Evaluation of the Suitability of Mammalian <i>In Vitro</i> Assays to Assess the Genotoxic Potential of Food Contact Materials
Background: Non-targeted screening of food contact materials (FCM) for non-intentionally added substances (NIAS) reveals a great number of unknown and unidentified substances present at low concentrations. In the absence of toxicological data, the application of the threshold of toxicological concer...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/2/237 |
| _version_ | 1818554038121136128 |
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| author | Elisabeth Pinter Bernhard Rainer Thomas Czerny Elisabeth Riegel Benoît Schilter Maricel Marin-Kuan Manfred Tacker |
| author_facet | Elisabeth Pinter Bernhard Rainer Thomas Czerny Elisabeth Riegel Benoît Schilter Maricel Marin-Kuan Manfred Tacker |
| author_sort | Elisabeth Pinter |
| collection | DOAJ |
| description | Background: Non-targeted screening of food contact materials (FCM) for non-intentionally added substances (NIAS) reveals a great number of unknown and unidentified substances present at low concentrations. In the absence of toxicological data, the application of the threshold of toxicological concern (TTC) or of EU Regulation 10/2011 requires methods able to fulfill safety threshold criteria. In this review, mammalian <i>in vitro</i> genotoxicity assays are analyzed for their ability to detect DNA-damaging substances at limits of biological detection (LOBD) corresponding to the appropriate safety thresholds. Results: The ability of the assays to detect genotoxic effects varies greatly between substance classes. Especially for direct-acting mutagens, the assays lacked the ability to detect most DNA reactive substances below the threshold of 10 ppb, making them unsuitable to pick up potential genotoxicants present in FCM migrates. However, suitability for the detection of chromosomal damage or investigation of other modes of action makes them a complementary tool as part of a standard test battery aimed at giving additional information to ensure safety. Conclusion: improvements are necessary to comply with regulatory thresholds to consider mammalian genotoxicity <i>in vitro</i> assays to assess FCM safety. |
| first_indexed | 2024-12-12T09:33:50Z |
| format | Article |
| id | doaj.art-3cdeeb4b993a4a08b1c9cef333a1389b |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-12-12T09:33:50Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-3cdeeb4b993a4a08b1c9cef333a1389b2022-12-22T00:28:46ZengMDPI AGFoods2304-81582020-02-019223710.3390/foods9020237foods9020237Evaluation of the Suitability of Mammalian <i>In Vitro</i> Assays to Assess the Genotoxic Potential of Food Contact MaterialsElisabeth Pinter0Bernhard Rainer1Thomas Czerny2Elisabeth Riegel3Benoît Schilter4Maricel Marin-Kuan5Manfred Tacker6Department of Applied Life Sciences, University of Applied Sciences, FH Campus Wien, Helmut-Qualtinger-Gasse 2, 1030 Vienna, AustriaDepartment of Applied Life Sciences, University of Applied Sciences, FH Campus Wien, Helmut-Qualtinger-Gasse 2, 1030 Vienna, AustriaDepartment of Applied Life Sciences, University of Applied Sciences, FH Campus Wien, Helmut-Qualtinger-Gasse 2, 1030 Vienna, AustriaDepartment of Applied Life Sciences, University of Applied Sciences, FH Campus Wien, Helmut-Qualtinger-Gasse 2, 1030 Vienna, AustriaNestlé Research Center, Route du Jorat 57, 1000 Lausanne, SwitzerlandNestlé Research Center, Route du Jorat 57, 1000 Lausanne, SwitzerlandDepartment of Applied Life Sciences, University of Applied Sciences, FH Campus Wien, Helmut-Qualtinger-Gasse 2, 1030 Vienna, AustriaBackground: Non-targeted screening of food contact materials (FCM) for non-intentionally added substances (NIAS) reveals a great number of unknown and unidentified substances present at low concentrations. In the absence of toxicological data, the application of the threshold of toxicological concern (TTC) or of EU Regulation 10/2011 requires methods able to fulfill safety threshold criteria. In this review, mammalian <i>in vitro</i> genotoxicity assays are analyzed for their ability to detect DNA-damaging substances at limits of biological detection (LOBD) corresponding to the appropriate safety thresholds. Results: The ability of the assays to detect genotoxic effects varies greatly between substance classes. Especially for direct-acting mutagens, the assays lacked the ability to detect most DNA reactive substances below the threshold of 10 ppb, making them unsuitable to pick up potential genotoxicants present in FCM migrates. However, suitability for the detection of chromosomal damage or investigation of other modes of action makes them a complementary tool as part of a standard test battery aimed at giving additional information to ensure safety. Conclusion: improvements are necessary to comply with regulatory thresholds to consider mammalian genotoxicity <i>in vitro</i> assays to assess FCM safety.https://www.mdpi.com/2304-8158/9/2/237food contact materialgenotoxicityeukaryotic <i>in vitro</i> bioassayssafety assessmentnon-intentionally added substancespackaging |
| spellingShingle | Elisabeth Pinter Bernhard Rainer Thomas Czerny Elisabeth Riegel Benoît Schilter Maricel Marin-Kuan Manfred Tacker Evaluation of the Suitability of Mammalian <i>In Vitro</i> Assays to Assess the Genotoxic Potential of Food Contact Materials Foods food contact material genotoxicity eukaryotic <i>in vitro</i> bioassays safety assessment non-intentionally added substances packaging |
| title | Evaluation of the Suitability of Mammalian <i>In Vitro</i> Assays to Assess the Genotoxic Potential of Food Contact Materials |
| title_full | Evaluation of the Suitability of Mammalian <i>In Vitro</i> Assays to Assess the Genotoxic Potential of Food Contact Materials |
| title_fullStr | Evaluation of the Suitability of Mammalian <i>In Vitro</i> Assays to Assess the Genotoxic Potential of Food Contact Materials |
| title_full_unstemmed | Evaluation of the Suitability of Mammalian <i>In Vitro</i> Assays to Assess the Genotoxic Potential of Food Contact Materials |
| title_short | Evaluation of the Suitability of Mammalian <i>In Vitro</i> Assays to Assess the Genotoxic Potential of Food Contact Materials |
| title_sort | evaluation of the suitability of mammalian i in vitro i assays to assess the genotoxic potential of food contact materials |
| topic | food contact material genotoxicity eukaryotic <i>in vitro</i> bioassays safety assessment non-intentionally added substances packaging |
| url | https://www.mdpi.com/2304-8158/9/2/237 |
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