Characterization of avain metapneumovirus subgroup C isolated from chickens in Beijing, China
Avian metapneumovirus (aMPV) is an important causative agent that causes acute respiratory disease and egg-dropping in chickens and turkeys. Here, we characterized an aMPV subgroup C (aMPV/C) from 320-day-old broiler breeder chickens with severe respiratory diseases in Beijing, China, as evidenced b...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122005466 |
| _version_ | 1811210025146777600 |
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| author | Jing Wang Lei Hou Li Wei Xv Yan Shanshan Zhu Rong Quan Zixuan Li Dan Wang Haijun Jiang Jiangwei Song Yongqiu Cui Jue Liu |
| author_facet | Jing Wang Lei Hou Li Wei Xv Yan Shanshan Zhu Rong Quan Zixuan Li Dan Wang Haijun Jiang Jiangwei Song Yongqiu Cui Jue Liu |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | Avian metapneumovirus (aMPV) is an important causative agent that causes acute respiratory disease and egg-dropping in chickens and turkeys. Here, we characterized an aMPV subgroup C (aMPV/C) from 320-day-old broiler breeder chickens with severe respiratory diseases in Beijing, China, as evidenced by RT-PCR typing and confirmation of the nucleoprotein (N) gene sequence. The N gene sequence of the aMPV/C strain (designated BJ17) exhibited no deletions or insertions and possessed 94.6% to 99.6% identity to those of published aMPV/C isolates. The phylogenetic tree of the nucleotide sequences constructed using the neighbor-joining clustering method showed that the BJ17 strain formed one cluster with other aMPV/C viruses and formed one subcluster with published Chinese aMPV/C isolates regardless of Muscovy duck or chicken origins. Comparative analysis of the N proteins showed that a unique amino acid residue D at position 110 might be associated with regional distribution due to its occurrence in all the Chinese aMPV/C isolates only. Strain BJ17 was successfully isolated by cultured Vero cell passage and further inoculated in 3-wk-old specific-pathogen-free chickens for the examination of pathogenicity. Animal experimental results showed that BJ17-inoculated chickens had severe respiratory diseases and inflammatory lesions, as demonstrated by pathological changes and aMPV antigen in the nasal turbinate, tracheae, and lung tissues. These results enrich the available information regarding the epidemiology and pathogenicity of aMPV/C in chickens, which may facilitate the development of effective measures against aMPV/C infection in China. |
| first_indexed | 2024-04-12T04:48:48Z |
| format | Article |
| id | doaj.art-3cf28be1595d4c72bf88dee214ba430f |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-12T04:48:48Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-3cf28be1595d4c72bf88dee214ba430f2022-12-22T03:47:22ZengElsevierPoultry Science0032-57912023-01-011021102250Characterization of avain metapneumovirus subgroup C isolated from chickens in Beijing, ChinaJing Wang0Lei Hou1Li Wei2Xv Yan3Shanshan Zhu4Rong Quan5Zixuan Li6Dan Wang7Haijun Jiang8Jiangwei Song9Yongqiu Cui10Jue Liu11Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Haidian District, Beijing, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Haidian District, Beijing, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Haidian District, Beijing, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Haidian District, Beijing, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Haidian District, Beijing, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Haidian District, Beijing, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Haidian District, Beijing, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Haidian District, Beijing, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Haidian District, Beijing, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China; Corresponding author:Avian metapneumovirus (aMPV) is an important causative agent that causes acute respiratory disease and egg-dropping in chickens and turkeys. Here, we characterized an aMPV subgroup C (aMPV/C) from 320-day-old broiler breeder chickens with severe respiratory diseases in Beijing, China, as evidenced by RT-PCR typing and confirmation of the nucleoprotein (N) gene sequence. The N gene sequence of the aMPV/C strain (designated BJ17) exhibited no deletions or insertions and possessed 94.6% to 99.6% identity to those of published aMPV/C isolates. The phylogenetic tree of the nucleotide sequences constructed using the neighbor-joining clustering method showed that the BJ17 strain formed one cluster with other aMPV/C viruses and formed one subcluster with published Chinese aMPV/C isolates regardless of Muscovy duck or chicken origins. Comparative analysis of the N proteins showed that a unique amino acid residue D at position 110 might be associated with regional distribution due to its occurrence in all the Chinese aMPV/C isolates only. Strain BJ17 was successfully isolated by cultured Vero cell passage and further inoculated in 3-wk-old specific-pathogen-free chickens for the examination of pathogenicity. Animal experimental results showed that BJ17-inoculated chickens had severe respiratory diseases and inflammatory lesions, as demonstrated by pathological changes and aMPV antigen in the nasal turbinate, tracheae, and lung tissues. These results enrich the available information regarding the epidemiology and pathogenicity of aMPV/C in chickens, which may facilitate the development of effective measures against aMPV/C infection in China.http://www.sciencedirect.com/science/article/pii/S0032579122005466avian metapneumovirus subgroup C (aMPV/C)isolation and characterizationrespiratory diseasenucleoprotein gene sequencingpathogenicity |
| spellingShingle | Jing Wang Lei Hou Li Wei Xv Yan Shanshan Zhu Rong Quan Zixuan Li Dan Wang Haijun Jiang Jiangwei Song Yongqiu Cui Jue Liu Characterization of avain metapneumovirus subgroup C isolated from chickens in Beijing, China Poultry Science avian metapneumovirus subgroup C (aMPV/C) isolation and characterization respiratory disease nucleoprotein gene sequencing pathogenicity |
| title | Characterization of avain metapneumovirus subgroup C isolated from chickens in Beijing, China |
| title_full | Characterization of avain metapneumovirus subgroup C isolated from chickens in Beijing, China |
| title_fullStr | Characterization of avain metapneumovirus subgroup C isolated from chickens in Beijing, China |
| title_full_unstemmed | Characterization of avain metapneumovirus subgroup C isolated from chickens in Beijing, China |
| title_short | Characterization of avain metapneumovirus subgroup C isolated from chickens in Beijing, China |
| title_sort | characterization of avain metapneumovirus subgroup c isolated from chickens in beijing china |
| topic | avian metapneumovirus subgroup C (aMPV/C) isolation and characterization respiratory disease nucleoprotein gene sequencing pathogenicity |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122005466 |
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