Comparison of Multiple Strategies for Precision Transgene Knock-In in <i>Gallus gallus</i> Genome via Microhomology-Mediated End Joining

Comparison of Multiple Strategies for Precision Transgene Knock-In in <i>Gallus gallus</i> Genome via Microhomology-Mediated End Joining

Precision exogenous gene knock-in is an attractive field for transgenic <i>Gallus gallus</i> (chicken) generation. In this article, we constructed multiple Precise Integration into Target Chromosome (PITCh) plasmid systems mediated by microhomology-mediated end-joining (MMEJ) for large-f...

Full description

Bibliographic Details
Main Authors:	Lijuan Wang, Jiaxin Sun, Zhipeng Liu, Qiang Zheng, Guojun Wang
Format:	Article
Language:	English
Published:	MDPI AG 2023-10-01
Series:	International Journal of Molecular Sciences
Subjects:	MMEJ <i>GAPDH</i> DF-1 cell line precise integration CRISPR/Cas9 safe harbor loci
Online Access:	https://www.mdpi.com/1422-0067/24/21/15731

Similar Items

Integrating Omics and CRISPR Technology for Identification and Verification of Genomic Safe Harbor Loci in the Chicken Genome
by: Nima Dehdilani, et al.
Published: (2023-06-01)

The collagen type I alpha 1 chain gene is an alternative safe harbor locus in the porcine genome
by: Guang-ming XIANG, et al.
Published: (2023-01-01)

Identification of the <i>CKM</i> Gene as a Potential Muscle-Specific Safe Harbor Locus in Pig Genome
by: Youcai Xiong, et al.
Published: (2022-05-01)

Quantification of transgene expression in GSH AAVS1 with a novel CRISPR/Cas9-based approach reveals high transcriptional variation
by: Anne Inderbitzin, et al.
Published: (2022-09-01)

Endogenous CRISPR-assisted microhomology-mediated end joining enables rapid genome editing in Zymomonas mobilis
by: Xin Sui, et al.
Published: (2021-10-01)

Contribution of Microhomology to Genome Instability: Connection between DNA Repair and Replication Stress
by: Yuning Jiang
Published: (2022-10-01)

<i>Spexin2</i> Is a Novel Food Regulator in <i>Gallus gallus</i>
by: Fengyan Meng, et al.
Published: (2023-03-01)

Enhancing stability of recombinant CHO cells by CRISPR/Cas9-mediated site-specific integration into regions with distinct histone modifications
by: Oliver Hertel, et al.
Published: (2022-10-01)

Correlation between Microsatellite Loci and Onset of Lay and Egg Quality Traits in Chinese Silkies, Gallus gallus
by: Yan Zhou, et al.
Published: (2008-10-01)

Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (<i>Gallus gallus</i>)
by: Kai Jin, et al.
Published: (2020-11-01)

Beta human papillomavirus 8E6 promotes alternative end joining
by: Changkun Hu, et al.
Published: (2023-01-01)

ErCas12a and T5exo-ErCas12a Mediate Simple and Efficient Genome Editing in Zebrafish
by: Bingzhou Han, et al.
Published: (2022-03-01)

Intra-Amniotic Administration—An Emerging Method to Investigate Necrotizing Enterocolitis, In Vivo (<i>Gallus gallus</i>)
by: Nikolai Kolba, et al.
Published: (2022-11-01)

Knocking Out <i>OsAAP11</i> to Improve Rice Grain Quality Using CRISPR/Cas9 System
by: Yihao Yang, et al.
Published: (2023-09-01)

Morphological and Molecular Characterization of <i>Eimeria</i> spp. Infecting Domestic Poultry <i>Gallus gallus</i> in Riyadh City, Saudi Arabia
by: Mohammed M. Mares, et al.
Published: (2023-03-01)

Prevalence and Diversity of Blood Parasites (<i>Plasmodium</i>, <i>Leucocytozoon</i> and <i>Trypanosoma</i>) in Backyard Chickens (<i>Gallus gallus domesticus</i>) Raised in Southern Thailand
by: Kanpapat Boonchuay, et al.
Published: (2023-09-01)

Comprehensive Mining and Characterization of CRISPR-Cas Systems in <i>Bifidobacterium</i>
by: Meichen Pan, et al.
Published: (2020-05-01)

Improving Expression of Pepsinogen A from <i>Homo sapiens</i> in <i>Aspergillus niger</i> by Using a Multi-Copy Gene Knock-in Strategy
by: Jie Chen, et al.
Published: (2023-05-01)

