Prevalence of metalo- β-lactamase-producing (MBL) Acinetobacter species in a tertiary care hospital
Background and Objectives: Acinetobacter causes a wide variety of illness in debilitated and hospitalized patients. Carbapenem resistance in Acinetobacter is an emerging problem and is a cause of concern as many nosocomial infections with Acinetobacter are resistant to most other antibiotics. The pr...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2014-02-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/470 |
| _version_ | 1818875551145787392 |
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| author | Amarjeet Kaur Veenu Gupta Deepinder Chhina |
| author_facet | Amarjeet Kaur Veenu Gupta Deepinder Chhina |
| author_sort | Amarjeet Kaur |
| collection | DOAJ |
| description | Background and Objectives: Acinetobacter causes a wide variety of illness in debilitated and hospitalized patients. Carbapenem resistance in Acinetobacter is an emerging problem and is a cause of concern as many nosocomial infections with Acinetobacter are resistant to most other antibiotics. The present study was aimed to study metallo-β-lactamase (MBL) production in Acinetobacter species.
Material and Methods: During one year prospective study, all isolates of Acinetobacter obtained from various clinical samples like respiratory, pus, blood and others were included. Antimicrobial susceptibility testing was done by standard Kirby Bauer disk diffusion method. Metallo β-lactamase (MBL) detection was done by imipenem-EDTA combined disk method.
Results: Among 1017 isolates, 964 were A. baumannii, 48 were A. lwoffii and 5 were A. hemolyticus. Out of these, majority of the isolates were obtained from respiratory samples, followed by pus. A. baumannii showed high level of resistance to cephalosporins, cotrimoxazole and piperacillin. A. lwoffii and A. hemolyticus showed lesser resistance to all antibiotics. Imipenem resistance was observed in 389 (40.3 %) isolates of A. baumannii and MBL activity was seen in 80.3% of isolates. MBL positive isolates of A. baumannii showed higher resistance as compared to MBL negative isolates.
Conclusion: This study demonstrated that multidrug resistant strains of Acinetobacter are common in tertiary care hospitals. Unwarranted and unrestricted usage of antibiotics is associated with emergence of resistance in nosocomial pathogens. Regular monitoring and documentation of carbapenem resistant is crucial in developing strategies to control infection due to these bacteria. |
| first_indexed | 2024-12-19T13:28:17Z |
| format | Article |
| id | doaj.art-3cd00965fe1f4bf0a1b147882e04c8eb |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-19T13:28:17Z |
| publishDate | 2014-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-3cd00965fe1f4bf0a1b147882e04c8eb2022-12-21T20:19:29ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472014-02-0161Prevalence of metalo- β-lactamase-producing (MBL) Acinetobacter species in a tertiary care hospitalAmarjeet Kaur0Veenu Gupta1Deepinder Chhina2Deparment of Lab Medicine, Medanta, The Medicity, New Delhi, India.Department of Microbiology, Dayanand Medical College & Hospital, Ludhiana, India.Department of Microbiology, Dayanand Medical College & Hospital, Ludhiana, India.Background and Objectives: Acinetobacter causes a wide variety of illness in debilitated and hospitalized patients. Carbapenem resistance in Acinetobacter is an emerging problem and is a cause of concern as many nosocomial infections with Acinetobacter are resistant to most other antibiotics. The present study was aimed to study metallo-β-lactamase (MBL) production in Acinetobacter species. Material and Methods: During one year prospective study, all isolates of Acinetobacter obtained from various clinical samples like respiratory, pus, blood and others were included. Antimicrobial susceptibility testing was done by standard Kirby Bauer disk diffusion method. Metallo β-lactamase (MBL) detection was done by imipenem-EDTA combined disk method. Results: Among 1017 isolates, 964 were A. baumannii, 48 were A. lwoffii and 5 were A. hemolyticus. Out of these, majority of the isolates were obtained from respiratory samples, followed by pus. A. baumannii showed high level of resistance to cephalosporins, cotrimoxazole and piperacillin. A. lwoffii and A. hemolyticus showed lesser resistance to all antibiotics. Imipenem resistance was observed in 389 (40.3 %) isolates of A. baumannii and MBL activity was seen in 80.3% of isolates. MBL positive isolates of A. baumannii showed higher resistance as compared to MBL negative isolates. Conclusion: This study demonstrated that multidrug resistant strains of Acinetobacter are common in tertiary care hospitals. Unwarranted and unrestricted usage of antibiotics is associated with emergence of resistance in nosocomial pathogens. Regular monitoring and documentation of carbapenem resistant is crucial in developing strategies to control infection due to these bacteria.https://ijm.tums.ac.ir/index.php/ijm/article/view/470A.baumanniiCarbapenem resistancemetallo ß-lactamase |
| spellingShingle | Amarjeet Kaur Veenu Gupta Deepinder Chhina Prevalence of metalo- β-lactamase-producing (MBL) Acinetobacter species in a tertiary care hospital Iranian Journal of Microbiology A.baumannii Carbapenem resistance metallo ß-lactamase |
| title | Prevalence of metalo- β-lactamase-producing (MBL) Acinetobacter species in a tertiary care hospital |
| title_full | Prevalence of metalo- β-lactamase-producing (MBL) Acinetobacter species in a tertiary care hospital |
| title_fullStr | Prevalence of metalo- β-lactamase-producing (MBL) Acinetobacter species in a tertiary care hospital |
| title_full_unstemmed | Prevalence of metalo- β-lactamase-producing (MBL) Acinetobacter species in a tertiary care hospital |
| title_short | Prevalence of metalo- β-lactamase-producing (MBL) Acinetobacter species in a tertiary care hospital |
| title_sort | prevalence of metalo β lactamase producing mbl acinetobacter species in a tertiary care hospital |
| topic | A.baumannii Carbapenem resistance metallo ß-lactamase |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/470 |
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