Isolation and Identification of Bacteria with Surface and Antibacterial Activity from the Gut of Mediterranean Grey Mullets
Fish gut represents a peculiar ecological niche where bacteria can transit and reside to play vital roles by producing bio-compounds with nutritional, immunomodulatory and other functions. This complex microbial ecosystem reflects several factors (environment, feeding regimen, fish species, etc.). T...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/12/2555 |
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| author | Rosanna Floris Gabriele Sanna Laura Mura Myriam Fiori Jacopo Culurgioni Riccardo Diciotti Carmen Rizzo Angelina Lo Giudice Pasqualina Laganà Nicola Fois |
| author_facet | Rosanna Floris Gabriele Sanna Laura Mura Myriam Fiori Jacopo Culurgioni Riccardo Diciotti Carmen Rizzo Angelina Lo Giudice Pasqualina Laganà Nicola Fois |
| author_sort | Rosanna Floris |
| collection | DOAJ |
| description | Fish gut represents a peculiar ecological niche where bacteria can transit and reside to play vital roles by producing bio-compounds with nutritional, immunomodulatory and other functions. This complex microbial ecosystem reflects several factors (environment, feeding regimen, fish species, etc.). The objective of the present study was the identification of intestinal microbial strains able to produce molecules called biosurfactants (BSs), which were tested for surface and antibacterial activity in order to select a group of probiotic bacteria for aquaculture use. Forty-two bacterial isolates from the digestive tracts of twenty Mediterranean grey mullets were screened for testing emulsifying (E-24), surface and antibiotic activities. Fifty percent of bacteria, ascribed to <i>Pseudomonas aeruginosa</i>, <i>Pseudomonas</i> sp., <i>P. putida</i> and <i>P. anguilliseptica, P. stutzeri, P. protegens</i> and <i>Enterobacter ludwigii</i> were found to be surfactant producers. Of the tested strains, 26.6% exhibited an antibacterial activity against <i>Staphylococcus aureus</i> (10.0 ± 0.0–14.5 ± 0.7 mm inhibition zone), and among them, 23.3% of isolates also showed inhibitory activity vs. <i>Proteus mirabilis</i> (10.0 ± 0.0–18.5 ± 0.7 mm inhibition zone) and 6.6% vs. <i>Klebsiella pneumoniae</i> (11.5 ± 0.7–17.5 ± 0.7 mm inhibition zone). According to preliminary chemical analysis, the bioactive compounds are suggested to be ascribed to the class of glycolipids. This works indicated that fish gut is a source of bioactive compounds which deserves to be explored. |
| first_indexed | 2024-03-10T03:31:10Z |
| format | Article |
| id | doaj.art-3ce29f803d9743198623bcd11ece03c7 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T03:31:10Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3ce29f803d9743198623bcd11ece03c72023-11-23T09:39:45ZengMDPI AGMicroorganisms2076-26072021-12-01912255510.3390/microorganisms9122555Isolation and Identification of Bacteria with Surface and Antibacterial Activity from the Gut of Mediterranean Grey MulletsRosanna Floris0Gabriele Sanna1Laura Mura2Myriam Fiori3Jacopo Culurgioni4Riccardo Diciotti5Carmen Rizzo6Angelina Lo Giudice7Pasqualina Laganà8Nicola Fois9AGRIS-Sardegna, Agricultural Research Agency of Sardinia, Bonassai, 07100 Sassari, ItalyAGRIS-Sardegna, Agricultural Research Agency of Sardinia, Bonassai, 07100 Sassari, ItalyAGRIS-Sardegna, Agricultural Research Agency of Sardinia, Bonassai, 07100 Sassari, ItalyAGRIS-Sardegna, Agricultural Research Agency of Sardinia, Bonassai, 07100 Sassari, ItalyAGRIS-Sardegna, Agricultural Research Agency of Sardinia, Bonassai, 07100 Sassari, ItalyAGRIS-Sardegna, Agricultural Research Agency of