Biocidal Activity of a Nanoemulsion Containing Essential Oil from <i>Protium heptaphyllum</i> Resin against <i>Aedes aegypti</i> (Diptera: Culicidae)
This work aimed to prepare a nanoemulsion containing the essential oil of the <i>Protium heptaphyllum</i> resin and evaluate its biocidal activities against the different stages of development of the <i>Aedes aegypti</i> mosquito. Ovicide, pupicide, adulticide and repellency...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/21/6439 |
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| author | Cleidjane Gomes Faustino Fernando Antônio de Medeiros Allan Kardec Ribeiro Galardo Alex Bruno Lobato Rodrigues Anderson Luiz Pena da Costa Rosany Lopes Martins Lethicia Barreto Brandão Lizandra Lima Santos Marcos Antônio Alves de Medeiros Patrick de Castro Cantuária Ana Luzia Ferreira Farias Jader Santos Cruz Sheylla Susan Moreira da Silva de Almeida |
| author_facet | Cleidjane Gomes Faustino Fernando Antônio de Medeiros Allan Kardec Ribeiro Galardo Alex Bruno Lobato Rodrigues Anderson Luiz Pena da Costa Rosany Lopes Martins Lethicia Barreto Brandão Lizandra Lima Santos Marcos Antônio Alves de Medeiros Patrick de Castro Cantuária Ana Luzia Ferreira Farias Jader Santos Cruz Sheylla Susan Moreira da Silva de Almeida |
| author_sort | Cleidjane Gomes Faustino |
| collection | DOAJ |
| description | This work aimed to prepare a nanoemulsion containing the essential oil of the <i>Protium heptaphyllum</i> resin and evaluate its biocidal activities against the different stages of development of the <i>Aedes aegypti</i> mosquito. Ovicide, pupicide, adulticide and repellency assays were performed. The main constituents were <i>p</i>-cymene (27.70%) and <i>α</i>-pinene (22.31%). The developed nanoemulsion showed kinetic stability and monomodal distribution at a hydrophilic–lipophilic balance of 14 with a droplet size of 115.56 ± 1.68 nn and a zeta potential of −29.63 ± 3.46 mV. The nanoemulsion showed insecticidal action with LC<sub>50</sub> 0.404 µg·mL<sup>−1</sup> for the ovicidal effect. In the pupicidal test, at the concentration of 160 µg·mL<sup>−1</sup>, 100% mortality was reached after 24 h. For adulticidal activity, a diagnostic concentration of 200 µg·mL<sup>−1</sup> (120 min) was determined. In the repellency test, a concentration of 200 µg·mL<sup>−1</sup> during the 180 min of the test showed a protection index of 77.67%. In conclusion, the nanobiotechnological product derived from the essential oil of <i>P. heptaphyllum</i> resin can be considered as a promising colloid that can be used to control infectious disease vectors through a wide range of possible modes of applications, probably as this bioactive delivery system may allow the optimal effect of the <i>P. heptaphyllum</i> terpenes in aqueous media and may also induce satisfactory delivery to air interfaces. |
| first_indexed | 2024-03-10T05:55:41Z |
| format | Article |
| id | doaj.art-3cc6bebc6ba4431baabe3640dfc00041 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:55:41Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3cc6bebc6ba4431baabe3640dfc000412023-11-22T21:21:21ZengMDPI AGMolecules1420-30492021-10-012621643910.3390/molecules26216439Biocidal Activity of a Nanoemulsion Containing Essential Oil from <i>Protium heptaphyllum</i> Resin against <i>Aedes aegypti</i> (Diptera: Culicidae)Cleidjane Gomes Faustino0Fernando Antônio de Medeiros1Allan Kardec Ribeiro Galardo2Alex Bruno Lobato Rodrigues3Anderson Luiz Pena da Costa4Rosany Lopes Martins5Lethicia Barreto Brandão6Lizandra Lima Santos7Marcos Antônio Alves de Medeiros8Patrick de Castro Cantuária9Ana Luzia Ferreira Farias10Jader Santos Cruz11Sheylla Susan Moreira da Silva de Almeida12Postgraduate Program of Pharmaceutical Innovation, Laboratory of Pharmacognosy and Phytochemistry, Federal University of Amapá, Highway Juscelino Kubitschek, Macapá 68902-280, BrazilPostgraduate Program in Health Sciences, Federal University of Amapá, Highway Juscelino Kubitschek, Macapá 68902-280, BrazilInstitute of Scientific and Technological Research of the State of Amapá (IEPA), Highway Juscelino Kubitschek, Macapá 68903-419, BrazilPostgraduate Program in Biodiversity and Biotechnology Network BIONORTE, Laboratory of Pharmacognosy and Phytochemistry, Federal University of Amapá, Highway Juscelino Kubitschek, Macapá 68902-280, BrazilPostgraduate Program of Pharmaceutical Innovation, Laboratory of Pharmacognosy and Phytochemistry, Federal University of Amapá, Highway Juscelino Kubitschek, Macapá 68902-280, BrazilPostgraduate Program in Biodiversity and Biotechnology Network BIONORTE, Laboratory of Pharmacognosy