Pharmacokinetic comparison of four major bio-active components of naoxintong capsule in normal and acute blood stasis rats using ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry
Objective: To compare the pharmacokinetic differences of the main components of Naoxintong capsule (NXTC) in normal and acute blood stasis rats. Materials and Methods: Rats were subcutaneously injected with adrenaline hydrochloride twice; during the two subcutaneous injections, the rats were placed...
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| Format: | Article |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | World Journal of Traditional Chinese Medicine |
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| Online Access: | http://www.wjtcm.net/article.asp?issn=2311-8571;year=2022;volume=8;issue=1;spage=92;epage=99;aulast=Li |
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| author | Wei-Xia Li Shu-Qi Zhang Man-Man Li Hui Zhang Xiao-Yan Wang Lu Niu Jin-Fa Tang Xue-Lin Li |
| author_facet | Wei-Xia Li Shu-Qi Zhang Man-Man Li Hui Zhang Xiao-Yan Wang Lu Niu Jin-Fa Tang Xue-Lin Li |
| author_sort | Wei-Xia Li |
| collection | DOAJ |
| description | Objective: To compare the pharmacokinetic differences of the main components of Naoxintong capsule (NXTC) in normal and acute blood stasis rats. Materials and Methods: Rats were subcutaneously injected with adrenaline hydrochloride twice; during the two subcutaneous injections, the rats were placed in ice water for 4 min to reproduce the model rat of acute blood stasis. The normal and acute blood stasis rats were administrated a 5.04 g/kg dose of NXTC suspension. Then, blood samples were collected from the posterior retinal venous plexus at different time points. Plasma concentrations of four major bio-active components including caffeic acid, ferulic acid, formononetin, and tanshinone IIA in NXTC were measured using ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry. Phoenix WinNonlin v6.2 software was used to calculate the pharmacokinetic parameters. Results: Compared with the normal rats, the acute blood stasis rats showed a significant decrease in Cmax of ferulic acid and formononetin, AUCall of caffeic acid and ferulic acid, and AUCINF_obs of ferulic acid. Conversely, an increase in the Vz_F_obs and MRTlast of ferulic acid and caffeic acid was observed. These findings demonstrate that the absorption of the four NXTC components was weakened in the acute blood stasis rats and that the elimination time was prolonged. Conclusions: The significant difference in some parameters of the four NXTC components between the normal and acute blood stasis rats might be caused by an increase in blood viscosity and the subsequent slowing down of blood flow in the acute blood stasis rats. The pharmacokinetic study conducted in pathological state can provide important information and scientific basis for further rational clinical application of NXTC. |
| first_indexed | 2024-04-11T17:59:33Z |
| format | Article |
| id | doaj.art-3ce2f3c2566e41bb81797b2cc63022fc |
| institution | Directory Open Access Journal |
| issn | 2311-8571 |
| language | English |
| last_indexed | 2024-04-11T17:59:33Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | World Journal of Traditional Chinese Medicine |
| spelling | doaj.art-3ce2f3c2566e41bb81797b2cc63022fc2022-12-22T04:10:34ZengWolters Kluwer Medknow PublicationsWorld Journal of Traditional Chinese Medicine2311-85712022-01-0181929910.4103/wjtcm.wjtcm_53_21Pharmacokinetic comparison of four major bio-active components of naoxintong capsule in normal and acute blood stasis rats using ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometryWei-Xia LiShu-Qi ZhangMan-Man LiHui ZhangXiao-Yan WangLu NiuJin-Fa TangXue-Lin LiObjective: To compare the pharmacokinetic differences of the main components of Naoxintong capsule (NXTC) in normal and acute blood stasis rats. Materials and Methods: Rats were subcutaneously injected with adrenaline hydrochloride twice; during the two subcutaneous injections, the rats were placed in ice water for 4 min to reproduce the model rat of acute blood stasis. The normal and acute blood stasis rats were administrated a 5.04 g/kg dose of NXTC suspension. Then, blood samples were collected from the posterior retinal venous plexus at different time points. Plasma concentrations of four major bio-active components including caffeic acid, ferulic acid, formononetin, and tanshinone IIA in NXTC were measured using ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry. Phoenix WinNonlin v6.2 software was used to calculate the pharmacokinetic parameters. Results: Compared with the normal rats, the acute blood stasis rats showed a significant decrease in Cmax of ferulic acid and formononetin, AUCall of caffeic acid and ferulic acid, and AUCINF_obs of ferulic acid. Conversely, an increase in the Vz_F_obs and MRTlast of ferulic acid and caffeic acid was observed. These findings demonstrate that the absorption of the four NXTC components was weakened in the acute blood stasis rats and that the elimination time was prolonged. Conclusions: The significant difference in some parameters of the four NXTC components between the normal and acute blood stasis rats might be caused by an increase in blood viscosity and the subsequent slowing down of blood flow in the acute blood stasis rats. The pharmacokinetic study conducted in pathological state can provide important information and scientific basis for further rational clinical application of NXTC.http://www.wjtcm.net/article.asp?issn=2311-8571;year=2022;volume=8;issue=1;spage=92;epage=99;aulast=Liacute blood stasis rat model; naoxintong capsules; pharmacokinetics; ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry |
| spellingShingle | Wei-Xia Li Shu-Qi Zhang Man-Man Li Hui Zhang Xiao-Yan Wang Lu Niu Jin-Fa Tang Xue-Lin Li Pharmacokinetic comparison of four major bio-active components of naoxintong capsule in normal and acute blood stasis rats using ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry World Journal of Traditional Chinese Medicine acute blood stasis rat model; naoxintong capsules; pharmacokinetics; ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry |
| title | Pharmacokinetic comparison of four major bio-active components of naoxintong capsule in normal and acute blood stasis rats using ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry |
| title_full | Pharmacokinetic comparison of four major bio-active components of naoxintong capsule in normal and acute blood stasis rats using ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry |
| title_fullStr | Pharmacokinetic comparison of four major bio-active components of naoxintong capsule in normal and acute blood stasis rats using ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry |
| title_full_unstemmed | Pharmacokinetic comparison of four major bio-active components of naoxintong capsule in normal and acute blood stasis rats using ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry |
| title_short | Pharmacokinetic comparison of four major bio-active components of naoxintong capsule in normal and acute blood stasis rats using ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry |
| title_sort | pharmacokinetic comparison of four major bio active components of naoxintong capsule in normal and acute blood stasis rats using ultra performance liquid chromatography coupled with triple quadrupole mass spectrometry |
| topic | acute blood stasis rat model; naoxintong capsules; pharmacokinetics; ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry |
| url | http://www.wjtcm.net/article.asp?issn=2311-8571;year=2022;volume=8;issue=1;spage=92;epage=99;aulast=Li |
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