Sardinia, Bonassai, 07100 Sassari, ItalyStazione Zoologica Anton Dohrn-Ecosustainable Marine Biotechnology Department, Sicily Marine Centre, Villa Pace, Contrada Porticatello 29, 98167 Messina, ItalyInstitute of Polar Sciences, National Research Council (ISP-CNR), 98122 Messina, ItalyDepartment of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, Torre Biologica 3p, AOU ‘G. Martino, Via C. Valeria, s.n.c., 98125 Messina, ItalyAGRIS-Sardegna, Agricultural Research Agency of Sardinia, Bonassai, 07100 Sassari, ItalyFish gut represents a peculiar ecological niche where bacteria can transit and reside to play vital roles by producing bio-compounds with nutritional, immunomodulatory and other functions. This complex microbial ecosystem reflects several factors (environment, feeding regimen, fish species, etc.). The objective of the present study was the identification of intestinal microbial strains able to produce molecules called biosurfactants (BSs), which were tested for surface and antibacterial activity in order to select a group of probiotic bacteria for aquaculture use. Forty-two bacterial isolates from the digestive tracts of twenty Mediterranean grey mullets were screened for testing emulsifying (E-24), surface and antibiotic activities. Fifty percent of bacteria, ascribed to <i>Pseudomonas aeruginosa</i>, <i>Pseudomonas</i> sp., <i>P. putida</i> and <i>P. anguilliseptica, P. stutzeri, P. protegens</i> and <i>Enterobacter ludwigii</i> were found to be surfactant producers. Of the tested strains, 26.6% exhibited an antibacterial activity against <i>Staphylococcus aureus</i> (10.0 ± 0.0–14.5 ± 0.7 mm inhibition zone), and among them, 23.3% of isolates also showed inhibitory activity vs. <i>Proteus mirabilis</i> (10.0 ± 0.0–18.5 ± 0.7 mm inhibition zone) and 6.6% vs. <i>Klebsiella pneumoniae</i> (11.5 ± 0.7–17.5 ± 0.7 mm inhibition zone). According to preliminary chemical analysis, the bioactive compounds are suggested to be ascribed to the class of glycolipids. This works indicated that fish gut is a source of bioactive compounds which deserves to be explored.https://www.mdpi.com/2076-2607/9/12/2555intestinal microflorafish gutbiosurfactantsgrey mulletsnatural antibiotics |
| spellingShingle | Rosanna Floris Gabriele Sanna Laura Mura Myriam Fiori Jacopo Culurgioni Riccardo Diciotti Carmen Rizzo Angelina Lo Giudice Pasqualina Laganà Nicola Fois Isolation and Identification of Bacteria with Surface and Antibacterial Activity from the Gut of Mediterranean Grey Mullets Microorganisms intestinal microflora fish gut biosurfactants grey mullets natural antibiotics |
| title | Isolation and Identification of Bacteria with Surface and Antibacterial Activity from the Gut of Mediterranean Grey Mullets |
| title_full | Isolation and Identification of Bacteria with Surface and Antibacterial Activity from the Gut of Mediterranean Grey Mullets |
| title_fullStr | Isolation and Identification of Bacteria with Surface and Antibacterial Activity from the Gut of Mediterranean Grey Mullets |
| title_full_unstemmed | Isolation and Identification of Bacteria with Surface and Antibacterial Activity from the Gut of Mediterranean Grey Mullets |
| title_short | Isolation and Identification of Bacteria with Surface and Antibacterial Activity from the Gut of Mediterranean Grey Mullets |
| title_sort | isolation and identification of bacteria with surface and antibacterial activity from the gut of mediterranean grey mullets |
| topic | intestinal microflora fish gut biosurfactants grey mullets natural antibiotics |
| url | https://www.mdpi.com/2076-2607/9/12/2555 |
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