ICESsuHN105, a Novel Multiple Antibiotic Resistant ICE in Streptococcus suis Serotype 5 Strain HN105
Streptococcussuis serotype 5, an emerging zoonosis bacterial pathogen, has been isolated from infections in both pigs and humans. In this study, we sequenced the first complete genome of a virulent, multidrug-resistant SS5 strain HN105. The strain HN105 displayed enhanced pathogenicity in zebrafish...
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| Format: | Article |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00274/full |
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| author | Yinchu Zhu Yinchu Zhu Yinchu Zhu Yue Zhang Yue Zhang Yue Zhang Jiale Ma Jiale Ma Jiale Ma Wenyang Dong Wenyang Dong Wenyang Dong Xiaojun Zhong Xiaojun Zhong Xiaojun Zhong Zihao Pan Zihao Pan Zihao Pan Huochun Yao Huochun Yao Huochun Yao |
| author_facet | Yinchu Zhu Yinchu Zhu Yinchu Zhu Yue Zhang Yue Zhang Yue Zhang Jiale Ma Jiale Ma Jiale Ma Wenyang Dong Wenyang Dong Wenyang Dong Xiaojun Zhong Xiaojun Zhong Xiaojun Zhong Zihao Pan Zihao Pan Zihao Pan Huochun Yao Huochun Yao Huochun Yao |
| author_sort | Yinchu Zhu |
| collection | DOAJ |
| description | Streptococcussuis serotype 5, an emerging zoonosis bacterial pathogen, has been isolated from infections in both pigs and humans. In this study, we sequenced the first complete genome of a virulent, multidrug-resistant SS5 strain HN105. The strain HN105 displayed enhanced pathogenicity in zebrafish and BABL/c mouse infection models. Comparative genome analysis identified a novel 80K integrative conjugative element (ICE), ICESsuHN105, as required for the multidrug resistance phenotype. Six corresponding antibiotic resistance genes in this ICE were identified, namely tet (O), tet (M), erm (two copies), aph, and spc. Phylogenetic analysis classified the element as a homolog of the ICESa2603 family, containing the typical family backbone and insertion DNA. DNA hybrids mediated by natural transformation between HN105 and ZY05719 verified the antibiotic resistant genes of ICESsuHN105 that could be transferred successfully, while they were dispersedly inserted with a single gene in different genomic locations of ZY05719(HN105) transformants. To further identify the horizontal transfer of ICESsuHN105 as a whole mobile genetic element, a circular intermediate form of ICESsuHN105 was detected by PCR. However, the effective conjugation using serotype 2 S. suis as recipients was not observed in current assays in vitro. Further studies confirmed the presence of the complete lantibiotic locus encoded in ICESsuHN105 that effectively inhibits the growth of other streptococci. In summary, this study demonstrated the presence of antibiotic resistance genes in ICE that are able to transfer between different clinical isolates and adapt to a broader range of Streptococcus serotype or species. |
| first_indexed | 2024-12-13T05:09:33Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T05:09:33Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj.art-3cb87013a1c64af79e89012e5f5d29c02022-12-21T23:58:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-02-011010.3389/fmicb.2019.00274429596ICESsuHN105, a Novel Multiple Antibiotic Resistant ICE in Streptococcus suis Serotype 5 Strain HN105Yinchu Zhu0Yinchu Zhu1Yinchu Zhu2Yue Zhang3Yue Zhang4Yue Zhang5Jiale Ma6Jiale Ma7Jiale Ma8Wenyang Dong9Wenyang Dong10Wenyang Dong11Xiaojun Zhong12Xiaojun Zhong13Xiaojun Zhong14Zihao Pan15Zihao Pan16Zihao Pan17Huochun Yao18Huochun Yao19Huochun Yao20College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, Nanjing, ChinaKey Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, Nanjing, ChinaKey Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, Nanjing, ChinaKey Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, Nanjing, ChinaKey Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, Nanjing, ChinaKey Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, Nanjing, ChinaKey Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, Nanjing, ChinaKey Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, ChinaStreptococcussuis serotype 5, an emerging zoonosis bacterial pathogen, has been isolated from infections in both pigs and humans. In this study, we sequenced the first complete genome of a virulent, multidrug-resistant SS5 strain HN105. The strain HN105 displayed enhanced pathogenicity in zebrafish and BABL/c mouse infection models. Comparative genome analysis identified a novel 80K integrative conjugative element (ICE), ICESsuHN105, as required for the multidrug resistance phenotype. Six corresponding antibiotic resistance genes in this ICE were identified, namely tet (O), tet (M), erm (two copies), aph, and spc. Phylogenetic analysis classified the element as a homolog of the ICESa2603 family, containing the typical family backbone and insertion DNA. DNA hybrids mediated by natural transformation between HN105 and ZY05719 verified the antibiotic resistant genes of ICESsuHN105 that could be transferred successfully, while they were dispersedly inserted with a single gene in different genomic locations of ZY05719(HN105) transformants. To further identify the horizontal transfer of ICESsuHN105 as a whole mobile genetic element, a circular intermediate form of ICESsuHN105 was detected by PCR. However, the effective conjugation using serotype 2 S. suis as recipients was not observed in current assays in vitro. Further studies confirmed the presence of the complete lantibiotic locus encoded in ICESsuHN105 that effectively inhibits the growth of other streptococci. In summary, this study demonstrated the presence of antibiotic resistance genes in ICE that are able to transfer between different clinical isolates and adapt to a broader range of Streptococcus serotype or species.https://www.frontiersin.org/article/10.3389/fmicb.2019.00274/fullS. suis serotype 5comparative genomicsmulti-antibiotic resistanceintegrative and conjugative elementnatural transformationbacteriocin |
| spellingShingle | Yinchu Zhu Yinchu Zhu Yinchu Zhu Yue Zhang Yue Zhang Yue Zhang Jiale Ma Jiale Ma Jiale Ma Wenyang Dong Wenyang Dong Wenyang Dong Xiaojun Zhong Xiaojun Zhong Xiaojun Zhong Zihao Pan Zihao Pan Zihao Pan Huochun Yao Huochun Yao Huochun Yao ICESsuHN105, a Novel Multiple Antibiotic Resistant ICE in Streptococcus suis Serotype 5 Strain HN105 Frontiers in Microbiology S. suis serotype 5 comparative genomics multi-antibiotic resistance integrative and conjugative element natural transformation bacteriocin |
| title | ICESsuHN105, a Novel Multiple Antibiotic Resistant ICE in Streptococcus suis Serotype 5 Strain HN105 |
| title_full | ICESsuHN105, a Novel Multiple Antibiotic Resistant ICE in Streptococcus suis Serotype 5 Strain HN105 |
| title_fullStr | ICESsuHN105, a Novel Multiple Antibiotic Resistant ICE in Streptococcus suis Serotype 5 Strain HN105 |
| title_full_unstemmed | ICESsuHN105, a Novel Multiple Antibiotic Resistant ICE in Streptococcus suis Serotype 5 Strain HN105 |
| title_short | ICESsuHN105, a Novel Multiple Antibiotic Resistant ICE in Streptococcus suis Serotype 5 Strain HN105 |
| title_sort | icessuhn105 a novel multiple antibiotic resistant ice in streptococcus suis serotype 5 strain hn105 |
| topic | S. suis serotype 5 comparative genomics multi-antibiotic resistance integrative and conjugative element natural transformation bacteriocin |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00274/full |
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