Transcriptional dynamics and regulatory function of milRNAs in Ascosphaera apis invading Apis mellifera larvae
In the present study, small RNA (sRNA) data from Ascosphaera apis were filtered from sRNA-seq datasets from the gut tissues of A. apis-infected Apis mellifera ligustica worker larvae, which were combined with the previously gained sRNA-seq data from A. apis spores to screen differentially expressed...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-04-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1355035/full |
| _version_ | 1797219307467309056 |
|---|---|
| author | Xiaoxue Fan Xuze Gao He Zang Zhitan Liu Xin Jing Xiaoyu Liu Sijia Guo Haibin Jiang Ying Wu Zhijian Huang Dafu Chen Dafu Chen Dafu Chen Rui Guo Rui Guo Rui Guo |
| author_facet | Xiaoxue Fan Xuze Gao He Zang Zhitan Liu Xin Jing Xiaoyu Liu Sijia Guo Haibin Jiang Ying Wu Zhijian Huang Dafu Chen Dafu Chen Dafu Chen Rui Guo Rui Guo Rui Guo |
| author_sort | Xiaoxue Fan |
| collection | DOAJ |
| description | In the present study, small RNA (sRNA) data from Ascosphaera apis were filtered from sRNA-seq datasets from the gut tissues of A. apis-infected Apis mellifera ligustica worker larvae, which were combined with the previously gained sRNA-seq data from A. apis spores to screen differentially expressed milRNAs (DEmilRNAs), followed by trend analysis and investigation of the DEmilRNAs in relation to significant trends. Additionally, the interactions between the DEmilRNAs and their target mRNAs were verified using a dual-luciferase reporter assay. In total, 974 A. apis milRNAs were identified. The first base of these milRNAs was biased toward U. The expression of six milRNAs was confirmed by stem–loop RT-PCR, and the sequences of milR-3245-y and milR-10285-y were validated using Sanger sequencing. These miRNAs grouped into four significant trends, with the target mRNAs of DEmilRNAs involving 42 GO terms and 120 KEGG pathways, such as the fungal-type cell wall and biosynthesis of secondary metabolites. Further investigation demonstrated that 299 DEmilRNAs (novel-m0011-3p, milR-10048-y, bantam-y, etc.) potentially targeted nine genes encoding secondary metabolite-associated enzymes, while 258 (milR-25-y, milR-14-y, milR-932-x, etc.) and 419 (milR-4561-y, milR-10125-y, let-7-x, etc.) DEmilRNAs putatively targeted virulence factor-encoded genes and nine genes involved in the MAPK signaling pathway, respectively. Additionally, the interaction between ADM-B and milR-6882-x, as well as between PKIA and milR-7009-x were verified. Together, these results not only offer a basis for clarifying the mechanisms underlying DEmilRNA-regulated pathogenesis of A. apis and a novel insight into the interaction between A. apis and honey bee larvae, but also provide candidate DEmilRNA–gene axis for further investigation. |
| first_indexed | 2024-04-24T12:31:34Z |
| format | Article |
| id | doaj.art-3cef2cde5625470982a9fb7a9242f021 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-24T12:31:34Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-3cef2cde5625470982a9fb7a9242f0212024-04-08T04:23:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-04-011510.3389/fmicb.2024.13550351355035Transcriptional dynamics and regulatory function of milRNAs in Ascosphaera apis invading Apis mellifera larvaeXiaoxue Fan0Xuze Gao1He Zang2Zhitan Liu3Xin Jing4Xiaoyu Liu5Sijia Guo6Haibin Jiang7Ying Wu8Zhijian Huang9Dafu Chen10Dafu Chen11Dafu Chen12Rui Guo13Rui Guo14Rui Guo15College of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou, ChinaJilin Apicultural Research Institute, Jilin, ChinaJilin Apicultural Research Institute, Jilin, ChinaAnimal Husbandry Terminus of Sichuan Provincial Department of Agriculture and Rural Affairs, Chengdu, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou, ChinaNational and Local United Engineering Laboratory of Natural Biotoxin, Fuzhou, ChinaApitherapy Research Institute of Fujian Province, Fuzhou, ChinaCollege of Bee Science and Biomedicine, Fujian Agriculture and Forestry University, Fuzhou, ChinaNational and Local United Engineering Laboratory of Natural Biotoxin, Fuzhou, ChinaApitherapy Research Institute of Fujian Province, Fuzhou, ChinaIn the present study, small RNA (sRNA) data from Ascosphaera apis were filtered from sRNA-seq datasets from the