Cellular Effects of Naringin in Prevention of Genotoxicity Caused by Mifepristone on Human Blood Lymphocytes
Background and purpose: Mifepristone is an oral synthetic steroid with antiglucocorticoid and antiprogesterone activities. Therefore, it could be dangerous during pregnancy since it causes cytogenetic damage and, consequently, abortion. Flavonoid compounds such as naringin have antioxidant propertie...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2018-06-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
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| Online Access: | http://jmums.mazums.ac.ir/article-1-10746-en.html |
| _version_ | 1797943769572573184 |
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| author | Mohammad Shokrzadeh Mahboube Rahmati Kukandeh Narges Kargar Darabi Mona Modanloo Marjan Fallah Abass Mohammadpour |
| author_facet | Mohammad Shokrzadeh Mahboube Rahmati Kukandeh Narges Kargar Darabi Mona Modanloo Marjan Fallah Abass Mohammadpour |
| author_sort | Mohammad Shokrzadeh |
| collection | DOAJ |
| description | Background and purpose: Mifepristone is an oral synthetic steroid with antiglucocorticoid and antiprogesterone activities. Therefore, it could be dangerous during pregnancy since it causes cytogenetic damage and, consequently, abortion. Flavonoid compounds such as naringin have antioxidant properties and eliminate various free radicals. Naringin is abundantly found in Iran, so, we investigated its cellular effects against cytogenetic damage caused by mifepristone on blood lymphocyte using micronucleus method.
Materials and Methods: In this experimental study, 5ml Venous blood were collected from 5 healthy and non-smokers using heparin syringe. Different naringin concentrations followed by 43µg/ml damage dose of mifepristone were injected to the cells. To evaluate the production of micronucleus in restrained binucleated lymphocytes in cytokine, the slides were prepared and evaluated by optical microscopy. The mean values were compared applying ANOVA test (posttest: Tukey) in SPSS and p<0.05 was regarded significant.
Results: The highest frequency of micronucleus was observed in the positive control group (17.67±1.528) and the lowest was seen in the naringin group at 232µg/ml (1.33+0.5774) (P<0.05). Naringin (232µg/ml) with mifepristone led to significant changes in the number of micronucleus nuclei compared to the sample without naringin.
Conclusion: Naringin is a potent antigenotoxin against the damage caused by mifepristonethan, therefore, it could be used as a protective agent against the toxic effects of mifepristone. |
| first_indexed | 2024-04-10T20:29:23Z |
| format | Article |
| id | doaj.art-3ccdfa61ab0a42499636cb961adc16a9 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:29:23Z |
| publishDate | 2018-06-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-3ccdfa61ab0a42499636cb961adc16a92023-01-25T07:33:19ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792018-06-0128161115120Cellular Effects of Naringin in Prevention of Genotoxicity Caused by Mifepristone on Human Blood LymphocytesMohammad Shokrzadeh0Mahboube Rahmati Kukandeh1Narges Kargar Darabi2Mona Modanloo3Marjan Fallah4Abass Mohammadpour5 ch Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran MSc Student in Genetics, Sana Institute of Higher Education, Sari, Iran MSc Student in Genetics, Sana Institute of Higher Education, Sari, Iran PhD in Pharmaceutical Sciences, Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran PhD Student in Toxicology, Pharmaceutical Sciences Research Center, Srudent Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran PhD Student in Cellular and Molecular Biology, Pharmaceutical Sciences Research Center, Srudent Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Background and purpose: Mifepristone is an oral synthetic steroid with antiglucocorticoid and antiprogesterone activities. Therefore, it could be dangerous during pregnancy since it causes cytogenetic damage and, consequently, abortion. Flavonoid compounds such as naringin have antioxidant properties and eliminate various free radicals. Naringin is abundantly found in Iran, so, we investigated its cellular effects against cytogenetic damage caused by mifepristone on blood lymphocyte using micronucleus method. Materials and Methods: In this experimental study, 5ml Venous blood were collected from 5 healthy and non-smokers using heparin syringe. Different naringin concentrations followed by 43µg/ml damage dose of mifepristone were injected to the cells. To evaluate the production of micronucleus in restrained binucleated lymphocytes in cytokine, the slides were prepared and evaluated by optical microscopy. The mean values were compared applying ANOVA test (posttest: Tukey) in SPSS and p<0.05 was regarded significant. Results: The highest frequency of micronucleus was observed in the positive control group (17.67±1.528) and the lowest was seen in the naringin group at 232µg/ml (1.33+0.5774) (P<0.05). Naringin (232µg/ml) with mifepristone led to significant changes in the number of micronucleus nuclei compared to the sample without naringin. Conclusion: Naringin is a potent antigenotoxin against the damage caused by mifepristonethan, therefore, it could be used as a protective agent against the toxic effects of mifepristone.http://jmums.mazums.ac.ir/article-1-10746-en.htmlmifepristonenaringingenotoxicitymicronucleus |
| spellingShingle | Mohammad Shokrzadeh Mahboube Rahmati Kukandeh Narges Kargar Darabi Mona Modanloo Marjan Fallah Abass Mohammadpour Cellular Effects of Naringin in Prevention of Genotoxicity Caused by Mifepristone on Human Blood Lymphocytes Journal of Mazandaran University of Medical Sciences mifepristone naringin genotoxicity micronucleus |
| title | Cellular Effects of Naringin in Prevention of Genotoxicity Caused by Mifepristone on Human Blood Lymphocytes |
| title_full | Cellular Effects of Naringin in Prevention of Genotoxicity Caused by Mifepristone on Human Blood Lymphocytes |
| title_fullStr | Cellular Effects of Naringin in Prevention of Genotoxicity Caused by Mifepristone on Human Blood Lymphocytes |
| title_full_unstemmed | Cellular Effects of Naringin in Prevention of Genotoxicity Caused by Mifepristone on Human Blood Lymphocytes |
| title_short | Cellular Effects of Naringin in Prevention of Genotoxicity Caused by Mifepristone on Human Blood Lymphocytes |
| title_sort | cellular effects of naringin in prevention of genotoxicity caused by mifepristone on human blood lymphocytes |
| topic | mifepristone naringin genotoxicity micronucleus |
| url | http://jmums.mazums.ac.ir/article-1-10746-en.html |
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