Prediction by promoter logic in bacterial quorum sensing.
Quorum-sensing systems mediate chemical communication between bacterial cells, coordinating cell-density-dependent processes like biofilm formation and virulence-factor expression. In the proteobacterial LuxI/LuxR quorum sensing paradigm, a signaling molecule generated by an enzyme (LuxI) diffuses b...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS Computational Biology |
| Online Access: | http://europepmc.org/articles/PMC3261908?pdf=render |
| _version_ | 1818435982352973824 |
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| author | Navneet Rai Rajat Anand Krishna Ramkumar Varun Sreenivasan Sugat Dabholkar K V Venkatesh Mukund Thattai |
| author_facet | Navneet Rai Rajat Anand Krishna Ramkumar Varun Sreenivasan Sugat Dabholkar K V Venkatesh Mukund Thattai |
| author_sort | Navneet Rai |
| collection | DOAJ |
| description | Quorum-sensing systems mediate chemical communication between bacterial cells, coordinating cell-density-dependent processes like biofilm formation and virulence-factor expression. In the proteobacterial LuxI/LuxR quorum sensing paradigm, a signaling molecule generated by an enzyme (LuxI) diffuses between cells and allosterically stimulates a transcriptional regulator (LuxR) to activate its cognate promoter (pR). By expressing either LuxI or LuxR in positive feedback from pR, these versatile systems can generate smooth (monostable) or abrupt (bistable) density-dependent responses to suit the ecological context. Here we combine theory and experiment to demonstrate that the promoter logic of pR - its measured activity as a function of LuxI and LuxR levels - contains all the biochemical information required to quantitatively predict the responses of such feedback loops. The interplay of promoter logic with feedback topology underlies the versatility of the LuxI/LuxR paradigm: LuxR and LuxI positive-feedback systems show dramatically different responses, while a dual positive/negative-feedback system displays synchronized oscillations. These results highlight the dual utility of promoter logic: to probe microscopic parameters and predict macroscopic phenotype. |
| first_indexed | 2024-12-14T17:01:32Z |
| format | Article |
| id | doaj.art-3cf283dda0604e42a445f2e72df731c3 |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-12-14T17:01:32Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-3cf283dda0604e42a445f2e72df731c32022-12-21T22:53:49ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582012-01-0181e100236110.1371/journal.pcbi.1002361Prediction by promoter logic in bacterial quorum sensing.Navneet RaiRajat AnandKrishna RamkumarVarun SreenivasanSugat DabholkarK V VenkateshMukund ThattaiQuorum-sensing systems mediate chemical communication between bacterial cells, coordinating cell-density-dependent processes like biofilm formation and virulence-factor expression. In the proteobacterial LuxI/LuxR quorum sensing paradigm, a signaling molecule generated by an enzyme (LuxI) diffuses between cells and allosterically stimulates a transcriptional regulator (LuxR) to activate its cognate promoter (pR). By expressing either LuxI or LuxR in positive feedback from pR, these versatile systems can generate smooth (monostable) or abrupt (bistable) density-dependent responses to suit the ecological context. Here we combine theory and experiment to demonstrate that the promoter logic of pR - its measured activity as a function of LuxI and LuxR levels - contains all the biochemical information required to quantitatively predict the responses of such feedback loops. The interplay of promoter logic with feedback topology underlies the versatility of the LuxI/LuxR paradigm: LuxR and LuxI positive-feedback systems show dramatically different responses, while a dual positive/negative-feedback system displays synchronized oscillations. These results highlight the dual utility of promoter logic: to probe microscopic parameters and predict macroscopic phenotype.http://europepmc.org/articles/PMC3261908?pdf=render |
| spellingShingle | Navneet Rai Rajat Anand Krishna Ramkumar Varun Sreenivasan Sugat Dabholkar K V Venkatesh Mukund Thattai Prediction by promoter logic in bacterial quorum sensing. PLoS Computational Biology |
| title | Prediction by promoter logic in bacterial quorum sensing. |
| title_full | Prediction by promoter logic in bacterial quorum sensing. |
| title_fullStr | Prediction by promoter logic in bacterial quorum sensing. |
| title_full_unstemmed | Prediction by promoter logic in bacterial quorum sensing. |
| title_short | Prediction by promoter logic in bacterial quorum sensing. |
| title_sort | prediction by promoter logic in bacterial quorum sensing |
| url | http://europepmc.org/articles/PMC3261908?pdf=render |
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