Assessment of Methylobacterium oryzae CBMB20 aggregates for salt tolerance and plant growth promoting characteristics for bio-inoculant development
Abstract Salinity is one of the major factors contributing to the loss of crop productivity and thereby impacting livelihood of people in more than 100 countries of the world and the area of land affected by salinity is increasing day by day. This will worsen due to various factors such as drought t...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-11-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-017-0518-7 |
| _version_ | 1818963573206941696 |
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| author | Mak Chanratana Gwang Hyun Han Aritra Roy Choudhury Seshadri Sundaram Md. Abdul Halim Ramasamy Krishnamoorthy Yeongyeong Kang Tongmin Sa |
| author_facet | Mak Chanratana Gwang Hyun Han Aritra Roy Choudhury Seshadri Sundaram Md. Abdul Halim Ramasamy Krishnamoorthy Yeongyeong Kang Tongmin Sa |
| author_sort | Mak Chanratana |
| collection | DOAJ |
| description | Abstract Salinity is one of the major factors contributing to the loss of crop productivity and thereby impacting livelihood of people in more than 100 countries of the world and the area of land affected by salinity is increasing day by day. This will worsen due to various factors such as drought that might result in high soil salinity. Use of plant growth promoting rhizobacteria is one of the promising eco-friendly strategies for salinity stress management as part of sustainable agricultural practices. However, it requires selecting rhizobacteria with good survivability and adaptation to salt stress. In this study we report aggregation of Methylobacterium oryzae CBMB20 cells grown in media containing high C/N ratio (30:1) than in media containing low C/N ratio (7:1). Aggregated Methylobacterium oryzae CBMB20 cells exhibited enhanced tolerance to UV irradiation, heat, desiccation, different temperature regimes, oxidative stress, starvation and supported higher population in media. Poly-β-hydroxybutyrate accumulation, exopolysaccharide production, proline accumulation and biofilm formation were good at 100 mM salt concentration with good microbial cell hydrophobicity at both 50 and 100 mM than other concentrations. Both the aggregated and non-aggregated cells grown under 0–200 mM salt concentrations produced IAA even at 200 mM salt concentration with a peak at 100 mM concentration with aggregated cells producing significantly higher quantities. ACC deaminase activity was observed in all NaCl concentrations studied with gradual and drastic reduction in aggregated and non-aggregated cells over increased salt concentrations. |
| first_indexed | 2024-12-20T12:47:22Z |
| format | Article |
| id | doaj.art-3cdf9d1db8f04372844070605d2bb585 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-20T12:47:22Z |
| publishDate | 2017-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-3cdf9d1db8f04372844070605d2bb5852022-12-21T19:40:16ZengSpringerOpenAMB Express2191-08552017-11-017111010.1186/s13568-017-0518-7Assessment of Methylobacterium oryzae CBMB20 aggregates for salt tolerance and plant growth promoting characteristics for bio-inoculant developmentMak Chanratana0Gwang Hyun Han1Aritra Roy Choudhury2Seshadri Sundaram3Md. Abdul Halim4Ramasamy Krishnamoorthy5Yeongyeong Kang6Tongmin Sa7Department of Environmental and Biological Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National UniversityDepartment of Environmental and Biological Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National UniversityDepartment of Environmental and Biological Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National UniversityDepartment of Environmental and Biological Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National UniversityDepartment of Environmental and Biological Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National UniversityTamil Nadu Agricultural UniversityDepartment of Environmental and Biological Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National UniversityDepartment of Environmental and Biological Chemistry, College of Agriculture, Life and Environment Sciences, Chungbuk National UniversityAbstract Salinity is one of the major factors contributing to the loss of crop productivity and thereby impacting livelihood of people in more than 100 countries of the world and the area of land affected by salinity is increasing day by day. This will worsen due to various factors such as drought that might result in high soil salinity. Use of plant growth promoting rhizobacteria is one of the promising eco-friendly strategies for salinity stress management as part of sustainable agricultural practices. However, it requires selecting rhizobacteria with good survivability and adaptation to salt stress. In this study we report aggregation of Methylobacterium oryzae CBMB20 cells grown in media containing high C/N ratio (30:1) than in media containing low C/N ratio (7:1). Aggregated Methylobacterium oryzae CBMB20 cells exhibited enhanced tolerance to UV irradiation, heat, desiccation, different temperature regimes, oxidative stress, starvation and supported higher population in media. Poly-β-hydroxybutyrate accumulation, exopolysaccharide production, proline accumulation and biofilm formation were good at 100 mM salt concentration with good microbial cell hydrophobicity at both 50 and 100 mM than other concentrations. Both the aggregated and non-aggregated cells grown under 0–200 mM salt concentrations produced IAA even at 200 mM salt concentration with a peak at 100 mM concentration with aggregated cells producing significantly higher quantities. ACC deaminase activity was observed in all NaCl concentrations studied with gradual and drastic reduction in aggregated and non-aggregated cells over increased salt concentrations.http://link.springer.com/article/10.1186/s13568-017-0518-7Methylobacterium oryzae CBMB20Salt stressC/N ratioProlinePHBEPS |
| spellingShingle | Mak Chanratana Gwang Hyun Han Aritra Roy Choudhury Seshadri Sundaram Md. Abdul Halim Ramasamy Krishnamoorthy Yeongyeong Kang Tongmin Sa Assessment of Methylobacterium oryzae CBMB20 aggregates for salt tolerance and plant growth promoting characteristics for bio-inoculant development AMB Express Methylobacterium oryzae CBMB20 Salt stress C/N ratio Proline PHB EPS |
| title | Assessment of Methylobacterium oryzae CBMB20 aggregates for salt tolerance and plant growth promoting characteristics for bio-inoculant development |
| title_full | Assessment of Methylobacterium oryzae CBMB20 aggregates for salt tolerance and plant growth promoting characteristics for bio-inoculant development |
| title_fullStr | Assessment of Methylobacterium oryzae CBMB20 aggregates for salt tolerance and plant growth promoting characteristics for bio-inoculant development |
| title_full_unstemmed | Assessment of Methylobacterium oryzae CBMB20 aggregates for salt tolerance and plant growth promoting characteristics for bio-inoculant development |
| title_short | Assessment of Methylobacterium oryzae CBMB20 aggregates for salt tolerance and plant growth promoting characteristics for bio-inoculant development |
| title_sort | assessment of methylobacterium oryzae cbmb20 aggregates for salt tolerance and plant growth promoting characteristics for bio inoculant development |
| topic | Methylobacterium oryzae CBMB20 Salt stress C/N ratio Proline PHB EPS |
| url | http://link.springer.com/article/10.1186/s13568-017-0518-7 |
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