Increased Expression of lncRNA AC000120.7 and SENP3-EIF4A1 in Patients with Acute Respiratory Distress Syndrome Induced by SARS-CoV-2 Infection: A Pilot Study
COVID-19, a disease caused by the SARS-CoV-2 virus, poses significant threats to the respiratory system and other vital organs. Long non-coding RNAs have emerged as influential epigenetic regulators and promising biomarkers in respiratory ailments. The objective of this study was to identify candida...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/9/2342 |
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| author | Javier González-Ramírez Ana Gabriela Leija-Montoya Nicolás Serafín-Higuera Carlos A. Guzmán-Martín Luis M. Amezcua-Guerra Carlos Olvera-Sandoval Jesús René Machado-Contreras Armando Ruiz-Hernández Adrián Hernández-Díazcouder Julia Dolores Estrada-Guzmán Fausto Sánchez-Muñoz |
| author_facet | Javier González-Ramírez Ana Gabriela Leija-Montoya Nicolás Serafín-Higuera Carlos A. Guzmán-Martín Luis M. Amezcua-Guerra Carlos Olvera-Sandoval Jesús René Machado-Contreras Armando Ruiz-Hernández Adrián Hernández-Díazcouder Julia Dolores Estrada-Guzmán Fausto Sánchez-Muñoz |
| author_sort | Javier González-Ramírez |
| collection | DOAJ |
| description | COVID-19, a disease caused by the SARS-CoV-2 virus, poses significant threats to the respiratory system and other vital organs. Long non-coding RNAs have emerged as influential epigenetic regulators and promising biomarkers in respiratory ailments. The objective of this study was to identify candidate lncRNAs in SARS-CoV-2-positive individuals compared to SARS-CoV-2-negative individuals and investigate their potential association with ARDS-CoV-2 (acute respiratory distress syndrome). Employing qRT-PCR, we meticulously examined the expression profiles of a panel comprising 84 inflammation-related lncRNAs in individuals presenting upper respiratory infection symptoms, categorizing them into those testing negative or positive for SARS-CoV-2. Notably, first-phase PSD individuals exhibited significantly elevated levels of AC000120.7 and SENP3-EIF4A1. In addition, we measured the expression of two lncRNAs, AC000120.7 and SENP3-EIF4A1, in patients with ARDS unrelated to SARS-CoV-2 (<i>n</i> = 5) and patients with ARDS induced by SARS-CoV-2 (ARDS-CoV-2, <i>n</i> = 10), and interestingly, expression was also higher among patients with ARDS. Intriguingly, our interaction pathway analysis unveiled potential interactions between lncRNA AC000120.7, various microRNAs, and genes associated with inflammation. This study found higher expression levels of lncRNAs AC000120.7 and SENP3-EIF4A1 in the context of infection-positive COVID-19, particularly within the complex landscape of ARDS. |
| first_indexed | 2024-03-10T22:25:53Z |
| format | Article |
| id | doaj.art-3ceb8b057001401da810a3d337d000d7 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T22:25:53Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3ceb8b057001401da810a3d337d000d72023-11-19T12:04:01ZengMDPI AGMicroorganisms2076-26072023-09-01119234210.3390/microorganisms11092342Increased Expression of lncRNA AC000120.7 and SENP3-EIF4A1 in Patients with Acute Respiratory Distress Syndrome Induced by SARS-CoV-2 Infection: A Pilot StudyJavier González-Ramírez0Ana Gabriela Leija-Montoya1Nicolás Serafín-Higuera2Carlos A. Guzmán-Martín3Luis M. Amezcua-Guerra4Carlos Olvera-Sandoval5Jesús René Machado-Contreras6Armando Ruiz-Hernández7Adrián Hernández-Díazcouder8Julia Dolores Estrada-Guzmán9Fausto Sánchez-Muñoz10Facultad de Enfermería, Universidad Autónoma de Baja California, Av. Álvaro Obregón y Calle “G” S/N, Col. Nueva, Mexicali 21100, Baja California, MexicoFacultad de Medicina Mexicali, Universidad Autónoma de Baja California, Dr. Humberto Torres Sanginés S/N, Centro Cívico, Mexicali 21000, Baja California, MexicoFacultad de Odontología, Universidad Autónoma de Baja California, Zotoluca S/N, Fracc. Calafia, Mexicali 21040, Baja California, MexicoDepartamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, Mexico City 14080, MexicoDepartamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, Mexico City 14080, MexicoFacultad de Medicina Mexicali, Universidad