SEPARATION OF CELL POPULATIONS BY SUPER-PARAMAGNETIC PARTICLES WITH CONTROLLED SURFACE FUNCTIONALITY
The recognition and isolation of specific mammalian cells by the biocompatible polymer coated super-paramagnetic particles with determined surface functionality were studied. The method of synthesis of nanoscaled particles on a core of iron III oxide (Fe2O3, magemit) coated with a polymer shell cont...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2014-02-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/no1_2014/lutsik.pdf |
| _version_ | 1827837455622995968 |
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| author | Lootsik M. D. Boiko N. M. Mitina N. E. Klyuchivska O. Yu. Lutsyk M. M. Konstantinova T. E. Zaichenko A. S. Stoika R. S. |
| author_facet | Lootsik M. D. Boiko N. M. Mitina N. E. Klyuchivska O. Yu. Lutsyk M. M. Konstantinova T. E. Zaichenko A. S. Stoika R. S. |
| author_sort | Lootsik M. D. |
| collection | DOAJ |
| description | The recognition and isolation of specific mammalian cells by the biocompatible polymer coated super-paramagnetic particles with determined surface functionality were studied. The method of synthesis of nanoscaled particles on a core of iron III oxide (Fe2O3, magemit) coated with a polymer shell containing reactive oligoperoxide groups for attachment of ligands is described.
By using the developed superparamagnetic particles functionalized with peanut agglutinin (PNA) we have separated the sub-populations of PNA+ and PNA– cells from ascites of murine Nemeth-Kellner lymphoma.
In another type of experiment, the particles were opsonized with proteins of the fetal calf serum that improved biocompatibility of the particles and their ingestion by cultivated murine macrophages J774.2. Macrophages loaded with the particles were effeciently separated from the particles free cells by using the magnet. Thus, the developed surface functionalized superparamagnetic particles showed to be a versatile tool for cell separation independent on the mode of particles’ binding with cell surface or their engulfment by the targeted cells. |
| first_indexed | 2024-03-12T06:44:03Z |
| format | Article |
| id | doaj.art-3cf032b5a5ab4f8eb4d5de874112d132 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T06:44:03Z |
| publishDate | 2014-02-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-3cf032b5a5ab4f8eb4d5de874112d1322023-09-03T00:44:42ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2014-02-0171808610.15407/biotech7.01.080SEPARATION OF CELL POPULATIONS BY SUPER-PARAMAGNETIC PARTICLES WITH CONTROLLED SURFACE FUNCTIONALITYLootsik M. D.0Boiko N. M.1Mitina N. E.2Klyuchivska O. Yu.3Lutsyk M. M.4 Konstantinova T. E.5Zaichenko A. S.6Stoika R. S.7Institute of Cell Biology of National Academy of Sciences of Ukraine, LvivInstitute of Cell Biology of National Academy of Sciences of Ukraine, LvivLviv National Polytechnic University, UkraineInstitute of Cell Biology of National Academy of Sciences of Ukraine, LvivDanylo Halytsky National Medical University, Lviv, UkraineGalkin Institute of Physics and Engineering of National Academy of Sciences of Ukraine, DonetskLviv National Polytechnic University, UkraineInstitute of Cell Biology of National Academy of Sciences of Ukraine, LvivThe recognition and isolation of specific mammalian cells by the biocompatible polymer coated super-paramagnetic particles with determined surface functionality were studied. The method of synthesis of nanoscaled particles on a core of iron III oxide (Fe2O3, magemit) coated with a polymer shell containing reactive oligoperoxide groups for attachment of ligands is described. By using the developed superparamagnetic particles functionalized with peanut agglutinin (PNA) we have separated the sub-populations of PNA+ and PNA– cells from ascites of murine Nemeth-Kellner lymphoma. In another type of experiment, the particles were opsonized with proteins of the fetal calf serum that improved biocompatibility of the particles and their ingestion by cultivated murine macrophages J774.2. Macrophages loaded with the particles were effeciently separated from the particles free cells by using the magnet. Thus, the developed surface functionalized superparamagnetic particles showed to be a versatile tool for cell separation independent on the mode of particles’ binding with cell surface or their engulfment by the targeted cells.http://biotechnology.kiev.ua/images/storage/no1_2014/lutsik.pdfsuper-paramagnetic particlescell selectionNK/Ly lymphomamacrophages J774.2. |
| spellingShingle | Lootsik M. D. Boiko N. M. Mitina N. E. Klyuchivska O. Yu. Lutsyk M. M. Konstantinova T. E. Zaichenko A. S. Stoika R. S. SEPARATION OF CELL POPULATIONS BY SUPER-PARAMAGNETIC PARTICLES WITH CONTROLLED SURFACE FUNCTIONALITY Biotechnologia Acta super-paramagnetic particles cell selection NK/Ly lymphoma macrophages J774.2. |
| title | SEPARATION OF CELL POPULATIONS BY SUPER-PARAMAGNETIC PARTICLES WITH CONTROLLED SURFACE FUNCTIONALITY |
| title_full | SEPARATION OF CELL POPULATIONS BY SUPER-PARAMAGNETIC PARTICLES WITH CONTROLLED SURFACE FUNCTIONALITY |
| title_fullStr | SEPARATION OF CELL POPULATIONS BY SUPER-PARAMAGNETIC PARTICLES WITH CONTROLLED SURFACE FUNCTIONALITY |
| title_full_unstemmed | SEPARATION OF CELL POPULATIONS BY SUPER-PARAMAGNETIC PARTICLES WITH CONTROLLED SURFACE FUNCTIONALITY |
| title_short | SEPARATION OF CELL POPULATIONS BY SUPER-PARAMAGNETIC PARTICLES WITH CONTROLLED SURFACE FUNCTIONALITY |
| title_sort | separation of cell populations by super paramagnetic particles with controlled surface functionality |
| topic | super-paramagnetic particles cell selection NK/Ly lymphoma macrophages J774.2. |
| url | http://biotechnology.kiev.ua/images/storage/no1_2014/lutsik.pdf |
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