Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets
The effects of the dietary administration of a combination of Quebracho and Chestnut tannins, leonardite and tributyrin were evaluated in weaned piglets. A total of 168 weaned piglets (Landrace × Large White) were randomly allotted to two experimental groups (6 pens/group, 14 piglets/pen). Animals w...
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MDPI AG
2021-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/6/1693 |
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| author | Matteo Dell’Anno Serena Reggi Valentina Caprarulo Monika Hejna Carlo Angelo Sgoifo Rossi Maria Luisa Callegari Antonella Baldi Luciana Rossi |
| author_facet | Matteo Dell’Anno Serena Reggi Valentina Caprarulo Monika Hejna Carlo Angelo Sgoifo Rossi Maria Luisa Callegari Antonella Baldi Luciana Rossi |
| author_sort | Matteo Dell’Anno |
| collection | DOAJ |
| description | The effects of the dietary administration of a combination of Quebracho and Chestnut tannins, leonardite and tributyrin were evaluated in weaned piglets. A total of 168 weaned piglets (Landrace × Large White) were randomly allotted to two experimental groups (6 pens/group, 14 piglets/pen). Animals were fed a basal control diet (CTRL) and a treatment diet (MIX) supplemented with 0.75% tannin extracts, 0.25% leonardite and 0.20% tributyrin for 28 days. Individual body weight and feed intake were recorded weekly. Diarrhoea incidence was recorded by a faecal scoring scale (0–3; considering diarrhoea ≥ 2). At 0 and 28 days, faecal samples were obtained from four piglets/pen for microbiological and chemical analyses of faecal microbiota, which were then assessed by V3-V4 region amplification sequencing. At 28 days, blood from two piglets/pen was sampled to evaluate the serum metabolic profile. After 28 days, a reduction in diarrhoea incidence was observed in the MIX compared to CTRL group (<i>p</i> < 0.05). In addition, compared to CTRL, MIX showed a higher lactobacilli:coliform ratio and increased <i>Prevotella</i> and <i>Fibrobacter</i> genera presence (<i>p</i> < 0.01). The serum metabolic profile showed a decreased level of low-density lipoproteins in the treated group (<i>p</i> < 0.05). In conclusion, a combination of tannin extract, leonardite and tributyrin could decrease diarrhoea incidence and modulate the gut microbiota. |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T10:39:50Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-3ceb268a09d24df28fcaf4f9e676a16e2023-11-21T23:00:47ZengMDPI AGAnimals2076-26152021-06-01116169310.3390/ani11061693Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned PigletsMatteo Dell’Anno0Serena Reggi1Valentina Caprarulo2Monika Hejna3Carlo Angelo Sgoifo Rossi4Maria Luisa Callegari5Antonella Baldi6Luciana Rossi7Department of Health, Animal Science and Food Safety “Carlo Cantoni” (VESPA), Università Degli Studi di Milano, 26900 Lodi, ItalyDepartment of Health, Animal Science and Food Safety “Carlo Cantoni” (VESPA), Università Degli Studi di Milano, 26900 Lodi, ItalyDepartment of Molecular and Translational Medicine (DMMT), Università Degli Studi di Brescia, 25123 Brescia, ItalyDepartment of Health, Animal Science and Food Safety “Carlo Cantoni” (VESPA), Università Degli Studi di Milano, 26900 Lodi, ItalyDepartment of Health, Animal Science and Food Safety “Carlo Cantoni” (VESPA), Università Degli Studi di Milano, 26900 Lodi, ItalyDepartment for Sustainable Food Process (DiSTAS), Università Cattolica del Sacro Cuore, 29122 Piacenza, ItalyDepartment of Health, Animal Science and Food Safety “Carlo Cantoni” (VESPA), Università Degli Studi di Milano, 26900 Lodi, ItalyDepartment of Health, Animal Science and Food Safety “Carlo Cantoni” (VESPA), Università Degli Studi di Milano, 26900 Lodi, ItalyThe effects of the dietary administration of a combination of Quebracho and Chestnut tannins, leonardite and tributyrin were evaluated in weaned piglets. A total of 168 weaned piglets (Landrace × Large White) were randomly allotted to two experimental groups (6 pens/group, 14 piglets/pen). Animals were fed a basal control diet (CTRL) and a treatment diet (MIX) supplemented with 0.75% tannin extracts, 0.25% leonardite and 0.20% tributyrin for 28 days. Individual body weight and feed intake were recorded weekly. Diarrhoea incidence was recorded by a faecal scoring scale (0–3; considering diarrhoea ≥ 2). At 0 and 28 days, faecal samples were obtained from four piglets/pen for microbiological and chemical analyses of faecal microbiota, which were then assessed by V3-V4 region amplification sequencing. At 28 days, blood from two piglets/pen was sampled to evaluate the serum metabolic profile. After 28 days, a reduction in diarrhoea incidence was observed in the MIX compared to CTRL group (<i>p</i> < 0.05). In addition, compared to CTRL, MIX showed a higher lactobacilli:coliform ratio and increased <i>Prevotella</i> and <i>Fibrobacter</i> genera presence (<i>p</i> < 0.01). The serum metabolic profile showed a decreased level of low-density lipoproteins in the treated group (<i>p</i> < 0.05). In conclusion, a combination of tannin extract, leonardite and tributyrin could decrease diarrhoea incidence and modulate the gut microbiota.https://www.mdpi.com/2076-2615/11/6/1693alternatives to antibioticsantimicrobial resistancefeed additivesfunctional feedtanninsleonardite |
| spellingShingle | Matteo Dell’Anno Serena Reggi Valentina Caprarulo Monika Hejna Carlo Angelo Sgoifo Rossi Maria Luisa Callegari Antonella Baldi Luciana Rossi Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets Animals alternatives to antibiotics antimicrobial resistance feed additives functional feed tannins leonardite |
| title | Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets |
| title_full | Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets |
| title_fullStr | Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets |
| title_full_unstemmed | Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets |
| title_short | Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets |
| title_sort | evaluation of tannin extracts leonardite and tributyrin supplementation on diarrhoea incidence and gut microbiota of weaned piglets |
| topic | alternatives to antibiotics antimicrobial resistance feed additives functional feed tannins leonardite |
| url | https://www.mdpi.com/2076-2615/11/6/1693 |
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