Autónoma de Baja California, Dr. Humberto Torres Sanginés S/N, Centro Cívico, Mexicali 21000, Baja California, MexicoFacultad de Medicina Mexicali, Universidad Autónoma de Baja California, Dr. Humberto Torres Sanginés S/N, Centro Cívico, Mexicali 21000, Baja California, MexicoFacultad de Medicina Mexicali, Universidad Autónoma de Baja California, Dr. Humberto Torres Sanginés S/N, Centro Cívico, Mexicali 21000, Baja California, MexicoDepartamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, Mexico City 14080, MexicoFacultad de Medicina Mexicali, Universidad Autónoma de Baja California, Dr. Humberto Torres Sanginés S/N, Centro Cívico, Mexicali 21000, Baja California, MexicoDepartamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, Mexico City 14080, MexicoCOVID-19, a disease caused by the SARS-CoV-2 virus, poses significant threats to the respiratory system and other vital organs. Long non-coding RNAs have emerged as influential epigenetic regulators and promising biomarkers in respiratory ailments. The objective of this study was to identify candidate lncRNAs in SARS-CoV-2-positive individuals compared to SARS-CoV-2-negative individuals and investigate their potential association with ARDS-CoV-2 (acute respiratory distress syndrome). Employing qRT-PCR, we meticulously examined the expression profiles of a panel comprising 84 inflammation-related lncRNAs in individuals presenting upper respiratory infection symptoms, categorizing them into those testing negative or positive for SARS-CoV-2. Notably, first-phase PSD individuals exhibited significantly elevated levels of AC000120.7 and SENP3-EIF4A1. In addition, we measured the expression of two lncRNAs, AC000120.7 and SENP3-EIF4A1, in patients with ARDS unrelated to SARS-CoV-2 (<i>n</i> = 5) and patients with ARDS induced by SARS-CoV-2 (ARDS-CoV-2, <i>n</i> = 10), and interestingly, expression was also higher among patients with ARDS. Intriguingly, our interaction pathway analysis unveiled potential interactions between lncRNA AC000120.7, various microRNAs, and genes associated with inflammation. This study found higher expression levels of lncRNAs AC000120.7 and SENP3-EIF4A1 in the context of infection-positive COVID-19, particularly within the complex landscape of ARDS.https://www.mdpi.com/2076-2607/11/9/2342COVID-19SARS-CoV-2long non-coding RNAacute respiratory distress syndrome |
| spellingShingle | Javier González-Ramírez Ana Gabriela Leija-Montoya Nicolás Serafín-Higuera Carlos A. Guzmán-Martín Luis M. Amezcua-Guerra Carlos Olvera-Sandoval Jesús René Machado-Contreras Armando Ruiz-Hernández Adrián Hernández-Díazcouder Julia Dolores Estrada-Guzmán Fausto Sánchez-Muñoz Increased Expression of lncRNA AC000120.7 and SENP3-EIF4A1 in Patients with Acute Respiratory Distress Syndrome Induced by SARS-CoV-2 Infection: A Pilot Study Microorganisms COVID-19 SARS-CoV-2 long non-coding RNA acute respiratory distress syndrome |
| title | Increased Expression of lncRNA AC000120.7 and SENP3-EIF4A1 in Patients with Acute Respiratory Distress Syndrome Induced by SARS-CoV-2 Infection: A Pilot Study |
| title_full | Increased Expression of lncRNA AC000120.7 and SENP3-EIF4A1 in Patients with Acute Respiratory Distress Syndrome Induced by SARS-CoV-2 Infection: A Pilot Study |
| title_fullStr | Increased Expression of lncRNA AC000120.7 and SENP3-EIF4A1 in Patients with Acute Respiratory Distress Syndrome Induced by SARS-CoV-2 Infection: A Pilot Study |
| title_full_unstemmed | Increased Expression of lncRNA AC000120.7 and SENP3-EIF4A1 in Patients with Acute Respiratory Distress Syndrome Induced by SARS-CoV-2 Infection: A Pilot Study |
| title_short | Increased Expression of lncRNA AC000120.7 and SENP3-EIF4A1 in Patients with Acute Respiratory Distress Syndrome Induced by SARS-CoV-2 Infection: A Pilot Study |
| title_sort | increased expression of lncrna ac000120 7 and senp3 eif4a1 in patients with acute respiratory distress syndrome induced by sars cov 2 infection a pilot study |
| topic | COVID-19 SARS-CoV-2 long non-coding RNA acute respiratory distress syndrome |
| url | https://www.mdpi.com/2076-2607/11/9/2342 |
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