Neutralizing Antibody Response to BBIBP-CorV in Comparison with COVID-19 Recovered, Unvaccinated Individuals in a Sample of the Pakistani Population
Fifty five percent of the Pakistani population is still unvaccinated with the two-dose protocol of COVID-19 vaccines. This study was undertaken to determine the seroconversion rate and antibody titers following the two-dose BBIBP-CorV protocol, and to compare these variables in unvaccinated, COVID-1...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/5/692 |
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| author | Javeria Aijaz Shakir Hussain Fouzia Naseer Fatima Kanani Sabiha Anis Samreen Sarfaraz Saima Saeed Hina Farooq Saba Jamal |
| author_facet | Javeria Aijaz Shakir Hussain Fouzia Naseer Fatima Kanani Sabiha Anis Samreen Sarfaraz Saima Saeed Hina Farooq Saba Jamal |
| author_sort | Javeria Aijaz |
| collection | DOAJ |
| description | Fifty five percent of the Pakistani population is still unvaccinated with the two-dose protocol of COVID-19 vaccines. This study was undertaken to determine the seroconversion rate and antibody titers following the two-dose BBIBP-CorV protocol, and to compare these variables in unvaccinated, COVID-19 recovered individuals (total <i>n</i> = 180) at Indus Hospital and Health Network, Karachi. Pseudotyped lentivirus antibody neutralization assays and SARS-CoV-2 IgG Quant II (Abbott) immunoassays were performed 4-8 weeks following the second dose of the BBIBP-CorV or PCR positivity/onset of symptoms of COVID-19. Seroconversion rate, using neutralization assays, in vaccinated individuals was lower (78%) than that in unvaccinated, COVID-19-recovered individuals with moderate to severe infection (97%). Prior PCR positivity increased serocoversion rate to 98% in vaccinated individuals. Immunoassays did not, however, reveal significant inter-group differences in seroconversion rates (≥95% in all groups). Log10 mean antibody neutralizing titers following the two-dose BBIBP-CorV protocol (IC50 = 2.21) were found to be significantly less than those succeeding moderate to severe COVID-19 (IC50 = 2.94). Prior SARS-CoV-2 positivity significantly increased post-vaccination antibody titers (IC50 = 2.82). Similar inter-group titer differences were obtained using the immunoassay. BBIBP-CorV post-vaccination titers may, thus, be lower than those following natural, moderate to severe infection, while prior SARS-CoV-2 exposure increases these titers to more closely approximate the latter. |
| first_indexed | 2024-03-10T01:40:13Z |
| format | Article |
| id | doaj.art-3ce10ee4a2324ee3b209ba81de04d27f |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T01:40:13Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-3ce10ee4a2324ee3b209ba81de04d27f2023-11-23T13:25:38ZengMDPI AGVaccines2076-393X2022-04-0110569210.3390/vaccines10050692Neutralizing Antibody Response to BBIBP-CorV in Comparison with COVID-19 Recovered, Unvaccinated Individuals in a Sample of the Pakistani PopulationJaveria Aijaz0Shakir Hussain1Fouzia Naseer2Fatima Kanani3Sabiha Anis4Samreen Sarfaraz5Saima Saeed6Hina Farooq7Saba Jamal8Molecular Biology Section, Pathology Department, Indus Hospital & Health Network, Karachi 75190, PakistanMolecular Biology Section, Pathology Department, Indus Hospital & Health Network, Karachi 75190, PakistanMolecular Biology Section, Pathology Department, Indus Hospital & Health Network, Karachi 75190, PakistanChemical Pathology Section, Pathology Department, Indus Hospital & Health Network, Karachi 75190, PakistanImmunology Section, Pathology Department, Indus Hospital & Health Network, Karachi 75190, PakistanInfectious Diseases Department, Indus Hospital & Health Network, Karachi 75190, PakistanPulmonology Department, Indus Hospital & Health Network, Karachi 75190, PakistanChemical Pathology Section, Pathology Department, Indus Hospital & Health Network, Karachi 75190, PakistanPathology Department, Indus Hospital & Health Network, Karachi 75190, PakistanFifty five percent of the Pakistani population is still unvaccinated with the two-dose protocol of COVID-19 vaccines. This study was undertaken to determine the seroconversion rate and antibody titers following the two-dose BBIBP-CorV protocol, and to compare these variables in unvaccinated, COVID-19 recovered individuals (total <i>n</i> = 180) at Indus Hospital and Health Network, Karachi. Pseudotyped lentivirus antibody neutralization assays and SARS-CoV-2 IgG Quant II (Abbott) immunoassays were performed 4-8 weeks following the second dose of the BBIBP-CorV or PCR positivity/onset of symptoms of COVID-19. Seroconversion rate, using neutralization assays, in vaccinated individuals was lower (78%) than that in unvaccinated, COVID-19-recovered individuals with moderate to severe infection (97%). Prior PCR positivity increased serocoversion rate to 98% in vaccinated individuals. Immunoassays did not, however, reveal significant inter-group differences in seroconversion rates (≥95% in all groups). Log10 mean antibody neutralizing titers following the two-dose BBIBP-CorV protocol (IC50 = 2.21) were found to be significantly less than those succeeding moderate to severe COVID-19 (IC50 = 2.94). Prior SARS-CoV-2 positivity significantly increased post-vaccination antibody titers (IC50 = 2.82). Similar inter-group titer differences were obtained using the immunoassay. BBIBP-CorV post-vaccination titers may, thus, be lower than those following natural, moderate to severe infection, while prior SARS-CoV-2 exposure increases these titers to more closely approximate the latter.https://www.mdpi.com/2076-393X/10/5/692BBIBP-CorVSinopharmCOVID-19Pakistanpseudotypedneutralizing |
| spellingShingle | Javeria Aijaz Shakir Hussain Fouzia Naseer Fatima Kanani Sabiha Anis Samreen Sarfaraz Saima Saeed Hina Farooq Saba Jamal Neutralizing Antibody Response to BBIBP-CorV in Comparison with COVID-19 Recovered, Unvaccinated Individuals in a Sample of the Pakistani Population Vaccines BBIBP-CorV Sinopharm COVID-19 Pakistan pseudotyped neutralizing |
| title | Neutralizing Antibody Response to BBIBP-CorV in Comparison with COVID-19 Recovered, Unvaccinated Individuals in a Sample of the Pakistani Population |
| title_full | Neutralizing Antibody Response to BBIBP-CorV in Comparison with COVID-19 Recovered, Unvaccinated Individuals in a Sample of the Pakistani Population |
| title_fullStr | Neutralizing Antibody Response to BBIBP-CorV in Comparison with COVID-19 Recovered, Unvaccinated Individuals in a Sample of the Pakistani Population |
| title_full_unstemmed | Neutralizing Antibody Response to BBIBP-CorV in Comparison with COVID-19 Recovered, Unvaccinated Individuals in a Sample of the Pakistani Population |
| title_short | Neutralizing Antibody Response to BBIBP-CorV in Comparison with COVID-19 Recovered, Unvaccinated Individuals in a Sample of the Pakistani Population |
| title_sort | neutralizing antibody response to bbibp corv in comparison with covid 19 recovered unvaccinated individuals in a sample of the pakistani population |
| topic | BBIBP-CorV Sinopharm COVID-19 Pakistan pseudotyped neutralizing |
| url | https://www.mdpi.com/2076-393X/10/5/692 |
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