Characterization of <i>Leishmania</i> spp. Causing Cutaneous Lesions with a Negative Parasitological Diagnosis in Panama
A total of 123 DNA samples from Panamanian patients with cutaneous leishmaniasis (CL) lesions were evaluated. These samples were previously confirmed with CL by a specific KDNA-Viannia PCR but had a negative parasitological diagnosis (Group A). Epidemiological variables, such as age, sex, geographic...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2022-10-01
|
Series: | Tropical Medicine and Infectious Disease |
Subjects: | |
Online Access: | https://www.mdpi.com/2414-6366/7/10/282 |
_version_ | 1797469687088414720 |
---|---|
author | Adelys M. Reina Juan Castillo Mewa José E. Calzada Azael Saldaña |
author_facet | Adelys M. Reina Juan Castillo Mewa José E. Calzada Azael Saldaña |
author_sort | Adelys M. Reina |
collection | DOAJ |
description | A total of 123 DNA samples from Panamanian patients with cutaneous leishmaniasis (CL) lesions were evaluated. These samples were previously confirmed with CL by a specific KDNA-Viannia PCR but had a negative parasitological diagnosis (Group A). Epidemiological variables, such as age, sex, geographic origin, evolution time, and the number and location of the lesions, were analyzed. No significant differences (<i>p</i> < 0.05) were found when these variables were evaluated against a control panel of 123 CL lesion samples from CL patients with positive parasitological diagnoses (Group B). Of the 123 samples (Group A), 67% (82/123) gave positive results when re-analyzed by PCR-hsp70. An analysis of 69 of these samples via PCR-hsp70-RFLP showed that 59.4% (41/69) of the found restriction patterns corresponded to <i>Leishmania (Viannia) panamensis</i> and 40.6% (28/69) to <i>Leishmania (Viannia) guyanensis</i>. Finally, the sequence and phylogenetic analysis of 32 of the samples confirmed the species in 21 (65.6%, 21/32) samples, originally characterized as <i>L. (V.)</i> panamensis. However, 11 samples (34.4%, 11/32), initially identified via RFLP-Hsp70 as <i>L. (V.) guyanensis</i>, matched the sequence of a genetic variant known as <i>Leishmania</i> sp.1. These results point out the species/genetic variants of <i>Leishmania</i> in the case of CL lesions with an apparently low parasite load. |
first_indexed | 2024-03-09T19:24:44Z |
format | Article |
id | doaj.art-3ccead1020e34cb6904573c650859cf4 |
institution | Directory Open Access Journal |
issn | 2414-6366 |
language | English |
last_indexed | 2024-03-09T19:24:44Z |
publishDate | 2022-10-01 |
publisher | MDPI AG |
record_format | Article |
series | Tropical Medicine and Infectious Disease |
spelling | doaj.art-3ccead1020e34cb6904573c650859cf42023-11-24T03:00:19ZengMDPI AGTropical Medicine and Infectious Disease2414-63662022-10-0171028210.3390/tropicalmed7100282Characterization of <i>Leishmania</i> spp. Causing Cutaneous Lesions with a Negative Parasitological Diagnosis in PanamaAdelys M. Reina0Juan Castillo Mewa1José E. Calzada2Azael Saldaña3Departamento de Investigación en Parasitología, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panama 0816, PanamaDepartamento de Investigación en Genómica y Proteómica, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panama 0816, PanamaDepartamento de Investigación en Parasitología, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panama 0816, PanamaDepartamento de Investigación en Parasitología, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panama 0816, PanamaA total of 123 DNA samples from Panamanian patients with cutaneous leishmaniasis (CL) lesions were evaluated. These samples were previously confirmed with CL by a specific KDNA-Viannia PCR but had a negative parasitological diagnosis (Group A). Epidemiological variables, such as age, sex, geographic origin, evolution time, and the number and location of the lesions, were analyzed. No significant differences (<i>p</i> < 0.05) were found when these variables were evaluated against a control panel of 123 CL lesion samples from CL patients with positive parasitological diagnoses (Group B). Of the 123 samples (Group A), 67% (82/123) gave positive results when re-analyzed by PCR-hsp70. An analysis of 69 of these samples via PCR-hsp70-RFLP showed that 59.4% (41/69) of the found restriction patterns corresponded to <i>Leishmania (Viannia) panamensis</i> and 40.6% (28/69) to <i>Leishmania (Viannia) guyanensis</i>. Finally, the sequence and phylogenetic analysis of 32 of the samples confirmed the species in 21 (65.6%, 21/32) samples, originally characterized as <i>L. (V.)</i> panamensis. However, 11 samples (34.4%, 11/32), initially identified via RFLP-Hsp70 as <i>L. (V.) guyanensis</i>, matched the sequence of a genetic variant known as <i>Leishmania</i> sp.1. These results point out the species/genetic variants of <i>Leishmania</i> in the case of CL lesions with an apparently low parasite load.https://www.mdpi.com/2414-6366/7/10/282<i>Leishmania (Viannia) panamensis</i>diagnosiscutaneous leishmaniasisparasite loadmolecular characterizationPanamá |
spellingShingle | Adelys M. Reina Juan Castillo Mewa José E. Calzada Azael Saldaña Characterization of <i>Leishmania</i> spp. Causing Cutaneous Lesions with a Negative Parasitological Diagnosis in Panama Tropical Medicine and Infectious Disease <i>Leishmania (Viannia) panamensis</i> diagnosis cutaneous leishmaniasis parasite load molecular characterization Panamá |
title | Characterization of <i>Leishmania</i> spp. Causing Cutaneous Lesions with a Negative Parasitological Diagnosis in Panama |
title_full | Characterization of <i>Leishmania</i> spp. Causing Cutaneous Lesions with a Negative Parasitological Diagnosis in Panama |
title_fullStr | Characterization of <i>Leishmania</i> spp. Causing Cutaneous Lesions with a Negative Parasitological Diagnosis in Panama |
title_full_unstemmed | Characterization of <i>Leishmania</i> spp. Causing Cutaneous Lesions with a Negative Parasitological Diagnosis in Panama |
title_short | Characterization of <i>Leishmania</i> spp. Causing Cutaneous Lesions with a Negative Parasitological Diagnosis in Panama |
title_sort | characterization of i leishmania i spp causing cutaneous lesions with a negative parasitological diagnosis in panama |
topic | <i>Leishmania (Viannia) panamensis</i> diagnosis cutaneous leishmaniasis parasite load molecular characterization Panamá |
url | https://www.mdpi.com/2414-6366/7/10/282 |
work_keys_str_mv | AT adelysmreina characterizationofileishmaniaisppcausingcutaneouslesionswithanegativeparasitologicaldiagnosisinpanama AT juancastillomewa characterizationofileishmaniaisppcausingcutaneouslesionswithanegativeparasitologicaldiagnosisinpanama AT joseecalzada characterizationofileishmaniaisppcausingcutaneouslesionswithanegativeparasitologicaldiagnosisinpanama AT azaelsaldana characterizationofileishmaniaisppcausingcutaneouslesionswithanegativeparasitologicaldiagnosisinpanama |