Increased inflammation with crude E. coli LPS protects against acute leptospirosis in hamsters
ABSTRACTLeptospirosis is a worldwide zoonotic disease that causes acute kidney injury, liver disease, bleeding disorders, and even death. Treatment of the disease is largely dependent on the use of antibiotics, but recent studies on pathogenesis of leptospirosis have shown that immunomodulation may...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2019.1710435 |
| _version_ | 1797265859925770240 |
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| author | Wenlong Zhang Xufeng Xie Jiaqi Wang Ning Song Tianbao Lv Dianjun Wu Naisheng Zhang Yongguo Cao |
| author_facet | Wenlong Zhang Xufeng Xie Jiaqi Wang Ning Song Tianbao Lv Dianjun Wu Naisheng Zhang Yongguo Cao |
| author_sort | Wenlong Zhang |
| collection | DOAJ |
| description | ABSTRACTLeptospirosis is a worldwide zoonotic disease that causes acute kidney injury, liver disease, bleeding disorders, and even death. Treatment of the disease is largely dependent on the use of antibiotics, but recent studies on pathogenesis of leptospirosis have shown that immunomodulation may also be an effective treatment for this disease. Since the delay in inflammation correlates with higher pathogenicity of leptospira, we studied the effect of inducing inflammation on leptospirosis by using TLR4 activator LPS. In accordance with our hypothesis, treatment with LPS protected against leptospirosis by enhancing the inflammatory response in hamsters. The gene expression levels of TLR2, TLR4, NLRP3 and inflammatory factors were higher in LPS-treated group during leptospira infection in hamsters. Although the levels of NO and iNOS were higher in LPS-treated group than in Leptospira-infected group, the protective effect induced by LPS is iNOS-independent. Treatment with LPS induced higher anti-leptospira IgG level than infection with leptospira alone. Then, expressions of costimulatory molecules and maturation markers were analysed. The data showed that treatment with LPS enhanced the expression of CD40, CD80 and CD86. Our results indicate that increased inflammation induced by LPS derived from Escherichia coli (E. coli) protects against leptospirosis in hamsters. |
| first_indexed | 2024-03-07T17:23:50Z |
| format | Article |
| id | doaj.art-3cbe064b23d54786b5b81646db9db1f3 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-04-25T00:51:30Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-3cbe064b23d54786b5b81646db9db1f32024-03-11T16:04:24ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-019114014710.1080/22221751.2019.1710435Increased inflammation with crude E. coli LPS protects against acute leptospirosis in hamstersWenlong Zhang0Xufeng Xie1Jiaqi Wang2Ning Song3Tianbao Lv4Dianjun Wu5Naisheng Zhang6Yongguo Cao7Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, People’s Republic of ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, People’s Republic of ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, People’s Republic of ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, People’s Republic of ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, People’s Republic of ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, People’s Republic of ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, People’s Republic of ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, People’s Republic of ChinaABSTRACTLeptospirosis is a worldwide zoonotic disease that causes acute kidney injury, liver disease, bleeding disorders, and even death. Treatment of the disease is largely dependent on the use of antibiotics, but recent studies on pathogenesis of leptospirosis have shown that immunomodulation may also be an effective treatment for this disease. Since the delay in inflammation correlates with higher pathogenicity of leptospira, we studied the effect of inducing inflammation on leptospirosis by using TLR4 activator LPS. In accordance with our hypothesis, treatment with LPS protected against leptospirosis by enhancing the inflammatory response in hamsters. The gene expression levels of TLR2, TLR4, NLRP3 and inflammatory factors were higher in LPS-treated group during leptospira infection in hamsters. Although the levels of NO and iNOS were higher in LPS-treated group than in Leptospira-infected group, the protective effect induced by LPS is iNOS-independent. Treatment with LPS induced higher anti-leptospira IgG level than infection with leptospira alone. Then, expressions of costimulatory molecules and maturation markers were analysed. The data showed that treatment with LPS enhanced the expression of CD40, CD80 and CD86. Our results indicate that increased inflammation induced by LPS derived from Escherichia coli (E. coli) protects against leptospirosis in hamsters.https://www.tandfonline.com/doi/10.1080/22221751.2019.1710435LeptospirosisinflammationtreatmentLPShamster |
| spellingShingle | Wenlong Zhang Xufeng Xie Jiaqi Wang Ning Song Tianbao Lv Dianjun Wu Naisheng Zhang Yongguo Cao Increased inflammation with crude E. coli LPS protects against acute leptospirosis in hamsters Emerging Microbes and Infections Leptospirosis inflammation treatment LPS hamster |
| title | Increased inflammation with crude E. coli LPS protects against acute leptospirosis in hamsters |
| title_full | Increased inflammation with crude E. coli LPS protects against acute leptospirosis in hamsters |
| title_fullStr | Increased inflammation with crude E. coli LPS protects against acute leptospirosis in hamsters |
| title_full_unstemmed | Increased inflammation with crude E. coli LPS protects against acute leptospirosis in hamsters |
| title_short | Increased inflammation with crude E. coli LPS protects against acute leptospirosis in hamsters |
| title_sort | increased inflammation with crude e coli lps protects against acute leptospirosis in hamsters |
| topic | Leptospirosis inflammation treatment LPS hamster |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2019.1710435 |
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