The Efficacy of the Period of Saccharification on Oil Palm (Elaeis guineensis) Trunk Sap Hydrolysis
This study investigates the enzymatic hydrolysis rate of Oil Palm (Elaeis guineensis) Trunk (OPT) sap in terms of the length of saccharification process with the aim to elevate sugar production. Emphasis was placed on the reaction time and addition of supplements such epsom salt (MgSO4) and alanine...
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2018-07-01
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| Series: | International Journal of Technology |
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| Online Access: | http://ijtech.eng.ui.ac.id/article/view/1808 |
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| author | Nazia Hossain Juliana Zaini Rafidah Jalil T.M.I. Mahlia |
| author_facet | Nazia Hossain Juliana Zaini Rafidah Jalil T.M.I. Mahlia |
| author_sort | Nazia Hossain |
| collection | DOAJ |
| description | This study investigates the enzymatic hydrolysis rate of Oil Palm (Elaeis guineensis) Trunk (OPT) sap in terms of the length of saccharification process with the aim to elevate sugar production. Emphasis was placed on the reaction time and addition of supplements such epsom salt (MgSO4) and alanine amino acid (C3H7NO2) to accelerate the efficiency of Saccharomyces cerevisiae containing the enzyme invertase. A whole oil palm trunk was divided into four different sections, upper, middle-1, middle-2 and bottom with separate experiments over 10 days enzymatic reaction period. The highest saccharification rate was shown as 13.47% on the tenth day. This result indicates that the increase in the saccharification rate was positively correlated with the length of hydrolysis. Moreover, the sample with nutrients achieved the highest sugar output, 17.91% on the fourth day of hydrolysis which was 4.44% higher than the hydrolysis rate of the sample without nutrients. In the presence of complex OPT sugars, together with other essential elements, epsom salt and alanine amino acid, S.cerevisiae achieved a higher hydrolysis metabolism to simple sugars as the cells strived to produce energy and regenerated the invertase. Moreover, the upper part of the OPT rendered the highest potential for sugar production with levels of 21.2% with supplements and 15.6% without. From this experimental analysis, a conventional saccharification method was optimized through the addition of nutrients and a prolonged (10 days) hydrolysis process which yielded an increase in sugar production. |
| first_indexed | 2024-04-11T02:09:23Z |
| format | Article |
| id | doaj.art-3cf26b9898074242b7bc80f9a123ac57 |
| institution | Directory Open Access Journal |
| issn | 2086-9614 2087-2100 |
| language | English |
| last_indexed | 2024-04-11T02:09:23Z |
| publishDate | 2018-07-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | International Journal of Technology |
| spelling | doaj.art-3cf26b9898074242b7bc80f9a123ac572023-01-03T02:27:05ZengUniversitas IndonesiaInternational Journal of Technology2086-96142087-21002018-07-019465266210.14716/ijtech.v9i4.18081808The Efficacy of the Period of Saccharification on Oil Palm (Elaeis guineensis) Trunk Sap HydrolysisNazia Hossain0Juliana Zaini1Rafidah Jalil2T.M.I. Mahlia3Department of Chemical and Process Engineering, Faculty of Integrated Technologies, Universiti Brunei Darussalam, Bandar Seri Begawan, Brunei Darussalam.Universiti Brunei DarussalamForest Research Institute MalaysiaUniversiti Tenaga NationalThis study investigates the enzymatic hydrolysis rate of Oil Palm (Elaeis guineensis) Trunk (OPT) sap in terms of the length of saccharification process with the aim to elevate sugar production. Emphasis was placed on the reaction time and addition of supplements such epsom salt (MgSO4) and alanine amino acid (C3H7NO2) to accelerate the efficiency of Saccharomyces cerevisiae containing the enzyme invertase. A whole oil palm trunk was divided into four different sections, upper, middle-1, middle-2 and bottom with separate experiments over 10 days enzymatic reaction period. The highest saccharification rate was shown as 13.47% on the tenth day. This result indicates that the increase in the saccharification rate was positively correlated with the length of hydrolysis. Moreover, the sample with nutrients achieved the highest sugar output, 17.91% on the fourth day of hydrolysis which was 4.44% higher than the hydrolysis rate of the sample without nutrients. In the presence of complex OPT sugars, together with other essential elements, epsom salt and alanine amino acid, S.cerevisiae achieved a higher hydrolysis metabolism to simple sugars as the cells strived to produce energy and regenerated the invertase. Moreover, the upper part of the OPT rendered the highest potential for sugar production with levels of 21.2% with supplements and 15.6% without. From this experimental analysis, a conventional saccharification method was optimized through the addition of nutrients and a prolonged (10 days) hydrolysis process which yielded an increase in sugar production.http://ijtech.eng.ui.ac.id/article/view/1808HydrolysisHydrolysis timeOil palm trunkSaccharificationSaccharomyces cerevisiaeSugar conversion |
| spellingShingle | Nazia Hossain Juliana Zaini Rafidah Jalil T.M.I. Mahlia The Efficacy of the Period of Saccharification on Oil Palm (Elaeis guineensis) Trunk Sap Hydrolysis International Journal of Technology Hydrolysis Hydrolysis time Oil palm trunk Saccharification Saccharomyces cerevisiae Sugar conversion |
| title | The Efficacy of the Period of Saccharification on Oil Palm (Elaeis guineensis) Trunk Sap Hydrolysis |
| title_full | The Efficacy of the Period of Saccharification on Oil Palm (Elaeis guineensis) Trunk Sap Hydrolysis |
| title_fullStr | The Efficacy of the Period of Saccharification on Oil Palm (Elaeis guineensis) Trunk Sap Hydrolysis |
| title_full_unstemmed | The Efficacy of the Period of Saccharification on Oil Palm (Elaeis guineensis) Trunk Sap Hydrolysis |
| title_short | The Efficacy of the Period of Saccharification on Oil Palm (Elaeis guineensis) Trunk Sap Hydrolysis |
| title_sort | efficacy of the period of saccharification on oil palm elaeis guineensis trunk sap hydrolysis |
| topic | Hydrolysis Hydrolysis time Oil palm trunk Saccharification Saccharomyces cerevisiae Sugar conversion |
| url | http://ijtech.eng.ui.ac.id/article/view/1808 |
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