Brain-wide multi-fiber recording of neuronal activity in freely moving mice
Summary: While brain regions function in coordination to mediate diverse behaviors, techniques allowing simultaneous monitoring of many deep brain regions remain limited. Here, we present a multi-fiber recording protocol that enables simultaneous recording of fluorescence signals from multiple brain...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724000479 |
| _version_ | 1797317442928640000 |
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| author | Bing Dai Zhichao Guo Dayu Lin |
| author_facet | Bing Dai Zhichao Guo Dayu Lin |
| author_sort | Bing Dai |
| collection | DOAJ |
| description | Summary: While brain regions function in coordination to mediate diverse behaviors, techniques allowing simultaneous monitoring of many deep brain regions remain limited. Here, we present a multi-fiber recording protocol that enables simultaneous recording of fluorescence signals from multiple brain regions in freely behaving mice. We describe steps for assembling a multi-fiber array and patch cord, implantation, and recording. We then detail procedures for data extraction and visualization. This protocol enables a comprehensive view of the neural activity at the network level.For complete details on the use and execution of this protocol, please refer to Guo et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-08T03:35:00Z |
| format | Article |
| id | doaj.art-3cc9019d79a54a8b8bfbf2fb09de79c5 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-08T03:35:00Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-3cc9019d79a54a8b8bfbf2fb09de79c52024-02-10T04:45:31ZengElsevierSTAR Protocols2666-16672024-03-0151102882Brain-wide multi-fiber recording of neuronal activity in freely moving miceBing Dai0Zhichao Guo1Dayu Lin2Neuroscience Institute, New York University Langone Health, New York, NY 10016, USA; Corresponding authorNeuroscience Institute, New York University Langone Health, New York, NY 10016, USA; School of Life Sciences, Peking University, Beijing 100871, ChinaNeuroscience Institute, New York University Langone Health, New York, NY 10016, USA; Department of Psychiatry, New York University Langone Health, New York, NY, USA; Corresponding authorSummary: While brain regions function in coordination to mediate diverse behaviors, techniques allowing simultaneous monitoring of many deep brain regions remain limited. Here, we present a multi-fiber recording protocol that enables simultaneous recording of fluorescence signals from multiple brain regions in freely behaving mice. We describe steps for assembling a multi-fiber array and patch cord, implantation, and recording. We then detail procedures for data extraction and visualization. This protocol enables a comprehensive view of the neural activity at the network level.For complete details on the use and execution of this protocol, please refer to Guo et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724000479Model OrganismsNeuroscienceBehavior |
| spellingShingle | Bing Dai Zhichao Guo Dayu Lin Brain-wide multi-fiber recording of neuronal activity in freely moving mice STAR Protocols Model Organisms Neuroscience Behavior |
| title | Brain-wide multi-fiber recording of neuronal activity in freely moving mice |
| title_full | Brain-wide multi-fiber recording of neuronal activity in freely moving mice |
| title_fullStr | Brain-wide multi-fiber recording of neuronal activity in freely moving mice |
| title_full_unstemmed | Brain-wide multi-fiber recording of neuronal activity in freely moving mice |
| title_short | Brain-wide multi-fiber recording of neuronal activity in freely moving mice |
| title_sort | brain wide multi fiber recording of neuronal activity in freely moving mice |
| topic | Model Organisms Neuroscience Behavior |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724000479 |
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