Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells
ABSTRACT The objective of this study was to investigate the effects of recombinant adiponectin on chicken liver cells. The full-length chicken adiponectin gene was amplified by PCR and cloned into the vector pET-32a, followed by the transformation of the vector into Escherichia coli BL21. SDS-PAGE w...
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Format: | Article |
Language: | English |
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Sociedade Brasileira de Zootecnia
2022-11-01
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Series: | Revista Brasileira de Zootecnia |
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982022000100409&tlng=en |
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author | Yi-Ru Zhuang Yuan-Yu Lin |
author_facet | Yi-Ru Zhuang Yuan-Yu Lin |
author_sort | Yi-Ru Zhuang |
collection | DOAJ |
description | ABSTRACT The objective of this study was to investigate the effects of recombinant adiponectin on chicken liver cells. The full-length chicken adiponectin gene was amplified by PCR and cloned into the vector pET-32a, followed by the transformation of the vector into Escherichia coli BL21. SDS-PAGE was used to detect and analyze the purity of the expressed recombinant protein. Induction was performed with 1 mM IPTG at 30 °C for 3 h, and the recombinant thioredoxin–adiponectin fusion protein was purified using Ni-NTA affinity chromatography. Chicken adiponectin was successfully expressed and purified in a bacterial system. In addition, the chicken recombinant adiponectin demonstrated that it ameliorates palmitic acid- and oleic acid-induced adipogenesis, in which an increase in β-oxidation and a decrease in lipogenesis-related genes may be involved. In summary, chicken recombinant adiponectin enhances fatty acid metabolism in LMH cells. |
first_indexed | 2024-04-11T07:42:44Z |
format | Article |
id | doaj.art-3cad89049cab49599c170e2f72a86a46 |
institution | Directory Open Access Journal |
issn | 1806-9290 |
language | English |
last_indexed | 2024-04-11T07:42:44Z |
publishDate | 2022-11-01 |
publisher | Sociedade Brasileira de Zootecnia |
record_format | Article |
series | Revista Brasileira de Zootecnia |
spelling | doaj.art-3cad89049cab49599c170e2f72a86a462022-12-22T04:36:28ZengSociedade Brasileira de ZootecniaRevista Brasileira de Zootecnia1806-92902022-11-015110.37496/rbz5120220087Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cellsYi-Ru Zhuanghttps://orcid.org/0000-0003-2952-6922Yuan-Yu Linhttps://orcid.org/0000-0002-3397-3218ABSTRACT The objective of this study was to investigate the effects of recombinant adiponectin on chicken liver cells. The full-length chicken adiponectin gene was amplified by PCR and cloned into the vector pET-32a, followed by the transformation of the vector into Escherichia coli BL21. SDS-PAGE was used to detect and analyze the purity of the expressed recombinant protein. Induction was performed with 1 mM IPTG at 30 °C for 3 h, and the recombinant thioredoxin–adiponectin fusion protein was purified using Ni-NTA affinity chromatography. Chicken adiponectin was successfully expressed and purified in a bacterial system. In addition, the chicken recombinant adiponectin demonstrated that it ameliorates palmitic acid- and oleic acid-induced adipogenesis, in which an increase in β-oxidation and a decrease in lipogenesis-related genes may be involved. In summary, chicken recombinant adiponectin enhances fatty acid metabolism in LMH cells.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982022000100409&tlng=enchickenfatty acid metabolismrecombinant protein |
spellingShingle | Yi-Ru Zhuang Yuan-Yu Lin Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells Revista Brasileira de Zootecnia chicken fatty acid metabolism recombinant protein |
title | Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells |
title_full | Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells |
title_fullStr | Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells |
title_full_unstemmed | Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells |
title_short | Chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid- and palmitic acid-treated LMH cells |
title_sort | chicken recombinant adiponectin enhances fatty acid metabolism in oleic acid and palmitic acid treated lmh cells |
topic | chicken fatty acid metabolism recombinant protein |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982022000100409&tlng=en |
work_keys_str_mv | AT yiruzhuang chickenrecombinantadiponectinenhancesfattyacidmetabolisminoleicacidandpalmiticacidtreatedlmhcells AT yuanyulin chickenrecombinantadiponectinenhancesfattyacidmetabolisminoleicacidandpalmiticacidtreatedlmhcells |