De novo formation of left-right asymmetry by posterior tilt of nodal cilia.
In the developing mouse embryo, leftward fluid flow on the ventral side of the node determines left-right (L-R) asymmetry. However, the mechanism by which the rotational movement of node cilia can generate a unidirectional flow remains hypothetical. Here we have addressed this question by motion and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2005-08-01
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| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.0030268 |
| _version_ | 1818742290944884736 |
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| author | Shigenori Nonaka Satoko Yoshiba Daisuke Watanabe Shingo Ikeuchi Tomonobu Goto Wallace F Marshall Hiroshi Hamada |
| author_facet | Shigenori Nonaka Satoko Yoshiba Daisuke Watanabe Shingo Ikeuchi Tomonobu Goto Wallace F Marshall Hiroshi Hamada |
| author_sort | Shigenori Nonaka |
| collection | DOAJ |
| description | In the developing mouse embryo, leftward fluid flow on the ventral side of the node determines left-right (L-R) asymmetry. However, the mechanism by which the rotational movement of node cilia can generate a unidirectional flow remains hypothetical. Here we have addressed this question by motion and morphological analyses of the node cilia and by fluid dynamic model experiments. We found that the cilia stand, not perpendicular to the node surface, but tilted posteriorly. We further confirmed that such posterior tilt can produce leftward flow in model experiments. These results strongly suggest that L-R asymmetry is not the descendant of pre-existing L-R asymmetry within each cell but is generated de novo by combining three sources of spatial information: antero-posterior and dorso-ventral axes, and the chirality of ciliary movement. |
| first_indexed | 2024-12-18T02:10:11Z |
| format | Article |
| id | doaj.art-3cc84f34efc340f69b1cdb7d2629fb68 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-18T02:10:11Z |
| publishDate | 2005-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-3cc84f34efc340f69b1cdb7d2629fb682022-12-21T21:24:30ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852005-08-0138e26810.1371/journal.pbio.0030268De novo formation of left-right asymmetry by posterior tilt of nodal cilia.Shigenori NonakaSatoko YoshibaDaisuke WatanabeShingo IkeuchiTomonobu GotoWallace F MarshallHiroshi HamadaIn the developing mouse embryo, leftward fluid flow on the ventral side of the node determines left-right (L-R) asymmetry. However, the mechanism by which the rotational movement of node cilia can generate a unidirectional flow remains hypothetical. Here we have addressed this question by motion and morphological analyses of the node cilia and by fluid dynamic model experiments. We found that the cilia stand, not perpendicular to the node surface, but tilted posteriorly. We further confirmed that such posterior tilt can produce leftward flow in model experiments. These results strongly suggest that L-R asymmetry is not the descendant of pre-existing L-R asymmetry within each cell but is generated de novo by combining three sources of spatial information: antero-posterior and dorso-ventral axes, and the chirality of ciliary movement.https://doi.org/10.1371/journal.pbio.0030268 |
| spellingShingle | Shigenori Nonaka Satoko Yoshiba Daisuke Watanabe Shingo Ikeuchi Tomonobu Goto Wallace F Marshall Hiroshi Hamada De novo formation of left-right asymmetry by posterior tilt of nodal cilia. PLoS Biology |
| title | De novo formation of left-right asymmetry by posterior tilt of nodal cilia. |
| title_full | De novo formation of left-right asymmetry by posterior tilt of nodal cilia. |
| title_fullStr | De novo formation of left-right asymmetry by posterior tilt of nodal cilia. |
| title_full_unstemmed | De novo formation of left-right asymmetry by posterior tilt of nodal cilia. |
| title_short | De novo formation of left-right asymmetry by posterior tilt of nodal cilia. |
| title_sort | de novo formation of left right asymmetry by posterior tilt of nodal cilia |
| url | https://doi.org/10.1371/journal.pbio.0030268 |
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