Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells

ABSTRACT The objective of this study was to investigate the effects of recombinant adiponectin on chicken liver cells. The full-length chicken adiponectin gene was amplified by PCR and cloned into the vector pET-32a, followed by the transformation of the vector into Escherichia coli BL21. SDS-PAGE w...

Full description

Bibliographic Details
Main Authors: Yi-Ru Zhuang, Yuan-Yu Lin
Format: Article
Language:English
Published: Sociedade Brasileira de Zootecnia 2022-11-01
Series:Revista Brasileira de Zootecnia
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982022000100409&tlng=en
_version_ 1797987044151001088
author Yi-Ru Zhuang
Yuan-Yu Lin
author_facet Yi-Ru Zhuang
Yuan-Yu Lin
author_sort Yi-Ru Zhuang
collection DOAJ
description ABSTRACT The objective of this study was to investigate the effects of recombinant adiponectin on chicken liver cells. The full-length chicken adiponectin gene was amplified by PCR and cloned into the vector pET-32a, followed by the transformation of the vector into Escherichia coli BL21. SDS-PAGE was used to detect and analyze the purity of the expressed recombinant protein. Induction was performed with 1 mM IPTG at 30 °C for 3 h, and the recombinant thioredoxin–adiponectin fusion protein was purified using Ni-NTA affinity chromatography. Chicken adiponectin was successfully expressed and purified in a bacterial system. In addition, the chicken recombinant adiponectin demonstrated that it ameliorates palmitic acid- and oleic acid-induced adipogenesis, in which an increase in β-oxidation and a decrease in lipogenesis-related genes may be involved. In summary, chicken recombinant adiponectin enhances fatty acid metabolism in LMH cells.
first_indexed 2024-04-11T07:42:44Z
format Article
id doaj.art-3cad89049cab49599c170e2f72a86a46
institution Directory Open Access Journal
issn 1806-9290
language English
last_indexed 2024-04-11T07:42:44Z
publishDate 2022-11-01
publisher Sociedade Brasileira de Zootecnia
record_format Article
series Revista Brasileira de Zootecnia
spelling doaj.art-3cad89049cab49599c170e2f72a86a462022-12-22T04:36:28ZengSociedade Brasileira de ZootecniaRevista Brasileira de Zootecnia1806-92902022-11-015110.37496/rbz5120220087Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cellsYi-Ru Zhuanghttps://orcid.org/0000-0003-2952-6922Yuan-Yu Linhttps://orcid.org/0000-0002-3397-3218ABSTRACT The objective of this study was to investigate the effects of recombinant adiponectin on chicken liver cells. The full-length chicken adiponectin gene was amplified by PCR and cloned into the vector pET-32a, followed by the transformation of the vector into Escherichia coli BL21. SDS-PAGE was used to detect and analyze the purity of the expressed recombinant protein. Induction was performed with 1 mM IPTG at 30 °C for 3 h, and the recombinant thioredoxin–adiponectin fusion protein was purified using Ni-NTA affinity chromatography. Chicken adiponectin was successfully expressed and purified in a bacterial system. In addition, the chicken recombinant adiponectin demonstrated that it ameliorates palmitic acid- and oleic acid-induced adipogenesis, in which an increase in β-oxidation and a decrease in lipogenesis-related genes may be involved. In summary, chicken recombinant adiponectin enhances fatty acid metabolism in LMH cells.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982022000100409&tlng=enchickenfatty acid metabolismrecombinant protein
spellingShingle Yi-Ru Zhuang
Yuan-Yu Lin
Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells
Revista Brasileira de Zootecnia
chicken
fatty acid metabolism
recombinant protein
title Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells
title_full Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells
title_fullStr Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells
title_full_unstemmed Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells
title_short Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells
title_sort chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid and palmitic acid treated lmh cells
topic chicken
fatty acid metabolism
recombinant protein
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982022000100409&tlng=en
work_keys_str_mv AT yiruzhuang chickenrecombinantadiponectinenhancesfattyacidmetabolisminoleicacidandpalmiticacidtreatedlmhcells
AT yuanyulin chickenrecombinantadiponectinenhancesfattyacidmetabolisminoleicacidandpalmiticacidtreatedlmhcells