Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform
The KRAS oncogene is involved in the pathogenesis of several types of cancer, particularly colorectal cancer (CRC). The most frequent mutations in this gene are associated with poor survival, increased tumor aggressiveness and resistance to therapy with anti-epidermal growth factor receptor (EGFR) a...
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MDPI AG
2018-01-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/18/1/131 |
| _version_ | 1817989728252723200 |
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| author | Maria Guarnaccia Rosario Iemmolo Floriana San Biagio Enrico Alessi Sebastiano Cavallaro |
| author_facet | Maria Guarnaccia Rosario Iemmolo Floriana San Biagio Enrico Alessi Sebastiano Cavallaro |
| author_sort | Maria Guarnaccia |
| collection | DOAJ |
| description | The KRAS oncogene is involved in the pathogenesis of several types of cancer, particularly colorectal cancer (CRC). The most frequent mutations in this gene are associated with poor survival, increased tumor aggressiveness and resistance to therapy with anti-epidermal growth factor receptor (EGFR) antibodies. For this reason, KRAS mutation testing has become increasingly common in clinical practice for personalized cancer treatments of CRC patients. Detection methods for KRAS mutations are currently expensive, laborious, time-consuming and often lack of diagnostic sensitivity and specificity. In this study, we describe the development of a Lab-on-Chip assay for genotyping of KRAS mutational status. This assay, based on the In-Check platform, integrates microfluidic handling, a multiplex polymerase chain reaction (PCR) and a low-density microarray. This integrated sample-to-result system enables the detection of KRAS point mutations, including those occurring in codons 12 and 13 of exon 2, 59 and 61 of exon 3, 117 and 146 of exon 4. Thanks to its miniaturization, automation, rapid analysis, minimal risk of sample contamination, increased accuracy and reproducibility of results, this Lab-on-Chip platform may offer immediate opportunities to simplify KRAS genotyping into clinical routine. |
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| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T00:50:33Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-3cb8fe78eec343df8da23a16e228e0922022-12-22T02:21:49ZengMDPI AGSensors1424-82202018-01-0118113110.3390/s18010131s18010131Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip PlatformMaria Guarnaccia0Rosario Iemmolo1Floriana San Biagio2Enrico Alessi3Sebastiano Cavallaro4Institute of Neurological Sciences, Italian National Research Council, Via Paolo Gaifami 18, 95126 Catania, ItalyInstitute of Neurological Sciences, Italian National Research Council, Via Paolo Gaifami 18, 95126 Catania, ItalySTMicroelectronics, Stradale Primosole 50, 95121 Catania, ItalyAnalog, MEMS & Sensor Group—HealthCare Business Development Unit, STMicroelectronics, Stradale Primosole 50, 95121 Catania, ItalyInstitute of Neurological Sciences, Italian National Research Council, Via Paolo Gaifami 18, 95126 Catania, ItalyThe KRAS oncogene is involved in the pathogenesis of several types of cancer, particularly colorectal cancer (CRC). The most frequent mutations in this gene are associated with poor survival, increased tumor aggressiveness and resistance to therapy with anti-epidermal growth factor receptor (EGFR) antibodies. For this reason, KRAS mutation testing has become increasingly common in clinical practice for personalized cancer treatments of CRC patients. Detection methods for KRAS mutations are currently expensive, laborious, time-consuming and often lack of diagnostic sensitivity and specificity. In this study, we describe the development of a Lab-on-Chip assay for genotyping of KRAS mutational status. This assay, based on the In-Check platform, integrates microfluidic handling, a multiplex polymerase chain reaction (PCR) and a low-density microarray. This integrated sample-to-result system enables the detection of KRAS point mutations, including those occurring in codons 12 and 13 of exon 2, 59 and 61 of exon 3, 117 and 146 of exon 4. Thanks to its miniaturization, automation, rapid analysis, minimal risk of sample contamination, increased accuracy and reproducibility of results, this Lab-on-Chip platform may offer immediate opportunities to simplify KRAS genotyping into clinical routine.http://www.mdpi.com/1424-8220/18/1/131Lab-on-ChipPCRMicroarraynucleic acids analysisKRASdiagnostic |
| spellingShingle | Maria Guarnaccia Rosario Iemmolo Floriana San Biagio Enrico Alessi Sebastiano Cavallaro Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform Sensors Lab-on-Chip PCR Microarray nucleic acids analysis KRAS diagnostic |
| title | Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform |
| title_full | Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform |
| title_fullStr | Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform |
| title_full_unstemmed | Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform |
| title_short | Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform |
| title_sort | genotyping of kras mutational status by the in check lab on chip platform |
| topic | Lab-on-Chip PCR Microarray nucleic acids analysis KRAS diagnostic |
| url | http://www.mdpi.com/1424-8220/18/1/131 |
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