Genome-Wide Identification of <i>PIN</i> and <i>PILS</i> Gene Families in <i>Areca catechu</i> and the Potential Role of <i>AcPIN6</i> in Lateral Brace Root Formation
PIN-FORMED (PIN) and PIN-LIKES (PILS) are two families of auxin transporters that control the directional cell-to-cell transport and intracellular accumulation of auxin, thereby influencing plant growth and development. Most knowledge of PINs and PILSs was obtained from the dicot model plant <i&g...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/1/33 |
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| author | Yicheng Wang Guangzhen Zhou Haifen Luo Xinyu Li Kelan Zhang Yinglang Wan |
| author_facet | Yicheng Wang Guangzhen Zhou Haifen Luo Xinyu Li Kelan Zhang Yinglang Wan |
| author_sort | Yicheng Wang |
| collection | DOAJ |
| description | PIN-FORMED (PIN) and PIN-LIKES (PILS) are two families of auxin transporters that control the directional cell-to-cell transport and intracellular accumulation of auxin, thereby influencing plant growth and development. Most knowledge of PINs and PILSs was obtained from the dicot model plant <i>Arabidopsis thaliana</i>. Here, we focus on the distribution and expression of the <i>PIN</i> and <i>PILS</i> gene families in areca palm (<i>Areca catechu</i>), a monocot tree. The whole genomic dataset of areca palm was used to identify twelve <i>AcPIN</i>s and eight <i>AcPILS</i>s, and a phylogenetic tree was constructed of <i>PINS</i> and <i>PILS</i> together with several other palm species, including the date palm (<i>Phoenix dactylifera</i>), oil palm (<i>Elaeis guineensis</i>), and coconut (<i>Cocos nucifera)</i>. We further analyzed the expression patterns of <i>AcPIN</i> and <i>AcPILS</i> in areca palm, and found that <i>AcPIN6</i> displayed an extremely high transcriptional abundance in the brace roots and was extremely stimulated in the lateral root primordium. This result implies that <i>AcPIN6</i> plays an important role in the growth and formation of brace roots, especially in lateral root initiation. We also overexpressed <i>AcPIN6</i> and <i>AcPIN6–eGFP</i> in <i>Arabidopsis</i>, and the results revealed that the PIN6 localized on the plasma membrane and affected auxin-related phenomena. Taken together, we analyzed the evolutionary relationships of <i>PINs</i> and <i>PILSs</i> in palm species, and the roles of <i>PIN6</i> in areca palm root formation. The results will improve the understanding of root system construction in large palm trees. |
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| language | English |
| last_indexed | 2024-03-09T09:42:28Z |
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| spelling | doaj.art-3cab772be34c4cf1a06628057c86ba542023-12-02T00:47:13ZengMDPI AGPlants2223-77472022-12-011213310.3390/plants12010033Genome-Wide Identification of <i>PIN</i> and <i>PILS</i> Gene Families in <i>Areca catechu</i> and the Potential Role of <i>AcPIN6</i> in Lateral Brace Root FormationYicheng Wang0Guangzhen Zhou1Haifen Luo2Xinyu Li3Kelan Zhang4Yinglang Wan5Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, Haikou 570228, ChinaHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, Haikou 570228, ChinaHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, Haikou 570228, ChinaHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, Haikou 570228, ChinaHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, Haikou 570228, ChinaHainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, Haikou 570228, ChinaPIN-FORMED (PIN) and PIN-LIKES (PILS) are two families of auxin transporters that control the directional cell-to-cell transport and intracellular accumulation of auxin, thereby influencing plant growth and development. Most knowledge of PINs and PILSs was obtained from the dicot model plant <i>Arabidopsis thaliana</i>. Here, we focus on the distribution and expression of the <i>PIN</i> and <i>PILS</i> gene families in areca palm (<i>Areca catechu</i>), a monocot tree. The whole genomic dataset of areca palm was used to identify twelve <i>AcPIN</i>s and eight <i>AcPILS</i>s, and a phylogenetic tree was constructed of <i>PINS</i> and <i>PILS</i> together with several other palm species, including the date palm (<i>Phoenix dactylifera</i>), oil palm (<i>Elaeis guineensis</i>), and coconut (<i>Cocos nucifera)</i>. We further analyzed the expression patterns of <i>AcPIN</i> and <i>AcPILS</i> in areca palm, and found that <i>AcPIN6</i> displayed an extremely high transcriptional abundance in the brace roots and was extremely stimulated in the lateral root primordium. This result implies that <i>AcPIN6</i> plays an important role in the growth and formation of brace roots, especially in lateral root initiation. We also overexpressed <i>AcPIN6</i> and <i>AcPIN6–eGFP</i> in <i>Arabidopsis</i>, and the results revealed that the PIN6 localized on the plasma membrane and affected auxin-related phenomena. Taken together, we analyzed the evolutionary relationships of <i>PINs</i> and <i>PILSs</i> in palm species, and the roles of <i>PIN6</i> in areca palm root formation. The results will improve the understanding of root system construction in large palm trees.https://www.mdpi.com/2223-7747/12/1/33<i>Areca catechu</i>PIN-FORMEDPILSbrace root |
| spellingShingle | Yicheng Wang Guangzhen Zhou Haifen Luo Xinyu Li Kelan Zhang Yinglang Wan Genome-Wide Identification of <i>PIN</i> and <i>PILS</i> Gene Families in <i>Areca catechu</i> and the Potential Role of <i>AcPIN6</i> in Lateral Brace Root Formation Plants <i>Areca catechu</i> PIN-FORMED PILS brace root |
| title | Genome-Wide Identification of <i>PIN</i> and <i>PILS</i> Gene Families in <i>Areca catechu</i> and the Potential Role of <i>AcPIN6</i> in Lateral Brace Root Formation |
| title_full | Genome-Wide Identification of <i>PIN</i> and <i>PILS</i> Gene Families in <i>Areca catechu</i> and the Potential Role of <i>AcPIN6</i> in Lateral Brace Root Formation |
| title_fullStr | Genome-Wide Identification of <i>PIN</i> and <i>PILS</i> Gene Families in <i>Areca catechu</i> and the Potential Role of <i>AcPIN6</i> in Lateral Brace Root Formation |
| title_full_unstemmed | Genome-Wide Identification of <i>PIN</i> and <i>PILS</i> Gene Families in <i>Areca catechu</i> and the Potential Role of <i>AcPIN6</i> in Lateral Brace Root Formation |
| title_short | Genome-Wide Identification of <i>PIN</i> and <i>PILS</i> Gene Families in <i>Areca catechu</i> and the Potential Role of <i>AcPIN6</i> in Lateral Brace Root Formation |
| title_sort | genome wide identification of i pin i and i pils i gene families in i areca catechu i and the potential role of i acpin6 i in lateral brace root formation |
| topic | <i>Areca catechu</i> PIN-FORMED PILS brace root |
| url | https://www.mdpi.com/2223-7747/12/1/33 |
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