PF-4708671 activates AMPK independently of p70S6K1 inhibition.
The P70 ribosomal protein S6 kinase 1 (P70S6K1) is activated by the mammalian target of rapamycin (mTORC1) and regulates proliferation, growth, and metabolism. PF-4708671 is a novel, cell-permeable, has been proposed to be a highly specific inhibitor of p70S6K1. It is used in micromolar concentratio...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4159345?pdf=render |
| _version_ | 1818921674816356352 |
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| author | Gilad W Vainer Ann Saada Juliane Kania-Almog Adir Amartely Jacob Bar-Tana Rachel Hertz |
| author_facet | Gilad W Vainer Ann Saada Juliane Kania-Almog Adir Amartely Jacob Bar-Tana Rachel Hertz |
| author_sort | Gilad W Vainer |
| collection | DOAJ |
| description | The P70 ribosomal protein S6 kinase 1 (P70S6K1) is activated by the mammalian target of rapamycin (mTORC1) and regulates proliferation, growth, and metabolism. PF-4708671 is a novel, cell-permeable, has been proposed to be a highly specific inhibitor of p70S6K1. It is used in micromolar concentration range to dissect signaling pathways downstream of mTORC1 and to study the function of p70S6K1. Here we show that PF-4708671 induces AMP-activated protein kinase (AMPK) phosphorylation and activation in immortalized mouse embryonic fibroblasts (MEF) independently of p70S6K1, due to specific inhibition of mitochondrial respiratory chain Complex I. |
| first_indexed | 2024-12-20T01:41:24Z |
| format | Article |
| id | doaj.art-3cf93284241f408db183ad6b65467b2d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T01:41:24Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3cf93284241f408db183ad6b65467b2d2022-12-21T19:57:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10736410.1371/journal.pone.0107364PF-4708671 activates AMPK independently of p70S6K1 inhibition.Gilad W VainerAnn SaadaJuliane Kania-AlmogAdir AmartelyJacob Bar-TanaRachel HertzThe P70 ribosomal protein S6 kinase 1 (P70S6K1) is activated by the mammalian target of rapamycin (mTORC1) and regulates proliferation, growth, and metabolism. PF-4708671 is a novel, cell-permeable, has been proposed to be a highly specific inhibitor of p70S6K1. It is used in micromolar concentration range to dissect signaling pathways downstream of mTORC1 and to study the function of p70S6K1. Here we show that PF-4708671 induces AMP-activated protein kinase (AMPK) phosphorylation and activation in immortalized mouse embryonic fibroblasts (MEF) independently of p70S6K1, due to specific inhibition of mitochondrial respiratory chain Complex I.http://europepmc.org/articles/PMC4159345?pdf=render |
| spellingShingle | Gilad W Vainer Ann Saada Juliane Kania-Almog Adir Amartely Jacob Bar-Tana Rachel Hertz PF-4708671 activates AMPK independently of p70S6K1 inhibition. PLoS ONE |
| title | PF-4708671 activates AMPK independently of p70S6K1 inhibition. |
| title_full | PF-4708671 activates AMPK independently of p70S6K1 inhibition. |
| title_fullStr | PF-4708671 activates AMPK independently of p70S6K1 inhibition. |
| title_full_unstemmed | PF-4708671 activates AMPK independently of p70S6K1 inhibition. |
| title_short | PF-4708671 activates AMPK independently of p70S6K1 inhibition. |
| title_sort | pf 4708671 activates ampk independently of p70s6k1 inhibition |
| url | http://europepmc.org/articles/PMC4159345?pdf=render |
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