New Insights on the Impact of Cattle Handling on Post-Mortem Myofibrillar Muscle Proteome and Meat Tenderization
This study investigated the effect of different cattle management strategies at farm (Intensive vs. Extensive) and during transport and lairage (mixing vs. non-mixing with unfamiliar animals) on the myofibrillar subproteome of <i>Longissimus thoracis</i> et <i>lumborum</i> (L...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/10/12/3115 |
| _version_ | 1797504614110593024 |
|---|---|
| author | Verónica Sierra Laura González-Blanco Yolanda Diñeiro Fernando Díaz María Josefa García-Espina Ana Coto-Montes Mohammed Gagaoua Mamen Oliván |
| author_facet | Verónica Sierra Laura González-Blanco Yolanda Diñeiro Fernando Díaz María Josefa García-Espina Ana Coto-Montes Mohammed Gagaoua Mamen Oliván |
| author_sort | Verónica Sierra |
| collection | DOAJ |
| description | This study investigated the effect of different cattle management strategies at farm (Intensive vs. Extensive) and during transport and lairage (mixing vs. non-mixing with unfamiliar animals) on the myofibrillar subproteome of <i>Longissimus thoracis</i> et <i>lumborum</i> (LTL) muscle of “Asturiana de los Valles” yearling bulls. It further aimed to study the relationships with beef quality traits including pH, color, and tenderness evaluated by Warner–Bratzler shear force (WBSF). Thus, comparative proteomics of the myofibrillar fraction along meat maturation (from 2 h to 14 days <i>post-mortem</i>) and different quality traits were analyzed. A total of 23 protein fragments corresponding to 21 unique proteins showed significant differences among the treatments (<i>p</i> < 0.05) due to any of the factors considered (Farm, Transport and Lairage, and <i>post-mortem</i> time ageing). The proteins belong to several biological pathways including three structural proteins (MYBPC2, TNNT3, and MYL1) and one metabolic enzyme (ALDOA) that were affected by both Farm and Transport/Lairage factors. ACTA1, LDB3, and FHL2 were affected by Farm factors, while TNNI2 and MYLPF (structural proteins), PKM (metabolic enzyme), and HSPB1 (small Heat shock protein) were affected by Transport/Lairage factors. Several correlations were found between the changing proteins (PKM, ALDOA, TNNI2, TNNT3, ACTA1, MYL1, and CRYAB) and color and tenderness beef quality traits, indicating their importance in the determination of meat quality and their possible use as putative biomarkers. |
| first_indexed | 2024-03-10T04:07:04Z |
| format | Article |
| id | doaj.art-3cdf3a8b643d4a3ca05b78392cbda4f8 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T04:07:04Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-3cdf3a8b643d4a3ca05b78392cbda4f82023-11-23T08:19:02ZengMDPI AGFoods2304-81582021-12-011012311510.3390/foods10123115New Insights on the Impact of Cattle Handling on Post-Mortem Myofibrillar Muscle Proteome and Meat TenderizationVerónica Sierra0Laura González-Blanco1Yolanda Diñeiro2Fernando Díaz3María Josefa García-Espina4Ana Coto-Montes5Mohammed Gagaoua6Mamen Oliván7Área de Sistemas de Producción Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Ctra. AS-267, PK 19, 33300 Villaviciosa, SpainÁrea de Sistemas de Producción Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Ctra. AS-267, PK 19, 33300 Villaviciosa, SpainÁrea de Sistemas de Producción Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Ctra. AS-267, PK 19, 33300 Villaviciosa, SpainÁrea de Sistemas de Producción Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Ctra. AS-267, PK 19, 33300 Villaviciosa, SpainÁrea de Sistemas de Producción Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Ctra. AS-267, PK 19, 33300 Villaviciosa, SpainInstituto de Investigación Sanitaria del Principado de Asturias (ISPA), Av. del Hospital Universitario, s/n, 33011 Oviedo, SpainFood Quality and Sensory Science Department, Teagasc Food Research Centre, Dublin 15, D15 KN3K Ashtown, IrelandÁrea de Sistemas de Producción Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Ctra. AS-267, PK 19, 33300 Villaviciosa, SpainThis