Multicomponent Analysis of Liuwei Dihuang Pills by a Single Marker Quantification Method and Chemometric Discrimination of Fingerprints
An effective and comprehensive quality evaluation method for Liuwei Dihuang pills (LDP) was established by the simultaneous determination of 8 active components in LDP by the quantitative analysis of multicomponents by single marker (QAMS) method and high-performance liquid chromatography (HPLC) fin...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2023-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2023/6648668 |
| _version_ | 1827082274638659584 |
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| author | Lin Yang Yan Li Yuanfang Hou Yongfu Wu Lihong Tan Zhenqiang Mu Zhaojing Zhu Dan He |
| author_facet | Lin Yang Yan Li Yuanfang Hou Yongfu Wu Lihong Tan Zhenqiang Mu Zhaojing Zhu Dan He |
| author_sort | Lin Yang |
| collection | DOAJ |
| description | An effective and comprehensive quality evaluation method for Liuwei Dihuang pills (LDP) was established by the simultaneous determination of 8 active components in LDP by the quantitative analysis of multicomponents by single marker (QAMS) method and high-performance liquid chromatography (HPLC) fingerprint combined with chemometrics. These 8 active components were determined by QAMS and the external standard method (ESM), and the quantitative results of the two methods were compared to validate the accuracy and feasibility of the QAMS method. 8 active components showed good linear relationships within their ranges, whose average recoveries were 99.7∼102.3%. No significant difference was found (P>0.05) in the quantitative results determined by QAMS and ESM. Furthermore, the fingerprint of LDP was also established, with 11 common peaks identified, and the similarity of the fingerprints of 21 batches of LDP was greater than 0.95. The 21 batches of LDP were basically divided into 3 groups by hierarchical cluster analysis (HCA) and principal component analysis (PCA), and 3 differential markers were screened out by orthogonal partial least squares discriminant analysis (OPLS-DA). The established QAMS method is accurate, economical, fast, and convenient and can simultaneously determine the content of 8 active components in LDP. HPLC fingerprint combined with chemometric analysis more comprehensively evaluated the quality consistency of different batches of LDP and analyzed the markers that cause quality differences between batches. It can provide a scientific basis and reference of quality consistency evaluation for the manufacturers and drug regulatory departments of the preparation. |
| first_indexed | 2024-03-11T22:33:41Z |
| format | Article |
| id | doaj.art-3cedc267551342e79a5d3a4266f9a55a |
| institution | Directory Open Access Journal |
| issn | 2090-8873 |
| language | English |
| last_indexed | 2025-03-20T03:30:39Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj.art-3cedc267551342e79a5d3a4266f9a55a2024-10-03T07:06:34ZengHindawi LimitedJournal of Analytical Methods in Chemistry2090-88732023-01-01202310.1155/2023/6648668Multicomponent Analysis of Liuwei Dihuang Pills by a Single Marker Quantification Method and Chemometric Discrimination of FingerprintsLin Yang0Yan Li1Yuanfang Hou2Yongfu Wu3Lihong Tan4Zhenqiang Mu5Zhaojing Zhu6Dan He7Chongqing Pharmaceutical Preparation Engineering Technology Research CenterChongqing Pharmaceutical Preparation Engineering Technology Research CenterChongqing Pharmaceutical Preparation Engineering Technology Research CenterTraditional Chinese Medicine Factory Co. Ltd.Chongqing Pharmaceutical Preparation Engineering Technology Research CenterChongqing Pharmaceutical Preparation Engineering Technology Research CenterChongqing Pharmaceutical Preparation Engineering Technology Research CenterChongqing Research Center for Pharmaceutical EngineeringAn effective and comprehensive quality evaluation method for Liuwei Dihuang pills (LDP) was established by the simultaneous determination of 8 active components in LDP by the quantitative analysis of multicomponents by single marker (QAMS) method and high-performance liquid chromatography (HPLC) fingerprint combined with chemometrics. These 8 active components were determined by QAMS and the external standard method (ESM), and the quantitative results of the two methods were compared to validate the accuracy and feasibility of the QAMS method. 8 active components showed good linear relationships within their ranges, whose average recoveries were 99.7∼102.3%. No significant difference was found (P>0.05) in the quantitative results determined by QAMS and ESM. Furthermore, the fingerprint of LDP was also established, with 11 common peaks identified, and the similarity of the fingerprints of 21 batches of LDP was greater than 0.95. The 21 batches of LDP were basically divided into 3 groups by hierarchical cluster analysis (HCA) and principal component analysis (PCA), and 3 differential markers were screened out by orthogonal partial least squares discriminant analysis (OPLS-DA). The established QAMS method is accurate, economical, fast, and convenient and can simultaneously determine the content of 8 active components in LDP. HPLC fingerprint combined with chemometric analysis more comprehensively evaluated the quality consistency of different batches of LDP and analyzed the markers that cause quality differences between batches. It can provide a scientific basis and reference of quality consistency evaluation for the manufacturers and drug regulatory departments of the preparation.http://dx.doi.org/10.1155/2023/6648668 |
| spellingShingle | Lin Yang Yan Li Yuanfang Hou Yongfu Wu Lihong Tan Zhenqiang Mu Zhaojing Zhu Dan He Multicomponent Analysis of Liuwei Dihuang Pills by a Single Marker Quantification Method and Chemometric Discrimination of Fingerprints Journal of Analytical Methods in Chemistry |
| title | Multicomponent Analysis of Liuwei Dihuang Pills by a Single Marker Quantification Method and Chemometric Discrimination of Fingerprints |
| title_full | Multicomponent Analysis of Liuwei Dihuang Pills by a Single Marker Quantification Method and Chemometric Discrimination of Fingerprints |
| title_fullStr | Multicomponent Analysis of Liuwei Dihuang Pills by a Single Marker Quantification Method and Chemometric Discrimination of Fingerprints |
| title_full_unstemmed | Multicomponent Analysis of Liuwei Dihuang Pills by a Single Marker Quantification Method and Chemometric Discrimination of Fingerprints |
| title_short | Multicomponent Analysis of Liuwei Dihuang Pills by a Single Marker Quantification Method and Chemometric Discrimination of Fingerprints |
| title_sort | multicomponent analysis of liuwei dihuang pills by a single marker quantification method and chemometric discrimination of fingerprints |
| url | http://dx.doi.org/10.1155/2023/6648668 |
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