Intra-Amniotic Administration of Cashew Nut (<i>Anacardium occidentale</i> L.) Soluble Extract Improved Gut Functionality and Morphology In Vivo (<i>Gallus gallus</i>)
by: Talitha Silva Meneguelli, et al.
Published: (2023-05-01)

Towards Tabula Gallus
by: Masahito Yamagata
Published: (2022-01-01)

New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i
by: Jérémy Villette, et al.
Published: (2024-03-01)

Population Structure and Genetic Diversity of <i>Colletotrichum gloeosporioides</i> on <i>Citrus</i> in China
by: Bei Liu, et al.
Published: (2023-01-01)

CRISPR-Cas9: A Powerful Tool to Efficiently Engineer <i>Saccharomyces cerevisiae</i>
by: João Rainha, et al.
Published: (2020-12-01)

Kerapatan Jenis Ayam Hutan Merah (Gallus Gallus) Dan Ayam Hutan Hijau (Gallus Varius) Di Hutan Musim Taman Nasional Baluran
by: Perpustakaan UGM, i-lib
Published: (2001)

<i>Aspergillus</i> spp. Isolated from Lungs of Poultry (<i>Gallus gallus</i>) at the Mycology Laboratory, School of Veterinary Medicine, Universidad Nacional, Heredia, Costa Rica between 2008 and 2021 and Associated Factors
by: Oscar Ulloa-Avellán, et al.
Published: (2022-12-01)

Editing of a Novel Cd Uptake-Related Gene <i>CUP1</i> Contributes to Reducing Cd Accumulations in <i>Arabidopsis thaliana</i> and <i>Brassica napus</i>
by: Junyu Yao, et al.
Published: (2022-12-01)

Knocking Out Chloroplastic Aldolases/Rubisco Lysine Methyltransferase Enhances Biomass Accumulation in <i>Nannochloropsis oceanica</i> under High-Light Stress
by: Wensi Liang, et al.
Published: (2024-03-01)

Empire Apple (<i>Malus domestica</i>) Juice, Pomace, and Pulp Modulate Intestinal Functionality, Morphology, and Bacterial Populations In Vivo (<i>Gallus gallus</i>)
by: Cydney Jackson, et al.
Published: (2022-11-01)

Pheromone Receptor Knock-Out Affects Pheromone Detection and Brain Structure in a Moth
by: Fotini Koutroumpa, et al.
Published: (2022-02-01)

Chia Phenolic Extract Appear to Improve Small Intestinal Functionality, Morphology, Bacterial Populations, and Inflammation Biomarkers In Vivo (<i>Gallus gallus</i>)
by: Marcella Duarte Villas Mishima, et al.
Published: (2023-08-01)

CRISPR/Cas9-Based Knock-Out of the <i>PMR4</i> Gene Reduces Susceptibility to Late Blight in Two Tomato Cultivars
by: Ruiling Li, et al.
Published: (2022-11-01)

Saffron (<i>Crocus sativus</i> L.) Flower Water Extract Disrupts the Cecal Microbiome, Brush Border Membrane Functionality, and Morphology In Vivo (<i>Gallus gallus</i>)
by: Nikita Agarwal, et al.
Published: (2022-01-01)

Efficient Genetic Safety Switches for Future Application of iPSC-Derived Cell Transplants
by: Julia Dahlke, et al.
Published: (2021-06-01)

Exploring the Interplay of the CRISPR-CAS System with Antibiotic Resistance in <i>Staphylococcus aureus</i>: A Poultry Meat Study from Lahore, Pakistan
by: Muhammad Abu Bakr Shabbir, et al.
Published: (2024-01-01)

Multi-Gene Phylogenetic Approach for Identification and Diversity Analysis of <i>Bipolaris maydis</i> and <i>Curvularia lunata</i> Isolates Causing Foliar Blight of <i>Zea mays</i>
by: Nazia Manzar, et al.
Published: (2022-07-01)

Plant DNA Repair and <i>Agrobacterium</i> T−DNA Integration
by: Stanton B. Gelvin
Published: (2021-08-01)

Development and Applications of CRISPR/Cas9-Based Genome Editing in <i>Lactobacillus</i>
by: Yulin Mu, et al.
Published: (2022-10-01)

Regulation of CRISPR-Associated Genes by Rv1776c (CasR) in <i>Mycobacterium tuberculosis</i>
by: Wenping Wei, et al.
Published: (2023-02-01)

Prevalence of endoparasites in village chicken (Gallus gallus domesticus) and wild jungle fowl (Gallus gallus spadiceus).
by: Reduan, Mazleen Laili, et al.
Published: (2010)

Circadian Corticosterone Profile in Laying Hens (<i>Gallus gallus domesticus</i>)
by: Theresa Hillebrecht, et al.
Published: (2024-03-01)