Identification of active natural products that induce lysosomal biogenesis by lysosome-based screening and biological evaluation
Lysosomal biogenesis is an essential adaptive process by which lysosomes exert their function in maintaining cellular homeostasis. Defects in lysosomal enzymes and functions lead to lysosome-related diseases, including lysosomal storage diseases and neurodegenerative disorders. Thus, activation of t...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022024677 |
| _version_ | 1797991613026271232 |
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| author | Xiao Ding Xu Yang Yueqin Zhao Yinyuan Wang Jimin Fei Zhenpeng Niu Xianxiang Dong Xuenan Wang Biao Liu Hongmei Li Xiaojiang Hao Yuhan Zhao |
| author_facet | Xiao Ding Xu Yang Yueqin Zhao Yinyuan Wang Jimin Fei Zhenpeng Niu Xianxiang Dong Xuenan Wang Biao Liu Hongmei Li Xiaojiang Hao Yuhan Zhao |
| author_sort | Xiao Ding |
| collection | DOAJ |
| description | Lysosomal biogenesis is an essential adaptive process by which lysosomes exert their function in maintaining cellular homeostasis. Defects in lysosomal enzymes and functions lead to lysosome-related diseases, including lysosomal storage diseases and neurodegenerative disorders. Thus, activation of the autophagy-lysosomal pathway, especially induction of lysosomal biogenesis, might be an effective strategy for the treatment of lysosome-related diseases. In this study, we established a lysosome-based screening system to identify active compounds from natural products that could promote lysosomal biogenesis. The subcellular localizations of master transcriptional regulators of lysosomal genes, TFEB, TFE3 and ZKSCAN3 were examined to reveal the potential mechanisms. More than 200 compounds were screened, and we found that Hdj-23, a triterpene isolated from Walsura cochinchinensis, induced lysosomal biogenesis via activation of TFEB/TFE3. In summary, this study introduced a lysosome-based live cell screening strategy to identify bioactive compounds that promote lysosomal biogenesis, which would provide potential candidate enhancers of lysosomal biogenesis and novel insight for treating lysosome-related diseases. |
| first_indexed | 2024-04-11T08:54:59Z |
| format | Article |
| id | doaj.art-3ce6f0511eed477b97e67f2159187817 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-11T08:54:59Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-3ce6f0511eed477b97e67f21591878172022-12-22T04:33:20ZengElsevierHeliyon2405-84402022-10-01810e11179Identification of active natural products that induce lysosomal biogenesis by lysosome-based screening and biological evaluationXiao Ding0Xu Yang1Yueqin Zhao2Yinyuan Wang3Jimin Fei4Zhenpeng Niu5Xianxiang Dong6Xuenan Wang7Biao Liu8Hongmei Li9Xiaojiang Hao10Yuhan Zhao11State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, PR China; Research Unit of Chemical Biology of Natural Anti-Virus Products, Chinese Academy of Medical Sciences, Beijing 100730, PR China; Corresponding author.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR ChinaState Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR ChinaState Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, PR China; School of Life Sciences, Yunnan University, Kunming 650091, Yunnan, PR ChinaYunnan Cancer Hospital & The Third Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan, PR ChinaState Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, PR China; School of Basic Medicine, Guizhou Medical University, Guiyang 550009, Guizhou, PR ChinaState Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR ChinaState Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, PR China; Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, PR ChinaYunnan Cancer Hospital & The Third Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan, PR ChinaState Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, PR ChinaState Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, PR China; Research Unit of Chemical Biology of Natural Anti-Virus Products, Chinese Academy of Medical Sciences, Beijing 100730, PR China; Corresponding author.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, PR China; Corresponding author.Lysosomal biogenesis is an essential adaptive process by which lysosomes exert their function in maintaining cellular homeostasis. Defects in lysosomal enzymes and functions lead to lysosome-related diseases, including lysosomal storage diseases and neurodegenerative disorders. Thus, activation of the autophagy-lysosomal pathway, especially induction of lysosomal biogenesis, might be an effective strategy for the treatment of lysosome-related diseases. In this study, we established a lysosome-based screening system to identify active compounds from natural products that could promote lysosomal biogenesis. The subcellular localizations of master transcriptional regulators of lysosomal genes, TFEB, TFE3 and ZKSCAN3 were examined to reveal the potential mechanisms. More than 200 compounds were screened, and we found that Hdj-23, a triterpene isolated from Walsura cochinchinensis, induced lysosomal biogenesis via activation of TFEB/TFE3. In summary, this study introduced a lysosome-based live cell screening strategy to identify bioactive compounds that promote lysosomal biogenesis, which would provide potential candidate enhancers of lysosomal biogenesis and novel insight for treating lysosome-related diseases.http://www.sciencedirect.com/science/article/pii/S2405844022024677Lysosomal biogenesisNatural productsTFEB and TFE3Lysosome-based screeningAutophagy |
| spellingShingle | Xiao Ding Xu Yang Yueqin Zhao Yinyuan Wang Jimin Fei Zhenpeng Niu Xianxiang Dong Xuenan Wang Biao Liu Hongmei Li Xiaojiang Hao Yuhan Zhao Identification of active natural products that induce lysosomal biogenesis by lysosome-based screening and biological evaluation Heliyon Lysosomal biogenesis Natural products TFEB and TFE3 Lysosome-based screening Autophagy |
| title | Identification of active natural products that induce lysosomal biogenesis by lysosome-based screening and biological evaluation |
| title_full | Identification of active natural products that induce lysosomal biogenesis by lysosome-based screening and biological evaluation |
| title_fullStr | Identification of active natural products that induce lysosomal biogenesis by lysosome-based screening and biological evaluation |
| title_full_unstemmed | Identification of active natural products that induce lysosomal biogenesis by lysosome-based screening and biological evaluation |
| title_short | Identification of active natural products that induce lysosomal biogenesis by lysosome-based screening and biological evaluation |
| title_sort | identification of active natural products that induce lysosomal biogenesis by lysosome based screening and biological evaluation |
| topic | Lysosomal biogenesis Natural products TFEB and TFE3 Lysosome-based screening Autophagy |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022024677 |
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