In Vitro Fumonisin Biosynthesis and Genetic Structure of <i>Fusarium verticillioides</i> Strains from Five Mediterranean Countries
Investigating the in vitro fumonisin biosynthesis and the genetic structure of <i>Fusarium verticillioides</i> populations can provide important insights into the relationships between strains originating from various world regions. In this study, 90 <i>F. verticillioides</i>...
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MDPI AG
2020-02-01
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| author | Giovanni Beccari Łukasz Stępień Andrea Onofri Veronica M. T. Lattanzio Biancamaria Ciasca Sally I. Abd-El Fatah Francesco Valente Monika Urbaniak Lorenzo Covarelli |
| author_facet | Giovanni Beccari Łukasz Stępień Andrea Onofri Veronica M. T. Lattanzio Biancamaria Ciasca Sally I. Abd-El Fatah Francesco Valente Monika Urbaniak Lorenzo Covarelli |
| author_sort | Giovanni Beccari |
| collection | DOAJ |
| description | Investigating the in vitro fumonisin biosynthesis and the genetic structure of <i>Fusarium verticillioides</i> populations can provide important insights into the relationships between strains originating from various world regions. In this study, 90 <i>F. verticillioides</i> strains isolated from maize in five Mediterranean countries (Italy, Spain, Tunisia, Egypt and Iran) were analyzed to investigate their ability to in vitro biosynthesize fumonisin B<sub>1</sub>, fumonisin B<sub>2</sub> and fumonisin B<sub>3</sub> and to characterize their genetic profile. In general, 80% of the analyzed strains were able to biosynthesize fumonisins (range 0.03−69.84 μg/g). Populations from Italy, Spain, Tunisia and Iran showed a similar percentage of fumonisin producing strains (>90%); conversely, the Egyptian population showed a lower level of producing strains (46%). Significant differences in fumonisin biosynthesis were detected among strains isolated in the same country and among strains isolated from different countries. A portion of the divergent <i>FUM1</i> gene and of intergenic regions <i>FUM6</i>-<i>FUM7</i> and <i>FUM7</i>-<i>FUM8</i> were sequenced to evaluate strain diversity among populations. A high level of genetic uniformity inside the populations analyzed was detected. Apparently, neither geographical origin nor fumonisin production ability were correlated to the genetic diversity of the strain set. However, four strains from Egypt differed from the remaining strains. |
| first_indexed | 2024-12-11T09:24:58Z |
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| last_indexed | 2024-12-11T09:24:58Z |
| publishDate | 2020-02-01 |
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| series | Microorganisms |
| spelling | doaj.art-3cdf787f87094d27890904e82222611f2022-12-22T01:13:10ZengMDPI AGMicroorganisms2076-26072020-02-018224110.3390/microorganisms8020241microorganisms8020241In Vitro Fumonisin Biosynthesis and Genetic Structure of <i>Fusarium verticillioides</i> Strains from Five Mediterranean CountriesGiovanni Beccari0Łukasz Stępień1Andrea Onofri2Veronica M. T. Lattanzio3Biancamaria Ciasca4Sally I. Abd-El Fatah5Francesco Valente6Monika Urbaniak7Lorenzo Covarelli8Department of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, ItalyDepartment of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, ItalyNational Research Council of Italy, Institute of Sciences of Food Production (ISPA-CNR), 70126 Bari, ItalyNational Research Council of Italy, Institute of Sciences of Food Production (ISPA-CNR), 70126 Bari, ItalyFood Toxins and Contaminants Department, National Research Centre, Cairo 12622, EgyptDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, ItalyDepartment of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, PolandDepartment of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, ItalyInvestigating the in vitro fumonisin biosynthesis and the genetic structure of <i>Fusarium verticillioides</i> populations can provide important insights into the relationships between strains originating from various world regions. In this study, 90 <i>F. verticillioides</i> strains isolated from maize in five Mediterranean countries (Italy, Spain, Tunisia, Egypt and Iran) were analyzed to investigate their ability to in vitro biosynthesize fumonisin B<sub>1</sub>, fumonisin B<sub>2</sub> and fumonisin B<sub>3</sub> and to characterize their genetic profile. In general, 80% of the analyzed strains were able to biosynthesize fumonisins (range 0.03−69.84 μg/g). Populations from Italy, Spain, Tunisia and Iran showed a similar percentage of fumonisin producing strains (>90%); conversely, the Egyptian population showed a lower level of producing strains (46%). Significant differences in fumonisin biosynthesis were detected among strains isolated in the same country and among strains isolated from different countries. A portion of the divergent <i>FUM1</i> gene and of intergenic regions <i>FUM6</i>-<i>FUM7</i> and <i>FUM7</i>-<i>FUM8</i> were sequenced to evaluate strain diversity among populations. A high level of genetic uniformity inside the populations analyzed was detected. Apparently, neither geographical origin nor fumonisin production ability were correlated to the genetic diversity of the strain set. However, four strains from Egypt differed from the remaining strains.https://www.mdpi.com/2076-2607/8/2/241<i>fusarium</i>ear rotmaizefumonisins<i>fum1</i> |
| spellingShingle | Giovanni Beccari Łukasz Stępień Andrea Onofri Veronica M. T. Lattanzio Biancamaria Ciasca Sally I. Abd-El Fatah Francesco Valente Monika Urbaniak Lorenzo Covarelli In Vitro Fumonisin Biosynthesis and Genetic Structure of <i>Fusarium verticillioides</i> Strains from Five Mediterranean Countries Microorganisms <i>fusarium</i> ear rot maize fumonisins <i>fum1</i> |
| title | In Vitro Fumonisin Biosynthesis and Genetic Structure of <i>Fusarium verticillioides</i> Strains from Five Mediterranean Countries |
| title_full | In Vitro Fumonisin Biosynthesis and Genetic Structure of <i>Fusarium verticillioides</i> Strains from Five Mediterranean Countries |
| title_fullStr | In Vitro Fumonisin Biosynthesis and Genetic Structure of <i>Fusarium verticillioides</i> Strains from Five Mediterranean Countries |
| title_full_unstemmed | In Vitro Fumonisin Biosynthesis and Genetic Structure of <i>Fusarium verticillioides</i> Strains from Five Mediterranean Countries |
| title_short | In Vitro Fumonisin Biosynthesis and Genetic Structure of <i>Fusarium verticillioides</i> Strains from Five Mediterranean Countries |
| title_sort | in vitro fumonisin biosynthesis and genetic structure of i fusarium verticillioides i strains from five mediterranean countries |
| topic | <i>fusarium</i> ear rot maize fumonisins <i>fum1</i> |
| url | https://www.mdpi.com/2076-2607/8/2/241 |
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