Polyphenolic Compounds Extracted and Purified from <i>Buddleja Globosa</i> Hope (Buddlejaceae) Leaves Using Natural Deep Eutectic Solvents and Centrifugal Partition Chromatography
Chemical profiling of <i>Buddleja globosa</i> was performed by high-performance liquid chromatography coupled to electrospray ionization (HPLC-DAD-ESI-IT/MS) and quadrupole time-of-flight high-resolution mass spectrometry (HPLC-ESI-QTOF/MS). The identification of 17 main phenolic compoun...
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MDPI AG
2021-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/8/2192 |
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| author | Jeniffer Torres-Vega Sergio Gómez-Alonso José Pérez-Navarro Julio Alarcón-Enos Edgar Pastene-Navarrete |
| author_facet | Jeniffer Torres-Vega Sergio Gómez-Alonso José Pérez-Navarro Julio Alarcón-Enos Edgar Pastene-Navarrete |
| author_sort | Jeniffer Torres-Vega |
| collection | DOAJ |
| description | Chemical profiling of <i>Buddleja globosa</i> was performed by high-performance liquid chromatography coupled to electrospray ionization (HPLC-DAD-ESI-IT/MS) and quadrupole time-of-flight high-resolution mass spectrometry (HPLC-ESI-QTOF/MS). The identification of 17 main phenolic compounds in <i>B. globosa</i> leaf extracts was achieved. Along with caffeoyl glucoside isomers, caffeoylshikimic acid and several verbascoside derivatives (β-hydroxyverbascoside and β-hydroxyisoverbascoside) were identified. Among flavonoid compounds, the presence of 6-hydroxyluteolin-7-<i>O</i>-glucoside, quercetin-3-<i>O</i>-glucoside, luteolin 7-<i>O</i>-glucoside, apigenin 7-<i>O</i>-glucoside was confirmed. Campneoside I, forsythoside B, lipedoside A and forsythoside A were identified along with verbascoside, isoverbascoside, eukovoside and martynoside. The isolation of two bioactive phenolic compounds verbascoside and forsythoside B from <i>Buddleja globosa</i> (Buddlejaceae) was successfully achieved by centrifugal partition chromatography (CPC). Both compounds were obtained in one-step using optimized CPC methodology with the two-phase solvent system comprising ethyl acetate-n-butanol-ethanol-water (0.25:0.75:0.1:1, <i>v/v</i>). Additionally, eight Natural Deep Eutectic Solvents (NADESs) were tested for the extraction of polyphenols and compared with 80% methanol. The contents of verbascoside and luteolin 7-<i>O</i>-glucoside after extraction with 80% methanol were 26.165 and 3.206 mg/g, respectively. Among the NADESs tested in this study, proline- citric acid (1:1) and choline chloride-1, 2- propanediol (1:2) were the most promising solvents. With these NADES, extraction yields for verbascoside and luteolin 7-<i>O</i>-glucoside were 51.045 and 4.387 mg/g, respectively. Taken together, the results of this study confirm that CPC enabled the fast isolation of bioactive polyphenols from <i>B. globosa</i>. NADESs displayed higher extraction efficiency of phenolic and therefore could be used as an ecofriendly alternative to classic organic solvents. |
| first_indexed | 2024-03-10T12:25:56Z |
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| last_indexed | 2024-03-10T12:25:56Z |
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| spelling | doaj.art-3cbaa09274bb446f89617d827e63653b2023-11-21T15:02:43ZengMDPI AGMolecules1420-30492021-04-01268219210.3390/molecules26082192Polyphenolic Compounds Extracted and Purified from <i>Buddleja Globosa</i> Hope (Buddlejaceae) Leaves Using Natural Deep Eutectic Solvents and Centrifugal Partition ChromatographyJeniffer Torres-Vega0Sergio Gómez-Alonso1José Pérez-Navarro2Julio Alarcón-Enos3Edgar Pastene-Navarrete4Laboratorio de Farmacognosia, Departamento de Farmacia, Facultad de Farmacia, Universidad de Concepción, Concepción PC4030000, ChileRegional Institute for Applied Scientific Research, Faculty of Chemical Sciences, University of Castilla-La Mancha, PC13071 Castilla-La Mancha, SpainRegional Institute for Applied Scientific Research, Faculty of Chemical Sciences, University of Castilla-La Mancha, PC13071 Castilla-La Mancha, SpainLaboratorio de Síntesis y Biotransformación de Productos Naturales, Universidad del Bío-Bío, Chillán PC3800708, ChileLaboratorio de