as a conjugated antibody instead of the specific commercial antibody. It gave comparable results to the former developed-LFIA test and helped cost reduction. |
| first_indexed | 2024-03-08T05:18:51Z |
| format | Article |
| id | doaj.art-3ca681395da74287bf148f5b8fc34993 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-08T05:18:51Z |
| publishDate | 2024-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-3ca681395da74287bf148f5b8fc349932024-02-06T14:04:54ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972024-02-0112210.1128/spectrum.03046-23Rapid detection of Staphylococcus aureus in blood culture samples using human IgG-based lateral flow assayArpasiri Srisrattakarn0Nicha Charoensri1Jeerati Prompipak2Wajeeorn Ouancharee3Bhanubong Saiboonjan4Patcharaporn Tippayawat5Aroonwadee Chanawong6Lumyai Wonglakorn7Ekgarak Kanwattanee8Sirikan Piyapatthanakul9Thitimar Masmalai10Anisara Ariyapim11Rinjong Promson Kendal12Aroonlug Lulitanond13Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, ThailandCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, ThailandCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, ThailandCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, ThailandCenter for Innovation and Standard for Medical Technology and Physical Therapy, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, ThailandCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, ThailandCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, ThailandClinical Microbiology Unit, Srinagarind Hospital, Khon Kaen University, Khon Kaen, ThailandClinical Microbiology Laboratory, The Queen Sirikit National Institute of Child Health, Bangkok, ThailandClinical Microbiology Laboratory, The Queen Sirikit National Institute of Child Health, Bangkok, ThailandClinical Laboratory, Queen Sirikit Heart Center of the Northeast, Khon Kaen University, Khon Kaen, ThailandClinical Laboratory, Queen Sirikit Heart Center of the Northeast, Khon Kaen University, Khon Kaen, ThailandKhon Kaen Hospital, Khon Kaen, ThailandCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, ThailandABSTRACTStaphylococcus aureus is one of the most common pathogens. The conventional workflow for identifying this organism is time-consuming and takes up to several days. Therefore, we developed a colloidal gold-based lateral flow immunoassay (LFIA) using human IgG as a conjugated antibody to detect S. aureus. One hundred and thirty-eight clinical isolates, including 79 S. aureus and 59 non-S. aureus were spiked in blood samples, and incubated at 37°C for 24 h. The bacterial antigens were simply extracted before being tested by the developed LFIA strips. The results were read by the naked eye within 15 min. Conventional PCR was used as a reference method. The sensitivity and specificity of the developed LFIA were 100% (95% CI: 94.2%–100.0% and 92.4%–100.0%, respectively) in spiked blood culture samples. The detection limits of the LFIA for the purified protein A and bacterial colonies were 10−3 µg/mL and 107 CFU/mL, respectively. The performance of the LFIA testing in 221 bacterial colony isolates and 118 positive blood culture bottles from three hospitals by their medical technologists showed 98.1% (95% CI: 94.1%–99.5%) and 89.7% (95% CI: 79.3%–95.4%) sensitivity, respectively. The LFIA is a quick, easy, and sensitive method for detecting S. aureus without expensive equipment. It might have the potential for early diagnosis of routine service in low-resource laboratories, leading to a rapid and effective treatment.IMPORTANCEIn this study, we modified our previously developed lateral flow immunoassay (LFIA) test for the detection of Staphylococcus aureus by using an in-house human IgG as a conjugated antibody instead of the specific commercial antibody. It gave comparable results to the former developed-LFIA test and helped cost reduction.https://journals.asm.org/doi/10.1128/spectrum.03046-23lateral flow immunoassaypositive blood culture samplesrapid detectionStaphylococcus aureus |
| spellingShingle | Arpasiri Srisrattakarn Nicha Charoensri Jeerati Prompipak Wajeeorn Ouancharee Bhanubong Saiboonjan Patcharaporn Tippayawat Aroonwadee Chanawong Lumyai Wonglakorn Ekgarak Kanwattanee Sirikan Piyapatthanakul Thitimar Masmalai Anisara Ariyapim Rinjong Promson Kendal Aroonlug Lulitanond Rapid detection of Staphylococcus aureus in blood culture samples using human IgG-based lateral flow assay Microbiology Spectrum lateral flow immunoassay positive blood culture samples rapid detection Staphylococcus aureus |
| title | Rapid detection of Staphylococcus aureus in blood culture samples using human IgG-based lateral flow assay |
| title_full | Rapid detection of Staphylococcus aureus in blood culture samples using human IgG-based lateral flow assay |
| title_fullStr | Rapid detection of Staphylococcus aureus in blood culture samples using human IgG-based lateral flow assay |
| title_full_unstemmed | Rapid detection of Staphylococcus aureus in blood culture samples using human IgG-based lateral flow assay |
| title_short | Rapid detection of Staphylococcus aureus in blood culture samples using human IgG-based lateral flow assay |
| title_sort | rapid detection of staphylococcus aureus in blood culture samples using human igg based lateral flow assay |
| topic | lateral flow immunoassay positive blood culture samples rapid detection Staphylococcus aureus |
| url | https://journals.asm.org/doi/10.1128/spectrum.03046-23 |
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