Molecular Identification of Lactic Acid Bacteria Approach to Sustainable food Security
Background: Dadiah is a traditional dish from West Sumatra made from buffalo milk, which is fermented in bamboo tubes and left at room temperature for ±2 days. Dadiah is included in the staple food category because it contains Lactic Acid Bacteria (LAB) which has the potential to be a probiotic. Thi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2021-11-01
|
| Series: | Journal of Public Health Research |
| Subjects: | |
| Online Access: | https://www.jphres.org/index.php/jphres/article/view/2508 |
| _version_ | 1797971544342790144 |
|---|---|
| author | Dessy Abdullah Sandeep Poddar Ramesh Prasath Rai Endang Purwati Nadia Purnama Dewi Yudha Endra Pratama |
| author_facet | Dessy Abdullah Sandeep Poddar Ramesh Prasath Rai Endang Purwati Nadia Purnama Dewi Yudha Endra Pratama |
| author_sort | Dessy Abdullah |
| collection | DOAJ |
| description | Background: Dadiah is a traditional dish from West Sumatra made from buffalo milk, which is fermented in bamboo tubes and left at room temperature for ±2 days. Dadiah is included in the staple food category because it contains Lactic Acid Bacteria (LAB) which has the potential to be a probiotic. This study aims to determine the identification and characterization of LAB from Dadiah from Halaban, Kab. Fifty Cities, West Sumatra.
Design and Methods: A survey method was used in this research with a descriptive analysis, Antimicrobial activity testing was done with bacteria Escherichia coli O157, Staphylococcus aureus, Listeria monocytogenes, and Listeria innocua. Molecular identification was done using the 16S rRNA gene.
Results: Probiotic candidate test with the best results in testing for resistance to stomach acid at pH3 with the viability of 65.98%, bile salt resistance 0.3%, viability of 54.90% from 2DA isolates. Antimicrobial activity with the best clear zone area results was obtained in 2DA isolates with Escherichia coli O157 test bacteria of 21.16 mm, Staphylococcus aureus with a clear zone area of 23.17 mm, Listeria innocua of 19.24 mm and Listeria monocytogenes with a clear zone area 18.23 mm in 4DA isolate, LAB identification using 16S sRNA gene, results of running PCR base length 1419bp.
Conclusions: Phylogenetic analysis shows that Dadiah of Limapuluh Kota Regency is a kin to Lactobacillus plantarum. The superiority of identification technology by using 16S rRNA gene only can be conducted if the nucleotide sequence information of the targeted bacteria is known beforehand. |
| first_indexed | 2024-04-11T03:34:19Z |
| format | Article |
| id | doaj.art-3cb659a76d784e5f96b72ed19bb02e3f |
| institution | Directory Open Access Journal |
| issn | 2279-9028 2279-9036 |
| language | English |
| last_indexed | 2024-04-11T03:34:19Z |
| publishDate | 2021-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Public Health Research |
| spelling | doaj.art-3cb659a76d784e5f96b72ed19bb02e3f2023-01-02T05:37:14ZengSAGE PublishingJournal of Public Health Research2279-90282279-90362021-11-0110.4081/jphr.2021.2508Molecular Identification of Lactic Acid Bacteria Approach to Sustainable food SecurityDessy Abdullah0Sandeep Poddar1Ramesh Prasath Rai2Endang Purwati3Nadia Purnama Dewi4Yudha Endra Pratama5Research Scholar, Lincoln University College, Wisma Lincoln, No, 12-18, SS 6/12, Off Jalan Perbandaran,, 47301 Petaling Jaya, Selangor D. E, Malaysia Lecturer, Medical Faculty, Baiturrahmah University Padang, West Sumatera, IndonesiaDeputy Vice Chancellor of Research, Lincoln University CollegeLincoln University College, Wisma Lincoln, No, 12-18, SS 6/12, Off Jalan Perbandaran, 47301 Petaling Jaya, Selangor, MalaysiaFaculty of Animal Science, University of