Oral bacterial composition associated with lung function and lung inflammation in a community-based Norwegian population
Abstract Background The oral cavity is the gateway to the bacteria community in the lung. Disruption of the symbiotic balance of the oral microbiota has been associated with respiratory diseases. However, little is known about the relationship between oral bacteria and respiratory outcomes in the ge...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-07-01
|
| Series: | Respiratory Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12931-023-02491-6 |
| _version_ | 1827899676951576576 |
|---|---|
| author | Rajesh Shigdel Ane Johannessen Huang Lin Shyamal Peddada Francisco Gómez Real Tamar Ringel-Kulka Cecilie Svanes Randi Jacobsen Bertelsen |
| author_facet | Rajesh Shigdel Ane Johannessen Huang Lin Shyamal Peddada Francisco Gómez Real Tamar Ringel-Kulka Cecilie Svanes Randi Jacobsen Bertelsen |
| author_sort | Rajesh Shigdel |
| collection | DOAJ |
| description | Abstract Background The oral cavity is the gateway to the bacteria community in the lung. Disruption of the symbiotic balance of the oral microbiota has been associated with respiratory diseases. However, little is known about the relationship between oral bacteria and respiratory outcomes in the general population. We aimed to describe the associations between oral bacteria, lung function, and lung inflammation in a community-based population. Methods Oral (gingival) samples were collected concurrently with spirometry tests in 477 adults (47% males, median age 28 years) from the RHINESSA study in Bergen, Norway. Bacterial DNA from the 16S rRNA gene from gingival fluid were sequenced by Illumina®MiSeq. Lung function was measured using spirometry and measurement of fractional exhaled nitric oxide (FeNO) were performed to examine airway inflammation. Differential abundance analysis was performed using ANCOM-BC, adjusting for weight, education, and smoking. Results The abundance of the genera Clostridiales , Achromobacter, Moraxella, Flavitalea and Helicobacter were significantly different among those with low FEV1 (< lower limit of normal (LLN)) as compared to normal FEV1 i.e. ≥ LLN. Twenty-three genera differed in abundance between among those with low FVC < LLN as compared to normal FEV1 ≥ LLN. The abundance of 27 genera from phyla Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria and Sacchribacteria differed significantly between elevated FeNO levels (≥ 50 ppb) compared to FeNO ≤ 25 ppb. Conclusion Oral bacterial composition was significantly different for those with low FEV or FVC as compared to those with normal lung function equal to or higher than LLN. Differential bacterial composition was also observed for elevated FeNO levels. |
| first_indexed | 2024-03-12T23:21:05Z |
| format | Article |
| id | doaj.art-3cf2d8db698b456295a1c1c65eaffa61 |
| institution | Directory Open Access Journal |
| issn | 1465-993X |
| language | English |
| last_indexed | 2024-03-12T23:21:05Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-3cf2d8db698b456295a1c1c65eaffa612023-07-16T11:25:30ZengBMCRespiratory Research1465-993X2023-07-0124111210.1186/s12931-023-02491-6Oral bacterial composition associated with lung function and lung inflammation in a community-based Norwegian populationRajesh Shigdel0Ane Johannessen1Huang Lin2Shyamal Peddada3Francisco Gómez Real4Tamar Ringel-Kulka5Cecilie Svanes6Randi Jacobsen Bertelsen7Department of Clinical Science, University of BergenDepartment of Global Public Health and Primary Care, University of BergenEunice Kennedy Shriver National Institute of Child Health and Human DevelopmentEunice Kennedy Shriver National Institute of Child Health and Human DevelopmentDepartment of Clinical Science, University of BergenUNC Gillings School of Global Public Health, Department of Maternal and Child Health, The University of North Carolina at Chapel HillDepartment of Occupational Medicine, Haukeland University HospitalDepartment of Clinical Science, University of BergenAbstract Background The oral cavity is the gateway to the bacteria community in the lung. Disruption of the symbiotic balance of the oral microbiota has been