Light-Induced Vitamin C Accumulation in Tomato Fruits is Independent of Carbohydrate Availability
L-ascorbate (ASC) is essential for human health. Therefore, there is interest in increasing the ASC content of crops like tomato. High irradiance induces accumulation of ASC in green tomato fruits. The D-mannose/L-galactose biosynthetic pathway accounts for the most ASC in plants. The myo-inositol a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/8/4/86 |
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| author | Nikolaos Ntagkas Ernst Woltering Sofoklis Bouras Ric C. H. de Vos J Anja Dieleman Celine C. S. Nicole Caroline Labrie Leo F. M. Marcelis |
| author_facet | Nikolaos Ntagkas Ernst Woltering Sofoklis Bouras Ric C. H. de Vos J Anja Dieleman Celine C. S. Nicole Caroline Labrie Leo F. M. Marcelis |
| author_sort | Nikolaos Ntagkas |
| collection | DOAJ |
| description | L-ascorbate (ASC) is essential for human health. Therefore, there is interest in increasing the ASC content of crops like tomato. High irradiance induces accumulation of ASC in green tomato fruits. The D-mannose/L-galactose biosynthetic pathway accounts for the most ASC in plants. The myo-inositol and galacturonate pathways have been proposed to exist but never identified in plants. The D-mannose/L-galactose starts from D-glucose. In a series of experiments, we tested the hypothesis that ASC levels depend on soluble carbohydrate content when tomato fruits ripen under irradiances that stimulate ASC biosynthesis. We show that ASC levels considerably increased when fruits ripened under light, but carbohydrate levels did not show a parallel increase. When carbohydrate levels in fruits were altered by flower pruning, no effects on ASC levels were observed at harvest or after ripening under irradiances that induce ASC accumulation. Artificial feeding of trusses with sucrose increased carbohydrate levels, but did not affect the light-induced ASC levels. We conclude that light-induced accumulation of ASC is independent of the carbohydrate content in tomato fruits. In tomato fruit treated with light, the increase in ASC was preceded by a concomitant increase in myo-inositol. |
| first_indexed | 2024-12-13T10:43:46Z |
| format | Article |
| id | doaj.art-3ce4325503de4a2d926d0f55bec25bc7 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-12-13T10:43:46Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-3ce4325503de4a2d926d0f55bec25bc72022-12-21T23:50:19ZengMDPI AGPlants2223-77472019-04-01848610.3390/plants8040086plants8040086Light-Induced Vitamin C Accumulation in Tomato Fruits is Independent of Carbohydrate AvailabilityNikolaos Ntagkas0Ernst Woltering1Sofoklis Bouras2Ric C. H. de Vos3J Anja Dieleman4Celine C. S. Nicole5Caroline Labrie6Leo F. M. Marcelis7Horticulture and Product Physiology, Wageningen University and Research, Droevendaalsesteeg 1, 6709 PB Wageningen, The NetherlandsHorticulture and Product Physiology, Wageningen University and Research, Droevendaalsesteeg 1, 6709 PB Wageningen, The NetherlandsHorticulture and Product Physiology, Wageningen University and Research, Droevendaalsesteeg 1, 6709 PB Wageningen, The NetherlandsBusiness unit Bioscience, Wageningen University and Research, Droevendaalsesteeg 1, 6709 PB Wageningen, The NetherlandsBusiness unit Greenhouse Horticulture, Wageningen University and Research, Violierenweg 1, 2665 MV Bleiswijk, The NetherlandsSignify Research, High Tech Campus 7, 5656 AE Eindhoven, The NetherlandsBusiness unit Greenhouse Horticulture, Wageningen University and Research, Violierenweg 1, 2665 MV Bleiswijk, The NetherlandsHorticulture and Product Physiology, Wageningen University and Research, Droevendaalsesteeg 1, 6709 PB Wageningen, The NetherlandsL-ascorbate (ASC) is essential for human health. Therefore, there is interest in increasing the ASC content of crops like tomato. High irradiance induces accumulation of ASC in green tomato fruits. The D-mannose/L-galactose biosynthetic pathway accounts for the most ASC in plants. The myo-inositol and galacturonate pathways have been proposed to exist but never identified in plants. The D-mannose/L-galactose starts from D-glucose. In a series of experiments, we tested the hypothesis that ASC levels depend on soluble carbohydrate content when tomato fruits ripen under irradiances that stimulate ASC biosynthesis. We show that ASC levels considerably increased when fruits ripened under light, but carbohydrate levels did not show a parallel increase. When carbohydrate levels in fruits were altered by flower pruning, no effects on ASC levels were observed at harvest or after ripening under irradiances that induce ASC accumulation. Artificial feeding of trusses with sucrose increased carbohydrate levels, but did not affect the light-induced ASC levels. We conclude that light-induced accumulation of ASC is independent of the carbohydrate content in tomato fruits. In tomato fruit treated with light, the increase in ASC was preceded by a concomitant increase in myo-inositol.https://www.mdpi.com/2223-7747/8/4/86vitamin Cascorbic acidcarbohydratesirradiancemyo-inositolgalacturonate |
| spellingShingle | Nikolaos Ntagkas Ernst Woltering Sofoklis Bouras Ric C. H. de Vos J Anja Dieleman Celine C. S. Nicole Caroline Labrie Leo F. M. Marcelis Light-Induced Vitamin C Accumulation in Tomato Fruits is Independent of Carbohydrate Availability Plants vitamin C ascorbic acid carbohydrates irradiance myo-inositol galacturonate |
| title | Light-Induced Vitamin C Accumulation in Tomato Fruits is Independent of Carbohydrate Availability |
| title_full | Light-Induced Vitamin C Accumulation in Tomato Fruits is Independent of Carbohydrate Availability |
| title_fullStr | Light-Induced Vitamin C Accumulation in Tomato Fruits is Independent of Carbohydrate Availability |
| title_full_unstemmed | Light-Induced Vitamin C Accumulation in Tomato Fruits is Independent of Carbohydrate Availability |
| title_short | Light-Induced Vitamin C Accumulation in Tomato Fruits is Independent of Carbohydrate Availability |
| title_sort | light induced vitamin c accumulation in tomato fruits is independent of carbohydrate availability |
| topic | vitamin C ascorbic acid carbohydrates irradiance myo-inositol galacturonate |
| url | https://www.mdpi.com/2223-7747/8/4/86 |
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