Simultaneous Determination of 9 Biogenic Amines in Larimichthys polyactis by UHPLC-MS/MS
To rapidly determine 9 biogenic amines including spermine, spermidine, putrescine, cadaverine, histamine, tryptamine, octopamine, tyramine and β-phenethylamine in aquatic products, the ultra high-performance liquid chromatography-tandem mass spectrometry method was established with Larimichthys poly...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-03-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022040183 |
| _version_ | 1811162796392448000 |
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| author | Zhenling LUO Haibo GAO Ting YANG Yu FU |
| author_facet | Zhenling LUO Haibo GAO Ting YANG Yu FU |
| author_sort | Zhenling LUO |
| collection | DOAJ |
| description | To rapidly determine 9 biogenic amines including spermine, spermidine, putrescine, cadaverine, histamine, tryptamine, octopamine, tyramine and β-phenethylamine in aquatic products, the ultra high-performance liquid chromatography-tandem mass spectrometry method was established with Larimichthys polyactis using as matrix sample, and mobile phase and extract solvents were optimized. Matrix sample was extracted by 0.1% trichloroacetic acid (TCA) in duplicate, the supernatant was combined and filtered by 0.22 μm PTFE filters, and separated by HSS T3 column in 4 min. The mobile phase A was acetonitrile containing 0.1% formic acid, while mobile phase B was 10 mmol/L ammonium formate containing 0.2% formic acid. Nine biogenic amines were divided into three groups based on their different response intensity, with a concentration range of 1 to 40 ng/mL (tyramine and β-phenethylamine), 5 to 200 ng/mL (putrescine, cadaverine, histamine, tryptamine and octopamine), and 25 to 1000 ng/mL (spermine and spermidine). Their method quantification limits (LOQs) were 0.01, 0.05 and 0.25 mg/kg, respectively. The recovery rate of low, medium and high concentrations was 81.7%~111.2%, 80.0%~110.3% and 80.0%~99.0%, respectively, with the corresponding degree of precision (n=6) of 1.7%~7.3%, 1.3%~9.1% and 0.7%~4.6%. This method is simple, fast and with good sensitivity, recovery and precision, which is suitable for the determination of biogenic amines in Larimichthys polyactis. |
| first_indexed | 2024-04-10T06:34:56Z |
| format | Article |
| id | doaj.art-3cb4b5fcd5c54ec985989aed7d093bfd |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-10T06:34:56Z |
| publishDate | 2023-03-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-3cb4b5fcd5c54ec985989aed7d093bfd2023-03-01T01:21:35ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-03-0144525125710.13386/j.issn1002-0306.20220401832022040183-5Simultaneous Determination of 9 Biogenic Amines in Larimichthys polyactis by UHPLC-MS/MSZhenling LUO0Haibo GAO1Ting YANG2Yu FU3Taizhou Food Inspection Centre, Taizhou 318100, ChinaTaizhou Food Inspection Centre, Taizhou 318100, ChinaTaizhou Food Inspection Centre, Taizhou 318100, ChinaCollege of Food Science, Southwest University, Chongqing 400715, ChinaTo rapidly determine 9 biogenic amines including spermine, spermidine, putrescine, cadaverine, histamine, tryptamine, octopamine, tyramine and β-phenethylamine in aquatic products, the ultra high-performance liquid chromatography-tandem mass spectrometry method was established with Larimichthys polyactis using as matrix sample, and mobile phase and extract solvents were optimized. Matrix sample was extracted by 0.1% trichloroacetic acid (TCA) in duplicate, the supernatant was combined and filtered by 0.22 μm PTFE filters, and separated by HSS T3 column in 4 min. The mobile phase A was acetonitrile containing 0.1% formic acid, while mobile phase B was 10 mmol/L ammonium formate containing 0.2% formic acid. Nine biogenic amines were divided into three groups based on their different response intensity, with a concentration range of 1 to 40 ng/mL (tyramine and β-phenethylamine), 5 to 200 ng/mL (putrescine, cadaverine, histamine, tryptamine and octopamine), and 25 to 1000 ng/mL (spermine and spermidine). Their method quantification limits (LOQs) were 0.01, 0.05 and 0.25 mg/kg, respectively. The recovery rate of low, medium and high concentrations was 81.7%~111.2%, 80.0%~110.3% and 80.0%~99.0%, respectively, with the corresponding degree of precision (n=6) of 1.7%~7.3%, 1.3%~9.1% and 0.7%~4.6%. This method is simple, fast and with good sensitivity, recovery and precision, which is suitable for the determination of biogenic amines in Larimichthys polyactis.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022040183biogenic aminesultra high-performance liquid chromatography-tandem mass spectrometry (uhplc-ms/ms)larimichthys polyactis |
| spellingShingle | Zhenling LUO Haibo GAO Ting YANG Yu FU Simultaneous Determination of 9 Biogenic Amines in Larimichthys polyactis by UHPLC-MS/MS Shipin gongye ke-ji biogenic amines ultra high-performance liquid chromatography-tandem mass spectrometry (uhplc-ms/ms) larimichthys polyactis |
| title | Simultaneous Determination of 9 Biogenic Amines in Larimichthys polyactis by UHPLC-MS/MS |
| title_full | Simultaneous Determination of 9 Biogenic Amines in Larimichthys polyactis by UHPLC-MS/MS |
| title_fullStr | Simultaneous Determination of 9 Biogenic Amines in Larimichthys polyactis by UHPLC-MS/MS |
| title_full_unstemmed | Simultaneous Determination of 9 Biogenic Amines in Larimichthys polyactis by UHPLC-MS/MS |
| title_short | Simultaneous Determination of 9 Biogenic Amines in Larimichthys polyactis by UHPLC-MS/MS |
| title_sort | simultaneous determination of 9 biogenic amines in larimichthys polyactis by uhplc ms ms |
| topic | biogenic amines ultra high-performance liquid chromatography-tandem mass spectrometry (uhplc-ms/ms) larimichthys polyactis |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022040183 |
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