gut tissues of A. apis-infected Apis mellifera ligustica worker larvae, which were combined with the previously gained sRNA-seq data from A. apis spores to screen differentially expressed milRNAs (DEmilRNAs), followed by trend analysis and investigation of the DEmilRNAs in relation to significant trends. Additionally, the interactions between the DEmilRNAs and their target mRNAs were verified using a dual-luciferase reporter assay. In total, 974 A. apis milRNAs were identified. The first base of these milRNAs was biased toward U. The expression of six milRNAs was confirmed by stem–loop RT-PCR, and the sequences of milR-3245-y and milR-10285-y were validated using Sanger sequencing. These miRNAs grouped into four significant trends, with the target mRNAs of DEmilRNAs involving 42 GO terms and 120 KEGG pathways, such as the fungal-type cell wall and biosynthesis of secondary metabolites. Further investigation demonstrated that 299 DEmilRNAs (novel-m0011-3p, milR-10048-y, bantam-y, etc.) potentially targeted nine genes encoding secondary metabolite-associated enzymes, while 258 (milR-25-y, milR-14-y, milR-932-x, etc.) and 419 (milR-4561-y, milR-10125-y, let-7-x, etc.) DEmilRNAs putatively targeted virulence factor-encoded genes and nine genes involved in the MAPK signaling pathway, respectively. Additionally, the interaction between ADM-B and milR-6882-x, as well as between PKIA and milR-7009-x were verified. Together, these results not only offer a basis for clarifying the mechanisms underlying DEmilRNA-regulated pathogenesis of A. apis and a novel insight into the interaction between A. apis and honey bee larvae, but also provide candidate DEmilRNA–gene axis for further investigation.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1355035/fullAscosphaera apischalkbroodApis melliferalarvamilRNAregulation |
| spellingShingle | Xiaoxue Fan Xuze Gao He Zang Zhitan Liu Xin Jing Xiaoyu Liu Sijia Guo Haibin Jiang Ying Wu Zhijian Huang Dafu Chen Dafu Chen Dafu Chen Rui Guo Rui Guo Rui Guo Transcriptional dynamics and regulatory function of milRNAs in Ascosphaera apis invading Apis mellifera larvae Frontiers in Microbiology Ascosphaera apis chalkbrood Apis mellifera larva milRNA regulation |
| title | Transcriptional dynamics and regulatory function of milRNAs in Ascosphaera apis invading Apis mellifera larvae |
| title_full | Transcriptional dynamics and regulatory function of milRNAs in Ascosphaera apis invading Apis mellifera larvae |
| title_fullStr | Transcriptional dynamics and regulatory function of milRNAs in Ascosphaera apis invading Apis mellifera larvae |
| title_full_unstemmed | Transcriptional dynamics and regulatory function of milRNAs in Ascosphaera apis invading Apis mellifera larvae |
| title_short | Transcriptional dynamics and regulatory function of milRNAs in Ascosphaera apis invading Apis mellifera larvae |
| title_sort | transcriptional dynamics and regulatory function of milrnas in ascosphaera apis invading apis mellifera larvae |
| topic | Ascosphaera apis chalkbrood Apis mellifera larva milRNA regulation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1355035/full |
| work_keys_str_mv | AT xiaoxuefan transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT xuzegao transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT hezang transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT zhitanliu transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT xinjing transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT xiaoyuliu transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT sijiaguo transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT haibinjiang transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT yingwu transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT zhijianhuang transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT dafuchen transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT dafuchen transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT dafuchen transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT ruiguo transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT ruiguo transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae AT ruiguo transcriptionaldynamicsandregulatoryfunctionofmilrnasinascosphaeraapisinvadingapismelliferalarvae |