Lipid Inhibitory Effect of (−)-loliolide Isolated from <i>Sargassum horneri</i> in 3T3-L1 Adipocytes: Inhibitory Mechanism of Adipose-Specific Proteins
<i>Sargassum horneri</i> (<i>S. horneri</i>) is a well-known brown seaweed widely distributed worldwide. Several biological activities of <i>S. horneri</i> have been reported. However, its effects on lipid metabolism and the underlying mechanisms remain elusive. I...
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MDPI AG
2021-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/2/96 |
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| author | Hyo-Geun Lee Hyun-Soo Kim Jun-Geon Je Jin Hwang K. K. Asanka Sanjeewa Dae-Sung Lee Kyung-Mo Song Yun-Sang Choi Min-Cheol Kang You-Jin Jeon |
| author_facet | Hyo-Geun Lee Hyun-Soo Kim Jun-Geon Je Jin Hwang K. K. Asanka Sanjeewa Dae-Sung Lee Kyung-Mo Song Yun-Sang Choi Min-Cheol Kang You-Jin Jeon |
| author_sort | Hyo-Geun Lee |
| collection | DOAJ |
| description | <i>Sargassum horneri</i> (<i>S. horneri</i>) is a well-known brown seaweed widely distributed worldwide. Several biological activities of <i>S. horneri</i> have been reported. However, its effects on lipid metabolism and the underlying mechanisms remain elusive. In the present study, we examined the inhibitory effect of the active compound “(−)-loliolide ((6S,7aR)-6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydro-1-benzofuran-2(4H)-one (HTT))” from <i>S. horneri</i> extract on lipid accumulation in differentiated adipocytes. MTT assays demonstrated that (−)-loliolide is not toxic to 3T3-L1 adipocytes in a range of concentrations. (−)-loliolide significantly reduced intracellular lipid accumulation in the differentiated phase of 3T3-L1 adipocytes as shown by Oil Red O staining. Western blot analysis revealed that (−)-loliolide increased the expression of lipolytic protein phospho-hormone-sensitive lipase (p-HSL) and thermogenic protein peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1). Additionally, (−)-loliolide decreased expression of adipogenic and lipogenic proteins, including sterol regulatory element-binding protein-1 (SREBP-1), peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT/enhancer-binding protein-α (C/EBP-α), and fatty acid-binding protein 4 (FABP4) in 3T3-L1 adipocytes. These results indicate that (−)-loliolide from <i>S. horneri</i> could suppress lipid accumulation via regulation of antiadipogenic and prolipolytic mechanisms in 3T3-L1 cells. Considering the multifunctional effect of (−)-loliolide, it can be useful as a lipid-lowering agent in the management of patients who suffer from obesity. |
| first_indexed | 2024-03-09T05:09:53Z |
| format | Article |
| id | doaj.art-3cabe0a7ef6443faaec20ad1d3922caf |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T05:09:53Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-3cabe0a7ef6443faaec20ad1d3922caf2023-12-03T12:50:02ZengMDPI AGMarine Drugs1660-33972021-02-011929610.3390/md19020096Lipid Inhibitory Effect of (−)-loliolide Isolated from <i>Sargassum horneri</i> in 3T3-L1 Adipocytes: Inhibitory Mechanism of Adipose-Specific ProteinsHyo-Geun Lee0Hyun-Soo Kim1Jun-Geon Je2Jin Hwang3K. K. Asanka Sanjeewa4Dae-Sung Lee5Kyung-Mo Song6Yun-Sang Choi7Min-Cheol Kang8You-Jin Jeon9Department of Marine Life Science, Jeju National University, Jeju 63243, KoreaMarine Biodiversity Institute of Korea, 75, Jangsan-ro 101-gil, Janghang-eup, Seocheon 33362, KoreaDepartment of Marine Life Science, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Science, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Science, Jeju National University, Jeju 63243, KoreaMarine Biodiversity Institute of Korea, 75, Jangsan-ro 101-gil, Janghang-eup, Seocheon 33362, KoreaResearch