Farmacognosia, Departamento de Farmacia, Facultad de Farmacia, Universidad de Concepción, Concepción PC4030000, ChileChemical profiling of <i>Buddleja globosa</i> was performed by high-performance liquid chromatography coupled to electrospray ionization (HPLC-DAD-ESI-IT/MS) and quadrupole time-of-flight high-resolution mass spectrometry (HPLC-ESI-QTOF/MS). The identification of 17 main phenolic compounds in <i>B. globosa</i> leaf extracts was achieved. Along with caffeoyl glucoside isomers, caffeoylshikimic acid and several verbascoside derivatives (β-hydroxyverbascoside and β-hydroxyisoverbascoside) were identified. Among flavonoid compounds, the presence of 6-hydroxyluteolin-7-<i>O</i>-glucoside, quercetin-3-<i>O</i>-glucoside, luteolin 7-<i>O</i>-glucoside, apigenin 7-<i>O</i>-glucoside was confirmed. Campneoside I, forsythoside B, lipedoside A and forsythoside A were identified along with verbascoside, isoverbascoside, eukovoside and martynoside. The isolation of two bioactive phenolic compounds verbascoside and forsythoside B from <i>Buddleja globosa</i> (Buddlejaceae) was successfully achieved by centrifugal partition chromatography (CPC). Both compounds were obtained in one-step using optimized CPC methodology with the two-phase solvent system comprising ethyl acetate-n-butanol-ethanol-water (0.25:0.75:0.1:1, <i>v/v</i>). Additionally, eight Natural Deep Eutectic Solvents (NADESs) were tested for the extraction of polyphenols and compared with 80% methanol. The contents of verbascoside and luteolin 7-<i>O</i>-glucoside after extraction with 80% methanol were 26.165 and 3.206 mg/g, respectively. Among the NADESs tested in this study, proline- citric acid (1:1) and choline chloride-1, 2- propanediol (1:2) were the most promising solvents. With these NADES, extraction yields for verbascoside and luteolin 7-<i>O</i>-glucoside were 51.045 and 4.387 mg/g, respectively. Taken together, the results of this study confirm that CPC enabled the fast isolation of bioactive polyphenols from <i>B. globosa</i>. NADESs displayed higher extraction efficiency of phenolic and therefore could be used as an ecofriendly alternative to classic organic solvents.https://www.mdpi.com/1420-3049/26/8/2192centrifugal partition chromatographynatural deep eutectic solvents<i>Buddleja globosa</i>Maticophenylpropanoidsverbascoside |
| spellingShingle | Jeniffer Torres-Vega Sergio Gómez-Alonso José Pérez-Navarro Julio Alarcón-Enos Edgar Pastene-Navarrete Polyphenolic Compounds Extracted and Purified from <i>Buddleja Globosa</i> Hope (Buddlejaceae) Leaves Using Natural Deep Eutectic Solvents and Centrifugal Partition Chromatography Molecules centrifugal partition chromatography natural deep eutectic solvents <i>Buddleja globosa</i> Matico phenylpropanoids verbascoside |
| title | Polyphenolic Compounds Extracted and Purified from <i>Buddleja Globosa</i> Hope (Buddlejaceae) Leaves Using Natural Deep Eutectic Solvents and Centrifugal Partition Chromatography |
| title_full | Polyphenolic Compounds Extracted and Purified from <i>Buddleja Globosa</i> Hope (Buddlejaceae) Leaves Using Natural Deep Eutectic Solvents and Centrifugal Partition Chromatography |
| title_fullStr | Polyphenolic Compounds Extracted and Purified from <i>Buddleja Globosa</i> Hope (Buddlejaceae) Leaves Using Natural Deep Eutectic Solvents and Centrifugal Partition Chromatography |
| title_full_unstemmed | Polyphenolic Compounds Extracted and Purified from <i>Buddleja Globosa</i> Hope (Buddlejaceae) Leaves Using Natural Deep Eutectic Solvents and Centrifugal Partition Chromatography |
| title_short | Polyphenolic Compounds Extracted and Purified from <i>Buddleja Globosa</i> Hope (Buddlejaceae) Leaves Using Natural Deep Eutectic Solvents and Centrifugal Partition Chromatography |
| title_sort | polyphenolic compounds extracted and purified from i buddleja globosa i hope buddlejaceae leaves using natural deep eutectic solvents and centrifugal partition chromatography |
| topic | centrifugal partition chromatography natural deep eutectic solvents <i>Buddleja globosa</i> Matico phenylpropanoids verbascoside |
| url | https://www.mdpi.com/1420-3049/26/8/2192 |
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