Overexpressing rice lesion simulating disease 1-like gene (OsLOL1) in Gossypium hirsutum promotes somatic embryogenesis and plant regeneration
Abstract Background Cotton somatic embryogenesis is difficult or rarely frequent to present, which has limited gene function identification and biotechnological utility. Here, we employed a rice key somatic embryogenesis-related gene, rice lesion simulating disease 1-like gene (OsLOL1), to develop t...
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | Journal of Cotton Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s42397-020-00062-4 |
| _version_ | 1811223713559871488 |
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| author | Zhian WANG Peng WANG Guang HU Juanli XIAO Anhong ZHANG Xiaoli LUO Jiahe WU |
| author_facet | Zhian WANG Peng WANG Guang HU Juanli XIAO Anhong ZHANG Xiaoli LUO Jiahe WU |
| author_sort | Zhian WANG |
| collection | DOAJ |
| description | Abstract Background Cotton somatic embryogenesis is difficult or rarely frequent to present, which has limited gene function identification and biotechnological utility. Here, we employed a rice key somatic embryogenesis-related gene, rice lesion simulating disease 1-like gene (OsLOL1), to develop transgenic cotton callus for evaluating its function in ectopic plants. Results Overexpressing OsLOL1 can promote cotton callus to form embryogenic callus, not only shortening time but also increasing transition of somatic callus cells to embryogenic callus cells. And the regenerating plantlets per transgenic OsLOL1 embryogenic callus were significantly higher than those in the control transformed with empty vector. Analysis of physiological and biochemical showed that OsLOL1 can repress cotton superoxide dismutase 1 gene (GhSOD1) expression, possibly resulting in reactive oxidant species (ROS) accumulation in transgenic callus cells. And OsLOL1-overexpressed embryogenic callus exhibited higher α-amylase activity compared with the control, resulting from the promotion of OsLOL1 to cotton amylase 7 gene (GhAmy7) and GhAmy8 expression. Conclusion The data showed that OsLOL1 could be used as a candidate gene to transform cotton to increase its somatic embryogenesis capacity, facilitating gene function analysis and molecular breeding in cotton. |
| first_indexed | 2024-04-12T08:37:11Z |
| format | Article |
| id | doaj.art-3cc4b03e2c9746d6b0d9c211c5a422d7 |
| institution | Directory Open Access Journal |
| issn | 2523-3254 |
| language | English |
| last_indexed | 2024-04-12T08:37:11Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Cotton Research |
| spelling | doaj.art-3cc4b03e2c9746d6b0d9c211c5a422d72022-12-22T03:39:59ZengBMCJournal of Cotton Research2523-32542020-08-01311710.1186/s42397-020-00062-4Overexpressing rice lesion simulating disease 1-like gene (OsLOL1) in Gossypium hirsutum promotes somatic embryogenesis and plant regenerationZhian WANG0Peng WANG1Guang HU2Juanli XIAO3Anhong ZHANG4Xiaoli LUO5Jiahe WU6Institute of Cotton Research/Shanxi Academy of Agricultural Sciences/Shanxi Agricultural UniversityState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural SciencesState Key Laboratory of Plant Genomics/Institute of Microbiology, Chinese Academy of SciencesInstitute of Cotton Research/Shanxi Academy of Agricultural Sciences/Shanxi Agricultural UniversityInstitute of Cotton Research/Shanxi Academy of Agricultural Sciences/Shanxi Agricultural UniversityInstitute of Cotton Research/Shanxi Academy of Agricultural Sciences/Shanxi Agricultural UniversityState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural SciencesAbstract Background Cotton somatic embryogenesis is difficult or rarely frequent to present, which has limited gene function identification and biotechnological utility. Here, we employed a rice key somatic embryogenesis-related gene, rice lesion simulating disease 1-like gene (OsLOL1), to develop transgenic cotton callus for evaluating its function in ectopic plants. Results Overexpressing OsLOL1 can promote cotton callus to form embryogenic callus, not only shortening time but also increasing transition of somatic callus cells to embryogenic callus cells. And the regenerating plantlets per transgenic OsLOL1 embryogenic callus were significantly higher than those in the control transformed with empty vector. Analysis of physiological and biochemical showed that OsLOL1 can repress cotton superoxide dismutase 1 gene (GhSOD1) expression, possibly resulting in reactive oxidant species (ROS) accumulation in transgenic callus cells. And OsLOL1-overexpressed embryogenic callus exhibited higher α-amylase activity compared with the control, resulting from the promotion of OsLOL1 to cotton amylase 7 gene (GhAmy7) and GhAmy8 expression. Conclusion The data showed that OsLOL1 could be used as a candidate gene to transform cotton to increase its somatic embryogenesis capacity, facilitating gene function analysis and molecular breeding in cotton.http://link.springer.com/article/10.1186/s42397-020-00062-4Gossypium hirsutumSomatic embryogenesisOsLOL1GhSOD1GhAmy7 |
| spellingShingle | Zhian WANG Peng WANG Guang HU Juanli XIAO Anhong ZHANG Xiaoli LUO Jiahe WU Overexpressing rice lesion simulating disease 1-like gene (OsLOL1) in Gossypium hirsutum promotes somatic embryogenesis and plant regeneration Journal of Cotton Research Gossypium hirsutum Somatic embryogenesis OsLOL1 GhSOD1 GhAmy7 |
| title | Overexpressing rice lesion simulating disease 1-like gene (OsLOL1) in Gossypium hirsutum promotes somatic embryogenesis and plant regeneration |
| title_full | Overexpressing rice lesion simulating disease 1-like gene (OsLOL1) in Gossypium hirsutum promotes somatic embryogenesis and plant regeneration |
| title_fullStr | Overexpressing rice lesion simulating disease 1-like gene (OsLOL1) in Gossypium hirsutum promotes somatic embryogenesis and plant regeneration |
| title_full_unstemmed | Overexpressing rice lesion simulating disease 1-like gene (OsLOL1) in Gossypium hirsutum promotes somatic embryogenesis and plant regeneration |
| title_short | Overexpressing rice lesion simulating disease 1-like gene (OsLOL1) in Gossypium hirsutum promotes somatic embryogenesis and plant regeneration |
| title_sort | overexpressing rice lesion simulating disease 1 like gene oslol1 in gossypium hirsutum promotes somatic embryogenesis and plant regeneration |
| topic | Gossypium hirsutum Somatic embryogenesis OsLOL1 GhSOD1 GhAmy7 |
| url | http://link.springer.com/article/10.1186/s42397-020-00062-4 |
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