associated with respiratory diseases. However, little is known about the relationship between oral bacteria and respiratory outcomes in the general population. We aimed to describe the associations between oral bacteria, lung function, and lung inflammation in a community-based population. Methods Oral (gingival) samples were collected concurrently with spirometry tests in 477 adults (47% males, median age 28 years) from the RHINESSA study in Bergen, Norway. Bacterial DNA from the 16S rRNA gene from gingival fluid were sequenced by Illumina®MiSeq. Lung function was measured using spirometry and measurement of fractional exhaled nitric oxide (FeNO) were performed to examine airway inflammation. Differential abundance analysis was performed using ANCOM-BC, adjusting for weight, education, and smoking. Results The abundance of the genera Clostridiales , Achromobacter, Moraxella, Flavitalea and Helicobacter were significantly different among those with low FEV1 (< lower limit of normal (LLN)) as compared to normal FEV1 i.e. ≥ LLN. Twenty-three genera differed in abundance between among those with low FVC < LLN as compared to normal FEV1 ≥ LLN. The abundance of 27 genera from phyla Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria and Sacchribacteria differed significantly between elevated FeNO levels (≥ 50 ppb) compared to FeNO ≤ 25 ppb. Conclusion Oral bacterial composition was significantly different for those with low FEV or FVC as compared to those with normal lung function equal to or higher than LLN. Differential bacterial composition was also observed for elevated FeNO levels.https://doi.org/10.1186/s12931-023-02491-6Oral microbiomeLung functionForced expiratory volumeFractional exhaled nitric oxideForced vital capacity |
| spellingShingle | Rajesh Shigdel Ane Johannessen Huang Lin Shyamal Peddada Francisco Gómez Real Tamar Ringel-Kulka Cecilie Svanes Randi Jacobsen Bertelsen Oral bacterial composition associated with lung function and lung inflammation in a community-based Norwegian population Respiratory Research Oral microbiome Lung function Forced expiratory volume Fractional exhaled nitric oxide Forced vital capacity |
| title | Oral bacterial composition associated with lung function and lung inflammation in a community-based Norwegian population |
| title_full | Oral bacterial composition associated with lung function and lung inflammation in a community-based Norwegian population |
| title_fullStr | Oral bacterial composition associated with lung function and lung inflammation in a community-based Norwegian population |
| title_full_unstemmed | Oral bacterial composition associated with lung function and lung inflammation in a community-based Norwegian population |
| title_short | Oral bacterial composition associated with lung function and lung inflammation in a community-based Norwegian population |
| title_sort | oral bacterial composition associated with lung function and lung inflammation in a community based norwegian population |
| topic | Oral microbiome Lung function Forced expiratory volume Fractional exhaled nitric oxide Forced vital capacity |
| url | https://doi.org/10.1186/s12931-023-02491-6 |
| work_keys_str_mv | AT rajeshshigdel oralbacterialcompositionassociatedwithlungfunctionandlunginflammationinacommunitybasednorwegianpopulation AT anejohannessen oralbacterialcompositionassociatedwithlungfunctionandlunginflammationinacommunitybasednorwegianpopulation AT huanglin oralbacterialcompositionassociatedwithlungfunctionandlunginflammationinacommunitybasednorwegianpopulation AT shyamalpeddada oralbacterialcompositionassociatedwithlungfunctionandlunginflammationinacommunitybasednorwegianpopulation AT franciscogomezreal oralbacterialcompositionassociatedwithlungfunctionandlunginflammationinacommunitybasednorwegianpopulation AT tamarringelkulka oralbacterialcompositionassociatedwithlungfunctionandlunginflammationinacommunitybasednorwegianpopulation AT ceciliesvanes oralbacterialcompositionassociatedwithlungfunctionandlunginflammationinacommunitybasednorwegianpopulation AT randijacobsenbertelsen oralbacterialcompositionassociatedwithlungfunctionandlunginflammationinacommunitybasednorwegianpopulation |