Andalas, Padang, West Sumatera, IndonesiaResearch Scholar, Lincoln University College, Wisma Lincoln, No, 12-18, SS 6/12, Off Jalan Perbandaran,, 47301 Petaling Jaya, Selangor, Malaysia Lecturer, Medical Faculty, Baiturrahmah University Padang, West Sumatera, IndonesiaDoctoral Program, Faculty of Animal Science, University of Andalas, Padang, IndonesiaBackground: Dadiah is a traditional dish from West Sumatra made from buffalo milk, which is fermented in bamboo tubes and left at room temperature for ±2 days. Dadiah is included in the staple food category because it contains Lactic Acid Bacteria (LAB) which has the potential to be a probiotic. This study aims to determine the identification and characterization of LAB from Dadiah from Halaban, Kab. Fifty Cities, West Sumatra. Design and Methods: A survey method was used in this research with a descriptive analysis, Antimicrobial activity testing was done with bacteria Escherichia coli O157, Staphylococcus aureus, Listeria monocytogenes, and Listeria innocua. Molecular identification was done using the 16S rRNA gene. Results: Probiotic candidate test with the best results in testing for resistance to stomach acid at pH3 with the viability of 65.98%, bile salt resistance 0.3%, viability of 54.90% from 2DA isolates. Antimicrobial activity with the best clear zone area results was obtained in 2DA isolates with Escherichia coli O157 test bacteria of 21.16 mm, Staphylococcus aureus with a clear zone area of 23.17 mm, Listeria innocua of 19.24 mm and Listeria monocytogenes with a clear zone area 18.23 mm in 4DA isolate, LAB identification using 16S sRNA gene, results of running PCR base length 1419bp. Conclusions: Phylogenetic analysis shows that Dadiah of Limapuluh Kota Regency is a kin to Lactobacillus plantarum. The superiority of identification technology by using 16S rRNA gene only can be conducted if the nucleotide sequence information of the targeted bacteria is known beforehand.https://www.jphres.org/index.php/jphres/article/view/2508DadiahLactobacillus plantarumLactic Acid BacteriaProbiotics |
| spellingShingle | Dessy Abdullah Sandeep Poddar Ramesh Prasath Rai Endang Purwati Nadia Purnama Dewi Yudha Endra Pratama Molecular Identification of Lactic Acid Bacteria Approach to Sustainable food Security Journal of Public Health Research Dadiah Lactobacillus plantarum Lactic Acid Bacteria Probiotics |
| title | Molecular Identification of Lactic Acid Bacteria Approach to Sustainable food Security |
| title_full | Molecular Identification of Lactic Acid Bacteria Approach to Sustainable food Security |
| title_fullStr | Molecular Identification of Lactic Acid Bacteria Approach to Sustainable food Security |
| title_full_unstemmed | Molecular Identification of Lactic Acid Bacteria Approach to Sustainable food Security |
| title_short | Molecular Identification of Lactic Acid Bacteria Approach to Sustainable food Security |
| title_sort | molecular identification of lactic acid bacteria approach to sustainable food security |
| topic | Dadiah Lactobacillus plantarum Lactic Acid Bacteria Probiotics |
| url | https://www.jphres.org/index.php/jphres/article/view/2508 |
| work_keys_str_mv | AT dessyabdullah molecularidentificationoflacticacidbacteriaapproachtosustainablefoodsecurity AT sandeeppoddar molecularidentificationoflacticacidbacteriaapproachtosustainablefoodsecurity AT rameshprasathrai molecularidentificationoflacticacidbacteriaapproachtosustainablefoodsecurity AT endangpurwati molecularidentificationoflacticacidbacteriaapproachtosustainablefoodsecurity AT nadiapurnamadewi molecularidentificationoflacticacidbacteriaapproachtosustainablefoodsecurity AT yudhaendrapratama molecularidentificationoflacticacidbacteriaapproachtosustainablefoodsecurity |