and Phytochemistry, Federal University of Amapá, Highway Juscelino Kubitschek, Macapá 68902-280, BrazilPostgraduate Program of Pharmaceutical Innovation, Laboratory of Pharmacognosy and Phytochemistry, Federal University of Amapá, Highway Juscelino Kubitschek, Macapá 68902-280, BrazilPostgraduate Program of Pharmaceutical Innovation, Laboratory of Pharmacognosy and Phytochemistry, Federal University of Amapá, Highway Juscelino Kubitschek, Macapá 68902-280, BrazilFaculty of Medical Sciences of Paraíba BR 230, Km 9, Intermares-Cabedelo, Paraíba 58310-000, BrazilInstitute of Scientific and Technological Research of the State of Amapá (IEPA), Highway Juscelino Kubitschek, Macapá 68903-419, BrazilPostgraduate Program of Pharmaceutical Innovation, Laboratory of Pharmacognosy and Phytochemistry, Federal University of Amapá, Highway Juscelino Kubitschek, Macapá 68902-280, BrazilDepartment of Biochemistry and Immunology, Federal University of Minas Gerais, Avenida Antônio Carlos, Belo Horizonte 31270-901, BrazilPostgraduate Program of Pharmaceutical Innovation, Laboratory of Pharmacognosy and Phytochemistry, Federal University of Amapá, Highway Juscelino Kubitschek, Macapá 68902-280, BrazilThis work aimed to prepare a nanoemulsion containing the essential oil of the <i>Protium heptaphyllum</i> resin and evaluate its biocidal activities against the different stages of development of the <i>Aedes aegypti</i> mosquito. Ovicide, pupicide, adulticide and repellency assays were performed. The main constituents were <i>p</i>-cymene (27.70%) and <i>α</i>-pinene (22.31%). The developed nanoemulsion showed kinetic stability and monomodal distribution at a hydrophilic–lipophilic balance of 14 with a droplet size of 115.56 ± 1.68 nn and a zeta potential of −29.63 ± 3.46 mV. The nanoemulsion showed insecticidal action with LC<sub>50</sub> 0.404 µg·mL<sup>−1</sup> for the ovicidal effect. In the pupicidal test, at the concentration of 160 µg·mL<sup>−1</sup>, 100% mortality was reached after 24 h. For adulticidal activity, a diagnostic concentration of 200 µg·mL<sup>−1</sup> (120 min) was determined. In the repellency test, a concentration of 200 µg·mL<sup>−1</sup> during the 180 min of the test showed a protection index of 77.67%. In conclusion, the nanobiotechnological product derived from the essential oil of <i>P. heptaphyllum</i> resin can be considered as a promising colloid that can be used to control infectious disease vectors through a wide range of possible modes of applications, probably as this bioactive delivery system may allow the optimal effect of the <i>P. heptaphyllum</i> terpenes in aqueous media and may also induce satisfactory delivery to air interfaces.https://www.mdpi.com/1420-3049/26/21/6439<i>Protium heptaphyllum</i>essential oil<i>Aedes aegypti</i>biocidal activities |
| spellingShingle | Cleidjane Gomes Faustino Fernando Antônio de Medeiros Allan Kardec Ribeiro Galardo Alex Bruno Lobato Rodrigues Anderson Luiz Pena da Costa Rosany Lopes Martins Lethicia Barreto Brandão Lizandra Lima Santos Marcos Antônio Alves de Medeiros Patrick de Castro Cantuária Ana Luzia Ferreira Farias Jader Santos Cruz Sheylla Susan Moreira da Silva de Almeida Biocidal Activity of a Nanoemulsion Containing Essential Oil from <i>Protium heptaphyllum</i> Resin against <i>Aedes aegypti</i> (Diptera: Culicidae) Molecules <i>Protium heptaphyllum</i> essential oil <i>Aedes aegypti</i> biocidal activities |
| title | Biocidal Activity of a Nanoemulsion Containing Essential Oil from <i>Protium heptaphyllum</i> Resin against <i>Aedes aegypti</i> (Diptera: Culicidae) |
| title_full | Biocidal Activity of a Nanoemulsion Containing Essential Oil from <i>Protium heptaphyllum</i> Resin against <i>Aedes aegypti</i> (Diptera: Culicidae) |
| title_fullStr | Biocidal Activity of a Nanoemulsion Containing Essential Oil from <i>Protium heptaphyllum</i> Resin against <i>Aedes aegypti</i> (Diptera: Culicidae) |
| title_full_unstemmed | Biocidal Activity of a Nanoemulsion Containing Essential Oil from <i>Protium heptaphyllum</i> Resin against <i>Aedes aegypti</i> (Diptera: Culicidae) |
| title_short | Biocidal Activity of a Nanoemulsion Containing Essential Oil from <i>Protium heptaphyllum</i> Resin against <i>Aedes aegypti</i> (Diptera: Culicidae) |
| title_sort | biocidal activity of a nanoemulsion containing essential oil from i protium heptaphyllum i resin against i aedes aegypti i diptera culicidae |
| topic | <i>Protium heptaphyllum</i> essential oil <i>Aedes aegypti</i> biocidal activities |
| url | https://www.mdpi.com/1420-3049/26/21/6439 |
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