study investigated the effect of different cattle management strategies at farm (Intensive vs. Extensive) and during transport and lairage (mixing vs. non-mixing with unfamiliar animals) on the myofibrillar subproteome of <i>Longissimus thoracis</i> et <i>lumborum</i> (LTL) muscle of “Asturiana de los Valles” yearling bulls. It further aimed to study the relationships with beef quality traits including pH, color, and tenderness evaluated by Warner–Bratzler shear force (WBSF). Thus, comparative proteomics of the myofibrillar fraction along meat maturation (from 2 h to 14 days <i>post-mortem</i>) and different quality traits were analyzed. A total of 23 protein fragments corresponding to 21 unique proteins showed significant differences among the treatments (<i>p</i> < 0.05) due to any of the factors considered (Farm, Transport and Lairage, and <i>post-mortem</i> time ageing). The proteins belong to several biological pathways including three structural proteins (MYBPC2, TNNT3, and MYL1) and one metabolic enzyme (ALDOA) that were affected by both Farm and Transport/Lairage factors. ACTA1, LDB3, and FHL2 were affected by Farm factors, while TNNI2 and MYLPF (structural proteins), PKM (metabolic enzyme), and HSPB1 (small Heat shock protein) were affected by Transport/Lairage factors. Several correlations were found between the changing proteins (PKM, ALDOA, TNNI2, TNNT3, ACTA1, MYL1, and CRYAB) and color and tenderness beef quality traits, indicating their importance in the determination of meat quality and their possible use as putative biomarkers.https://www.mdpi.com/2304-8158/10/12/3115intensive managementextensive managementmixing unfamiliar animalsmyofibrillar proteinspre-slaughter stressprotein biomarkers |
| spellingShingle | Verónica Sierra Laura González-Blanco Yolanda Diñeiro Fernando Díaz María Josefa García-Espina Ana Coto-Montes Mohammed Gagaoua Mamen Oliván New Insights on the Impact of Cattle Handling on Post-Mortem Myofibrillar Muscle Proteome and Meat Tenderization Foods intensive management extensive management mixing unfamiliar animals myofibrillar proteins pre-slaughter stress protein biomarkers |
| title | New Insights on the Impact of Cattle Handling on Post-Mortem Myofibrillar Muscle Proteome and Meat Tenderization |
| title_full | New Insights on the Impact of Cattle Handling on Post-Mortem Myofibrillar Muscle Proteome and Meat Tenderization |
| title_fullStr | New Insights on the Impact of Cattle Handling on Post-Mortem Myofibrillar Muscle Proteome and Meat Tenderization |
| title_full_unstemmed | New Insights on the Impact of Cattle Handling on Post-Mortem Myofibrillar Muscle Proteome and Meat Tenderization |
| title_short | New Insights on the Impact of Cattle Handling on Post-Mortem Myofibrillar Muscle Proteome and Meat Tenderization |
| title_sort | new insights on the impact of cattle handling on post mortem myofibrillar muscle proteome and meat tenderization |
| topic | intensive management extensive management mixing unfamiliar animals myofibrillar proteins pre-slaughter stress protein biomarkers |
| url | https://www.mdpi.com/2304-8158/10/12/3115 |
| work_keys_str_mv | AT veronicasierra newinsightsontheimpactofcattlehandlingonpostmortemmyofibrillarmuscleproteomeandmeattenderization AT lauragonzalezblanco newinsightsontheimpactofcattlehandlingonpostmortemmyofibrillarmuscleproteomeandmeattenderization AT yolandadineiro newinsightsontheimpactofcattlehandlingonpostmortemmyofibrillarmuscleproteomeandmeattenderization AT fernandodiaz newinsightsontheimpactofcattlehandlingonpostmortemmyofibrillarmuscleproteomeandmeattenderization AT mariajosefagarciaespina newinsightsontheimpactofcattlehandlingonpostmortemmyofibrillarmuscleproteomeandmeattenderization AT anacotomontes newinsightsontheimpactofcattlehandlingonpostmortemmyofibrillarmuscleproteomeandmeattenderization AT mohammedgagaoua newinsightsontheimpactofcattlehandlingonpostmortemmyofibrillarmuscleproteomeandmeattenderization AT mamenolivan newinsightsontheimpactofcattlehandlingonpostmortemmyofibrillarmuscleproteomeandmeattenderization |