Group of Food Processing, Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju 55365, KoreaResearch Group of Food Processing, Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju 55365, KoreaResearch Group of Food Processing, Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju 55365, KoreaDepartment of Marine Life Science, Jeju National University, Jeju 63243, Korea<i>Sargassum horneri</i> (<i>S. horneri</i>) is a well-known brown seaweed widely distributed worldwide. Several biological activities of <i>S. horneri</i> have been reported. However, its effects on lipid metabolism and the underlying mechanisms remain elusive. In the present study, we examined the inhibitory effect of the active compound “(−)-loliolide ((6S,7aR)-6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydro-1-benzofuran-2(4H)-one (HTT))” from <i>S. horneri</i> extract on lipid accumulation in differentiated adipocytes. MTT assays demonstrated that (−)-loliolide is not toxic to 3T3-L1 adipocytes in a range of concentrations. (−)-loliolide significantly reduced intracellular lipid accumulation in the differentiated phase of 3T3-L1 adipocytes as shown by Oil Red O staining. Western blot analysis revealed that (−)-loliolide increased the expression of lipolytic protein phospho-hormone-sensitive lipase (p-HSL) and thermogenic protein peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1). Additionally, (−)-loliolide decreased expression of adipogenic and lipogenic proteins, including sterol regulatory element-binding protein-1 (SREBP-1), peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT/enhancer-binding protein-α (C/EBP-α), and fatty acid-binding protein 4 (FABP4) in 3T3-L1 adipocytes. These results indicate that (−)-loliolide from <i>S. horneri</i> could suppress lipid accumulation via regulation of antiadipogenic and prolipolytic mechanisms in 3T3-L1 cells. Considering the multifunctional effect of (−)-loliolide, it can be useful as a lipid-lowering agent in the management of patients who suffer from obesity.https://www.mdpi.com/1660-3397/19/2/96<i>Sargassum horneri</i>(−)-loliolidelipid metabolism3T3-L1 adipocytes |
| spellingShingle | Hyo-Geun Lee Hyun-Soo Kim Jun-Geon Je Jin Hwang K. K. Asanka Sanjeewa Dae-Sung Lee Kyung-Mo Song Yun-Sang Choi Min-Cheol Kang You-Jin Jeon Lipid Inhibitory Effect of (−)-loliolide Isolated from <i>Sargassum horneri</i> in 3T3-L1 Adipocytes: Inhibitory Mechanism of Adipose-Specific Proteins Marine Drugs <i>Sargassum horneri</i> (−)-loliolide lipid metabolism 3T3-L1 adipocytes |
| title | Lipid Inhibitory Effect of (−)-loliolide Isolated from <i>Sargassum horneri</i> in 3T3-L1 Adipocytes: Inhibitory Mechanism of Adipose-Specific Proteins |
| title_full | Lipid Inhibitory Effect of (−)-loliolide Isolated from <i>Sargassum horneri</i> in 3T3-L1 Adipocytes: Inhibitory Mechanism of Adipose-Specific Proteins |
| title_fullStr | Lipid Inhibitory Effect of (−)-loliolide Isolated from <i>Sargassum horneri</i> in 3T3-L1 Adipocytes: Inhibitory Mechanism of Adipose-Specific Proteins |
| title_full_unstemmed | Lipid Inhibitory Effect of (−)-loliolide Isolated from <i>Sargassum horneri</i> in 3T3-L1 Adipocytes: Inhibitory Mechanism of Adipose-Specific Proteins |
| title_short | Lipid Inhibitory Effect of (−)-loliolide Isolated from <i>Sargassum horneri</i> in 3T3-L1 Adipocytes: Inhibitory Mechanism of Adipose-Specific Proteins |
| title_sort | lipid inhibitory effect of loliolide isolated from i sargassum horneri i in 3t3 l1 adipocytes inhibitory mechanism of adipose specific proteins |
| topic | <i>Sargassum horneri</i> (−)-loliolide lipid metabolism 3T3-L1 adipocytes |
| url | https://www.mdpi.com/1660-3397/19/2/96 |
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