Phylogenetic analysis and biocontrol potential of entomopathogenic fungi against Tropinota (=Epicometis) hirta (Poda) (Coleoptera: Cetoniidae) and the apple scab disease Ventura inaequalis
Abstract Background Entomopathogenic fungi have long been used as a biopeptide in the biological control of insect pests in agriculture, forestry and veterinary. At the same time, it is known that these fungi have positive effects on plant growth, health, and the antagonist effect against various pl...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-01-01
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| Series: | Egyptian Journal of Biological Pest Control |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41938-023-00652-9 |
| _version_ | 1811175677996564480 |
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| author | Alperen Kutalmış Zeynep Terzioğlu Rabia Hande Şen Ali Sevim |
| author_facet | Alperen Kutalmış Zeynep Terzioğlu Rabia Hande Şen Ali Sevim |
| author_sort | Alperen Kutalmış |
| collection | DOAJ |
| description | Abstract Background Entomopathogenic fungi have long been used as a biopeptide in the biological control of insect pests in agriculture, forestry and veterinary. At the same time, it is known that these fungi have positive effects on plant growth, health, and the antagonist effect against various plant pathogens. Entomopathogenic fungal isolation was performed from soil samples collected from apple orchards and Tropinota (=Epicometis) hirta (Poda) (Coleoptera: Cetoniidae) adults which is an important pest of fruit trees. The isolated fungi were identified at the species level by phylogenetic analysis based on multi-locus sequence approach using various gene sequences (ITS, rpb1, bloc, EF1-α and β-tubulin). The fungi obtained were tested against T. hirta and the apple scab disease, Ventura inaequalis under laboratory conditions. Results Three (Bz isolates) and 15 (AK isolates) entomopathogenic fungi (EPF) were isolated from T. hirta adults and 48 soil samples, respectively. The isolated fungi were identified as Beauveria bassiana (Bz-1, Bz-2, AK-10, AK-14, AK-17, and AK-18), Metarhizium robertsii (Bz-3, AK-4, AK-5, AK-6, AK-7, AK-8, AK-9, AK-11, AK-13, AK-15, and AK-16) and Metarhizium sp. (AK-12). All isolated fungi were tested against T. hirta adults, causing the same mortality but different mycosis values. In addition, the antagonistic effects of fungal isolates against V. inaequalis, the important apple pathogen, were determined and the highest effect was obtained from B. bassiana AK-10 with 69.3%. Conclusion This is the first study to determine the effectiveness of EPF against T. hirta and V. inaequalis, and the results obtained are thought to be useful for the biological control of both pests. |
| first_indexed | 2024-04-10T19:40:43Z |
| format | Article |
| id | doaj.art-3cf09ec82b0e4135afc163965cc03b01 |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-04-10T19:40:43Z |
| publishDate | 2023-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-3cf09ec82b0e4135afc163965cc03b012023-01-29T12:21:38ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422023-01-0133111210.1186/s41938-023-00652-9Phylogenetic analysis and biocontrol potential of entomopathogenic fungi against Tropinota (=Epicometis) hirta (Poda) (Coleoptera: Cetoniidae) and the apple scab disease Ventura inaequalisAlperen Kutalmış0Zeynep Terzioğlu1Rabia Hande Şen2Ali Sevim3Department of Plant Protection, Faculty of Agriculture, Kırşehir Ahi Evran UniversityDepartment of Plant Protection, Faculty of Agriculture, Kırşehir Ahi Evran UniversityDepartment of Plant Protection, Faculty of Agriculture, Kırşehir Ahi Evran UniversityDepartment of Plant Protection, Faculty of Agriculture, Kırşehir Ahi Evran UniversityAbstract Background Entomopathogenic fungi have long been used as a biopeptide in the biological control of insect pests in agriculture, forestry and veterinary. At the same time, it is known that these fungi have positive effects on plant growth, health, and the antagonist effect against various plant pathogens. Entomopathogenic fungal isolation was performed from soil samples collected from apple orchards and Tropinota (=Epicometis) hirta (Poda) (Coleoptera: Cetoniidae) adults which is an important pest of fruit trees. The isolated fungi were identified at the species level by phylogenetic analysis based on multi-locus sequence approach using various gene sequences (ITS, rpb1, bloc, EF1-α and β-tubulin). The fungi obtained were tested against T. hirta and the apple scab disease, Ventura inaequalis under laboratory conditions. Results Three (Bz isolates) and 15 (AK isolates) entomopathogenic fungi (EPF) were isolated from T. hirta adults and 48 soil samples, respectively. The isolated fungi were identified as Beauveria bassiana (Bz-1, Bz-2, AK-10, AK-14, AK-17, and AK-18), Metarhizium robertsii (Bz-3, AK-4, AK-5, AK-6, AK-7, AK-8, AK-9, AK-11, AK-13, AK-15, and AK-16) and Metarhizium sp. (AK-12). All isolated fungi were tested against T. hirta adults, causing the same mortality but different mycosis values. In addition, the antagonistic effects of fungal isolates against V. inaequalis, the important apple pathogen, were determined and the highest effect was obtained from B. bassiana AK-10 with 69.3%. Conclusion This is the first study to determine the effectiveness of EPF against T. hirta and V. inaequalis, and the results obtained are thought to be useful for the biological control of both pests.https://doi.org/10.1186/s41938-023-00652-9Tropinota (=Epicometis) hirtaVentura inaequalisBeauveriaMetarhiziumBiological control |
| spellingShingle | Alperen Kutalmış Zeynep Terzioğlu Rabia Hande Şen Ali Sevim Phylogenetic analysis and biocontrol potential of entomopathogenic fungi against Tropinota (=Epicometis) hirta (Poda) (Coleoptera: Cetoniidae) and the apple scab disease Ventura inaequalis Egyptian Journal of Biological Pest Control Tropinota (=Epicometis) hirta Ventura inaequalis Beauveria Metarhizium Biological control |
| title | Phylogenetic analysis and biocontrol potential of entomopathogenic fungi against Tropinota (=Epicometis) hirta (Poda) (Coleoptera: Cetoniidae) and the apple scab disease Ventura inaequalis |
| title_full | Phylogenetic analysis and biocontrol potential of entomopathogenic fungi against Tropinota (=Epicometis) hirta (Poda) (Coleoptera: Cetoniidae) and the apple scab disease Ventura inaequalis |
| title_fullStr | Phylogenetic analysis and biocontrol potential of entomopathogenic fungi against Tropinota (=Epicometis) hirta (Poda) (Coleoptera: Cetoniidae) and the apple scab disease Ventura inaequalis |
| title_full_unstemmed | Phylogenetic analysis and biocontrol potential of entomopathogenic fungi against Tropinota (=Epicometis) hirta (Poda) (Coleoptera: Cetoniidae) and the apple scab disease Ventura inaequalis |
| title_short | Phylogenetic analysis and biocontrol potential of entomopathogenic fungi against Tropinota (=Epicometis) hirta (Poda) (Coleoptera: Cetoniidae) and the apple scab disease Ventura inaequalis |
| title_sort | phylogenetic analysis and biocontrol potential of entomopathogenic fungi against tropinota epicometis hirta poda coleoptera cetoniidae and the apple scab disease ventura inaequalis |
| topic | Tropinota (=Epicometis) hirta Ventura inaequalis Beauveria Metarhizium Biological control |
| url | https://doi.org/10.1186/s41938-023-00652-9 |
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