DNA barcoding reveals distinct population of Dopasia gracilis (Squamata: Anguidae) in Mizoram, Northeast India
The DNA barcode data of Asian Glass Lizard, Dopasia gracilis, is limited in the global database, especially from India. The present study aimed to generate a barcode sequence of morphologically identified D. gracilis from the Mizoram state in northeast India and compared with other Anguidae species....
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-07-01
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| Series: | Mitochondrial DNA. Part B. Resources |
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| Online Access: | http://dx.doi.org/10.1080/23802359.2020.1810147 |
| _version_ | 1797639062283091968 |
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| author | Shantanu Kundu Hmar Tlawmte Lalremsanga Lal Biakzuala Kailash Chandra Vikas Kumar |
| author_facet | Shantanu Kundu Hmar Tlawmte Lalremsanga Lal Biakzuala Kailash Chandra Vikas Kumar |
| author_sort | Shantanu Kundu |
| collection | DOAJ |
| description | The DNA barcode data of Asian Glass Lizard, Dopasia gracilis, is limited in the global database, especially from India. The present study aimed to generate a barcode sequence of morphologically identified D. gracilis from the Mizoram state in northeast India and compared with other Anguidae species. The studied species showed monophyletic clustering in the Bayesian analysis (BA) phylogeny with strong posterior probability support and also discriminated sufficient Kimura 2 parameter genetic distances. The barcode data of D. gracilis revealed high intra-species genetic variability and formed two clusters in BA phylogeny. The Templeton, Crandall, and Sing network also depicted four different haplotypes within the barcode sequences of D. gracilis. The DNA sequences generated from northeast India showed 6.5–6.6% and 7.3% genetic distances with the sequences generated from Yunnan Province and Tibetan Plateau, respectively. Considering the high genetic distances, multiple clustering, and distinct haplotypes, the present study assumed the presence of possible cryptic diversity of D. gracilis in the Indochina sub-region and a distinct population in northeast India. We recommended the generation of more DNA information from different localities to elucidate the actual diversity of D. gracilis within the known range distribution. |
| first_indexed | 2024-03-11T13:12:26Z |
| format | Article |
| id | doaj.art-3cce0bcdaf674910bc4714763b1c7785 |
| institution | Directory Open Access Journal |
| issn | 2380-2359 |
| language | English |
| last_indexed | 2024-03-11T13:12:26Z |
| publishDate | 2020-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj.art-3cce0bcdaf674910bc4714763b1c77852023-11-03T14:00:32ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592020-07-01533229323310.1080/23802359.2020.18101471810147DNA barcoding reveals distinct population of Dopasia gracilis (Squamata: Anguidae) in Mizoram, Northeast IndiaShantanu Kundu0Hmar Tlawmte Lalremsanga1Lal Biakzuala2Kailash Chandra3Vikas Kumar4Molecular Systematics Division, Zoological Survey of India, Centre for DNA TaxonomyDevelopmental Biology and Herpetology Laboratory, Department of Zoology, Mizoram UniversityDevelopmental Biology and Herpetology Laboratory, Department of Zoology, Mizoram UniversityMolecular Systematics Division, Zoological Survey of India, Centre for DNA TaxonomyMolecular Systematics Division, Zoological Survey of India, Centre for DNA TaxonomyThe DNA barcode data of Asian Glass Lizard, Dopasia gracilis, is limited in the global database, especially from India. The present study aimed to generate a barcode sequence of morphologically identified D. gracilis from the Mizoram state in northeast India and compared with other Anguidae species. The studied species showed monophyletic clustering in the Bayesian analysis (BA) phylogeny with strong posterior probability support and also discriminated sufficient Kimura 2 parameter genetic distances. The barcode data of D. gracilis revealed high intra-species genetic variability and formed two clusters in BA phylogeny. The Templeton, Crandall, and Sing network also depicted four different haplotypes within the barcode sequences of D. gracilis. The DNA sequences generated from northeast India showed 6.5–6.6% and 7.3% genetic distances with the sequences generated from Yunnan Province and Tibetan Plateau, respectively. Considering the high genetic distances, multiple clustering, and distinct haplotypes, the present study assumed the presence of possible cryptic diversity of D. gracilis in the Indochina sub-region and a distinct population in northeast India. We recommended the generation of more DNA information from different localities to elucidate the actual diversity of D. gracilis within the known range distribution.http://dx.doi.org/10.1080/23802359.2020.1810147indochinaglass lizardmitochondrial dnaphylogenyhaplotypes |
| spellingShingle | Shantanu Kundu Hmar Tlawmte Lalremsanga Lal Biakzuala Kailash Chandra Vikas Kumar DNA barcoding reveals distinct population of Dopasia gracilis (Squamata: Anguidae) in Mizoram, Northeast India Mitochondrial DNA. Part B. Resources indochina glass lizard mitochondrial dna phylogeny haplotypes |
| title | DNA barcoding reveals distinct population of Dopasia gracilis (Squamata: Anguidae) in Mizoram, Northeast India |
| title_full | DNA barcoding reveals distinct population of Dopasia gracilis (Squamata: Anguidae) in Mizoram, Northeast India |
| title_fullStr | DNA barcoding reveals distinct population of Dopasia gracilis (Squamata: Anguidae) in Mizoram, Northeast India |
| title_full_unstemmed | DNA barcoding reveals distinct population of Dopasia gracilis (Squamata: Anguidae) in Mizoram, Northeast India |
| title_short | DNA barcoding reveals distinct population of Dopasia gracilis (Squamata: Anguidae) in Mizoram, Northeast India |
| title_sort | dna barcoding reveals distinct population of dopasia gracilis squamata anguidae in mizoram northeast india |
| topic | indochina glass lizard mitochondrial dna phylogeny haplotypes |
| url | http://dx.doi.org/10.1080/23802359.2020.1810147 |
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