Differential proteomic analysis of plasma-derived exosomes as diagnostic biomarkers for chronic HBV-related liver disease
Abstract Hepatitis B virus (HBV) infection is still a major public health problem worldwide. We aimed to identify new, non-invasive biomarkers for the early diagnosis of chronic HBV-related diseases, reveal alterations in the progression of chronic hepatitis B (CHB), liver cirrhosis (LC), and hepato...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-08-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13272-4 |
| _version_ | 1817998774036856832 |
|---|---|
| author | Bo Ye Yifei Shen Hui Chen Sha Lin Weilin Mao Yuejiao Dong Xuefen Li |
| author_facet | Bo Ye Yifei Shen Hui Chen Sha Lin Weilin Mao Yuejiao Dong Xuefen Li |
| author_sort | Bo Ye |
| collection | DOAJ |
| description | Abstract Hepatitis B virus (HBV) infection is still a major public health problem worldwide. We aimed to identify new, non-invasive biomarkers for the early diagnosis of chronic HBV-related diseases, reveal alterations in the progression of chronic hepatitis B (CHB), liver cirrhosis (LC), and hepatocellular carcinoma (HCC). Here, exosomes were isolated and characterized through size exclusion chromatography and nanoparticle tracking analysis. Profiles of differentially expressed proteins (DEPs) were analyzed through liquid chromatography-tandem mass spectrometry (LC–MS/MS), Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes analyses. Results showed that the DEPs, including CO9, LBP, SVEP1, and VWF levels in extracellular vesicles (EVs) were significantly higher in CHB than in healthy controls (HCs). VWF expression levels in EVs were significantly lower in CHB than in those with LC. KV311 expression levels in EVs were significantly higher, whereas LBP levels were significantly lower in patients with CHB than in those with HCC. All biomarkers seemed to exhibit a high diagnostic capacity for HBV-related liver disease. Patients with HBV-induced chronic liver disease exhibit characteristic protein profiles in their EVs. Thus, serum exosomes may be used as novel, liquid biopsy biomarkers to provide useful clinical information for the diagnosis of HBV-related liver diseases at different stages. |
| first_indexed | 2024-04-14T02:58:53Z |
| format | Article |
| id | doaj.art-3ccfaa9995324ff2a5ad16f6ca25679f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-14T02:58:53Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3ccfaa9995324ff2a5ad16f6ca25679f2022-12-22T02:15:59ZengNature PortfolioScientific Reports2045-23222022-08-0112111110.1038/s41598-022-13272-4Differential proteomic analysis of plasma-derived exosomes as diagnostic biomarkers for chronic HBV-related liver diseaseBo Ye0Yifei Shen1Hui Chen2Sha Lin3Weilin Mao4Yuejiao Dong5Xuefen Li6Department of Laboratory Medicine, Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, The First Affiliated Hospital, College of Medicine, Zhejiang University School of MedicineDepartment of Laboratory Medicine, Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, The First Affiliated Hospital, College of Medicine, Zhejiang University School of MedicineDepartment of Laboratory Medicine, Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, The First Affiliated Hospital, College of Medicine, Zhejiang University School of MedicineDepartment of Laboratory Medicine, Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, The First Affiliated Hospital, College of Medicine, Zhejiang University School of MedicineDepartment of Laboratory Medicine, Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, The First Affiliated Hospital, College of Medicine, Zhejiang University School of MedicineDepartment of Laboratory Medicine, Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, The First Affiliated Hospital, College of Medicine, Zhejiang University School of MedicineDepartment of Laboratory Medicine, Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, The First Affiliated Hospital, College of Medicine, Zhejiang University School of MedicineAbstract Hepatitis B virus (HBV) infection is still a major public health problem worldwide. We aimed to identify new, non-invasive biomarkers for the early diagnosis of chronic HBV-related diseases, reveal alterations in the progression of chronic hepatitis B (CHB), liver cirrhosis (LC), and hepatocellular carcinoma (HCC). Here, exosomes were isolated and characterized through size exclusion chromatography and nanoparticle tracking analysis. Profiles of differentially expressed proteins (DEPs) were analyzed through liquid chromatography-tandem mass spectrometry (LC–MS/MS), Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes analyses. Results showed that the DEPs, including CO9, LBP, SVEP1, and VWF levels in extracellular vesicles (EVs) were significantly higher in CHB than in healthy controls (HCs). VWF expression levels in EVs were significantly lower in CHB than in those with LC. KV311 expression levels in EVs were significantly higher, whereas LBP levels were significantly lower in patients with CHB than in those with HCC. All biomarkers seemed to exhibit a high diagnostic capacity for HBV-related liver disease. Patients with HBV-induced chronic liver disease exhibit characteristic protein profiles in their EVs. Thus, serum exosomes may be used as novel, liquid biopsy biomarkers to provide useful clinical information for the diagnosis of HBV-related liver diseases at different stages.https://doi.org/10.1038/s41598-022-13272-4 |
| spellingShingle | Bo Ye Yifei Shen Hui Chen Sha Lin Weilin Mao Yuejiao Dong Xuefen Li Differential proteomic analysis of plasma-derived exosomes as diagnostic biomarkers for chronic HBV-related liver disease Scientific Reports |
| title | Differential proteomic analysis of plasma-derived exosomes as diagnostic biomarkers for chronic HBV-related liver disease |
| title_full | Differential proteomic analysis of plasma-derived exosomes as diagnostic biomarkers for chronic HBV-related liver disease |
| title_fullStr | Differential proteomic analysis of plasma-derived exosomes as diagnostic biomarkers for chronic HBV-related liver disease |
| title_full_unstemmed | Differential proteomic analysis of plasma-derived exosomes as diagnostic biomarkers for chronic HBV-related liver disease |
| title_short | Differential proteomic analysis of plasma-derived exosomes as diagnostic biomarkers for chronic HBV-related liver disease |
| title_sort | differential proteomic analysis of plasma derived exosomes as diagnostic biomarkers for chronic hbv related liver disease |
| url | https://doi.org/10.1038/s41598-022-13272-4 |
| work_keys_str_mv | AT boye differentialproteomicanalysisofplasmaderivedexosomesasdiagnosticbiomarkersforchronichbvrelatedliverdisease AT yifeishen differentialproteomicanalysisofplasmaderivedexosomesasdiagnosticbiomarkersforchronichbvrelatedliverdisease AT huichen differentialproteomicanalysisofplasmaderivedexosomesasdiagnosticbiomarkersforchronichbvrelatedliverdisease AT shalin differentialproteomicanalysisofplasmaderivedexosomesasdiagnosticbiomarkersforchronichbvrelatedliverdisease AT weilinmao differentialproteomicanalysisofplasmaderivedexosomesasdiagnosticbiomarkersforchronichbvrelatedliverdisease AT yuejiaodong differentialproteomicanalysisofplasmaderivedexosomesasdiagnosticbiomarkersforchronichbvrelatedliverdisease AT xuefenli differentialproteomicanalysisofplasmaderivedexosomesasdiagnosticbiomarkersforchronichbvrelatedliverdisease |