Immunologic patterns associated with cure in human American cutaneous leishmaniasis
Patients with American cutaneous leishmaniasis were studied before therapy (active lesion) and at the end of therapy (cured patients). Assays of lymphocyte proliferative responses of peripheral blood mononuclear cells induced in vitro by Leishmania braziliensis promastigote antigens (Lb) were perfor...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
1998-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100019 |
| _version_ | 1811266526436655104 |
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| author | Coutinho S.G. Da-Cruz A.M. Bertho A.L. Santiago M.A. De-Luca P. |
| author_facet | Coutinho S.G. Da-Cruz A.M. Bertho A.L. Santiago M.A. De-Luca P. |
| author_sort | Coutinho S.G. |
| collection | DOAJ |
| description | Patients with American cutaneous leishmaniasis were studied before therapy (active lesion) and at the end of therapy (cured patients). Assays of lymphocyte proliferative responses of peripheral blood mononuclear cells induced in vitro by Leishmania braziliensis promastigote antigens (Lb) were performed. Antigen-stimulated cells were harvested for CD4 and CD8 phenotype analysis and the levels of gamma interferon (IFN-<FONT FACE="Symbol">g</font>) and interleukin 4 (IL-4) produced were also determined in the culture supernatants. Two different patterns of Lb-induced T cell responses were observed: a) predominance of responding CD4+ cells and mixed type 1 and type 2 cytokine production (IFN-<FONT FACE="Symbol">g</font> and IL-4) during the active disease, and b) similar proportions of responding CD4+ and CD8+ cells, and type 1 cytokine production (presence of IFN-<FONT FACE="Symbol">g</font> and very low IL-4) at the end of therapy (healed lesions). This last pattern is probably associated with a beneficial T cell response |
| first_indexed | 2024-04-12T20:44:46Z |
| format | Article |
| id | doaj.art-3cae0c441a7e43bea6b30b1babe40493 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 0034-7310 |
| language | English |
| last_indexed | 2024-04-12T20:44:46Z |
| publishDate | 1998-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-3cae0c441a7e43bea6b30b1babe404932022-12-22T03:17:18ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X0034-73101998-01-01311139142Immunologic patterns associated with cure in human American cutaneous leishmaniasisCoutinho S.G.Da-Cruz A.M.Bertho A.L.Santiago M.A.De-Luca P.Patients with American cutaneous leishmaniasis were studied before therapy (active lesion) and at the end of therapy (cured patients). Assays of lymphocyte proliferative responses of peripheral blood mononuclear cells induced in vitro by Leishmania braziliensis promastigote antigens (Lb) were performed. Antigen-stimulated cells were harvested for CD4 and CD8 phenotype analysis and the levels of gamma interferon (IFN-<FONT FACE="Symbol">g</font>) and interleukin 4 (IL-4) produced were also determined in the culture supernatants. Two different patterns of Lb-induced T cell responses were observed: a) predominance of responding CD4+ cells and mixed type 1 and type 2 cytokine production (IFN-<FONT FACE="Symbol">g</font> and IL-4) during the active disease, and b) similar proportions of responding CD4+ and CD8+ cells, and type 1 cytokine production (presence of IFN-<FONT FACE="Symbol">g</font> and very low IL-4) at the end of therapy (healed lesions). This last pattern is probably associated with a beneficial T cell responsehttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100019human cutaneous leishmaniasisLeishmania braziliensisCD4+ and CD8+ cellsIFN-<FONT FACE=Symbol>g</font>IL-4 |
| spellingShingle | Coutinho S.G. Da-Cruz A.M. Bertho A.L. Santiago M.A. De-Luca P. Immunologic patterns associated with cure in human American cutaneous leishmaniasis Brazilian Journal of Medical and Biological Research human cutaneous leishmaniasis Leishmania braziliensis CD4+ and CD8+ cells IFN-<FONT FACE=Symbol>g</font> IL-4 |
| title | Immunologic patterns associated with cure in human American cutaneous leishmaniasis |
| title_full | Immunologic patterns associated with cure in human American cutaneous leishmaniasis |
| title_fullStr | Immunologic patterns associated with cure in human American cutaneous leishmaniasis |
| title_full_unstemmed | Immunologic patterns associated with cure in human American cutaneous leishmaniasis |
| title_short | Immunologic patterns associated with cure in human American cutaneous leishmaniasis |
| title_sort | immunologic patterns associated with cure in human american cutaneous leishmaniasis |
| topic | human cutaneous leishmaniasis Leishmania braziliensis CD4+ and CD8+ cells IFN-<FONT FACE=Symbol>g</font> IL-4 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100019 |
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