Preliminary evaluation of human hair follicle-derived dermal papilla cell characteristics
Dermal Papilla Cells (DPCs) are mesenchymal cells residing at the base of the hair follicles. They play an important role in the hair cycle remodeling and hair follicle regeneration. In this study, DPCs were isolated, and cultured from human hair follicles and evaluated the expression of -SMA, vers...
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| Format: | Article |
| Language: | English |
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HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE
2023-10-01
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| Series: | Ho Chi Minh City Open University Journal of Science - Engineering and Technology |
| Subjects: | |
| Online Access: | https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/2801 |
| _version_ | 1827576766946869248 |
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| author | Dao Thi Thanh Thuy Phan Van Tien Van Duc Huy Nguyen Thi Ngoc Nhu Phan Lu Chinh Nhan Nguyen Ton Ngoc Huynh Dang Thi Tung Loan |
| author_facet | Dao Thi Thanh Thuy Phan Van Tien Van Duc Huy Nguyen Thi Ngoc Nhu Phan Lu Chinh Nhan Nguyen Ton Ngoc Huynh Dang Thi Tung Loan |
| author_sort | Dao Thi Thanh Thuy |
| collection | DOAJ |
| description | Dermal Papilla Cells (DPCs) are mesenchymal cells residing at the base of the hair follicles. They play an important role in the hair cycle remodeling and hair follicle regeneration. In this study, DPCs were isolated, and cultured from human hair follicles and evaluated the expression of -SMA, versican by immunocytochemistry, and alkaline phosphatase activity. The human fibroblasts were used as a control to confirm the non-fibroblast DPCs. The results showed that the cells grew from the hair bulb and had fibroblast morphology. These bulb cells strongly expressed alkaline phosphatase activity -SMA, and versican while the human fibroblast lacked ALP activity and less expression of -SMA and versican. In conclusion, the protocol is established to isolate and culture DPCs from hair follicle bulbs, and ALP activity is suggested as the major marker for distinguishing DPCs and human fibroblasts. |
| first_indexed | 2024-03-08T21:16:08Z |
| format | Article |
| id | doaj.art-3cd6b917939e48dcbf4c1d09a4402605 |
| institution | Directory Open Access Journal |
| issn | 2734-9330 2734-9608 |
| language | English |
| last_indexed | 2024-03-08T21:16:08Z |
| publishDate | 2023-10-01 |
| publisher | HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE |
| record_format | Article |
| series | Ho Chi Minh City Open University Journal of Science - Engineering and Technology |
| spelling | doaj.art-3cd6b917939e48dcbf4c1d09a44026052023-12-21T15:42:30ZengHO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCEHo Chi Minh City Open University Journal of Science - Engineering and Technology2734-93302734-96082023-10-0113231110.46223/HCMCOUJS.tech.en.13.2.2801.20231968Preliminary evaluation of human hair follicle-derived dermal papilla cell characteristicsDao Thi Thanh Thuy0Phan Van Tien1Van Duc Huy2Nguyen Thi Ngoc Nhu3Phan Lu Chinh Nhan4Nguyen Ton Ngoc Huynh5Dang Thi Tung Loan6University of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh CityUniversity of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh CityUniversity of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh CityUniversity of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh CityUniversity of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh CityDNA International Hospital, Ho Chi Minh CityUniversity of Science, Ho Chi Minh City Vietnam National University, Ho Chi Minh CityDermal Papilla Cells (DPCs) are mesenchymal cells residing at the base of the hair follicles. They play an important role in the hair cycle remodeling and hair follicle regeneration. In this study, DPCs were isolated, and cultured from human hair follicles and evaluated the expression of -SMA, versican by immunocytochemistry, and alkaline phosphatase activity. The human fibroblasts were used as a control to confirm the non-fibroblast DPCs. The results showed that the cells grew from the hair bulb and had fibroblast morphology. These bulb cells strongly expressed alkaline phosphatase activity -SMA, and versican while the human fibroblast lacked ALP activity and less expression of -SMA and versican. In conclusion, the protocol is established to isolate and culture DPCs from hair follicle bulbs, and ALP activity is suggested as the major marker for distinguishing DPCs and human fibroblasts.https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/2801characteristicdermal papillafibroblasthair folliclehuman |
| spellingShingle | Dao Thi Thanh Thuy Phan Van Tien Van Duc Huy Nguyen Thi Ngoc Nhu Phan Lu Chinh Nhan Nguyen Ton Ngoc Huynh Dang Thi Tung Loan Preliminary evaluation of human hair follicle-derived dermal papilla cell characteristics Ho Chi Minh City Open University Journal of Science - Engineering and Technology characteristic dermal papilla fibroblast hair follicle human |
| title | Preliminary evaluation of human hair follicle-derived dermal papilla cell characteristics |
| title_full | Preliminary evaluation of human hair follicle-derived dermal papilla cell characteristics |
| title_fullStr | Preliminary evaluation of human hair follicle-derived dermal papilla cell characteristics |
| title_full_unstemmed | Preliminary evaluation of human hair follicle-derived dermal papilla cell characteristics |
| title_short | Preliminary evaluation of human hair follicle-derived dermal papilla cell characteristics |
| title_sort | preliminary evaluation of human hair follicle derived dermal papilla cell characteristics |
| topic | characteristic dermal papilla fibroblast hair follicle human |
| url | https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/2801 |
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